DOI: 10.1002/pro.5560011212. Functional mapping of the surface of escherichia coli ribose‐binding protein: Mutations that affect chemotaxis and transport

Functional mapping of the surface of escherichia coli ribose‐binding protein: Mutations that affect chemotaxis and transport

10.1002/pro.5560011212

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractRibose‐binding protein is a bifunctional soluble receptor found in the periplasm of Escherichia coli. Interaction of liganded binding protein with the ribose high affinity transport complex results in the transfer of ribose across the cytoplasmic membrane. Alternatively, interaction of liganded binding protein with a chemotactic signal transducer, Trg, initiates taxis toward ribose. We have generated a functional map of the surface of ribose‐binding protein by creating and analyzing directed mutations of exposed residues. Residues in an area on the cleft side of the molecule including both domains have effects on transport. A portion of the area involved in transport is also essential to chemotactic function. On the opposite face of the protein, mutations in residues near the hinge are shown to affect chemotaxis specifically.

Bibliography

Binnie1, R. A., Zhang, H., Mowbray, S., & Hermodson, M. A. (1992). Functional mapping of the surface of escherichia coli riboseâbinding protein: Mutations that affect chemotaxis and transport. Protein Science, 1(12), 1642â1651. Portico.

Authors 4

R. Alan Binnie1 (first)
Huide Zhang (additional)
Sherry Mowbray (additional)
Mark A. Hermodson (additional)

References 38 Referenced 42

10.1126/science.153.3737.708
10.1146/annurev.bi.55.070186.002145
10.1128/jb.160.3.1181-1183.1984 / J. Bacteriol. / Simple, rapid and quantitative release of periplasmic proteins by chloroform by Ames G.F. (1984)
10.1016/S0021-9258(19)69442-1 / J. Biol. Chem. / Structural prediction of sugar binding proteins functional in chemotaxis and transport by Argos P. (1981)
10.1073/pnas.81.11.3287
10.1042/bj1390715
10.1128/jb.174.5.1528-1536.1992
10.1016/S0021-9258(17)44063-4 / J. Biol. Chem. / The amino acid sequence of the d‐ribose‐binding protein from E. coli K 12 by Groarke J.M. (1983)
10.1073/pnas.64.4.1300
10.1098/rstb.1990.0017
10.1016/S0021-9258(17)40167-0 / J. Biol. Chem. / Amino acid sequence of the l‐arabinose‐binding protein from E. coli B/r by Hogg R.W. (1977)
10.1038/346362a0
10.1128/jb.158.2.674-682.1984 / J. Bacteriol. / Molecular cloning and characterization of genes required for ribose transport and utilization in E. coli K‐12 by Iida A. (1984)
10.1007/978-1-4612-3374-9_13
10.1002/pro.5560011213
10.1073/pnas.76.1.260
10.1128/jb.170.10.4516-4521.1988 / J. Bacteriol. / Maltose chemoreceptor of E. coli: Interaction of maltose‐binding protein and the Tar signal transducer by Kossman M. (1988)
10.1107/S0021889891004399
10.1016/S0021-9258(19)69441-X / J. Biol. Chem. / Amino acid sequence of the d‐galactose‐binding protein from E. coli B/r by Mahoney W.C. (1981)
10.1126/science.1660187
{'key': 'e_1_2_1_22_1', 'volume-title': 'Experiments in Molecular Genetics', 'author': 'Miller J.H.', 'year': '1972'} / Experiments in Molecular Genetics by Miller J.H. (1972)
{'key': 'e_1_2_1_23_1', 'first-page': '163', 'volume-title': 'Microbial Energy Transduction: Genetics, Structure and Function of Membrane Proteins', 'author': 'Moe G.R.', 'year': '1986'} / Microbial Energy Transduction: Genetics, Structure and Function of Membrane Proteins by Moe G.R. (1986)
10.1016/0022-2836(92)90898-T
10.1016/0022-2836(92)91033-L
{'key': 'e_1_2_1_26_1', 'first-page': '41', 'article-title': 'Structure of the periplasmic glucose/galactose receptor of Salmonella typhimurium', 'volume': '1', 'author': 'Mowbray S.L.', 'year': '1990', 'journal-title': 'Receptors'} / Receptors / Structure of the periplasmic glucose/galactose receptor of Salmonella typhimurium by Mowbray S.L. (1990)
10.1128/jb.117.2.509-516.1974 / J. Bacteriol. / Isolation and complementation of mutants in galactose taxis and transport by Ordal G.W. (1974)
10.1128/jb.167.1.101-109.1986
10.1038/310381a0
10.1016/0378-1119(87)90127-2
10.1111/j.1749-6632.1949.tb27297.x
10.1007/BF00330450
10.1016/S0021-9258(19)67774-4 / J. Biol. Chem. / The 2.3 Ångstrom resolution structure of the maltose‐ or maltodextrin‐binding protein, a primary receptor of bacterial active transport and chemotaxis by Spurlino J.C. (1991)
10.1073/pnas.73.3.762
10.1016/0022-2836(86)90385-2
10.1016/0076-6879(87)53044-0
10.1126/science.3057628
10.1016/S0021-9258(19)67776-8 / J. Biol. Chem. / Comparison of the periplasmic receptors for l‐arabinose, d‐glucose/d‐galactose and d‐ribose by Vyas N.K. (1991)
10.1016/S0021-9258(18)50020-X

Dates

Type	When
Created	16 years, 6 months ago (Feb. 9, 2009, 6:23 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 2:49 p.m.)
Indexed	1 year ago (Aug. 21, 2024, 8:12 p.m.)
Issued	32 years, 8 months ago (Dec. 1, 1992)
Published	32 years, 8 months ago (Dec. 1, 1992)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	32 years, 8 months ago (Dec. 1, 1992)

Funders 0

None

BibTeX

@article{Binnie1_1992, title={Functional mapping of the surface of escherichia coli ribose‐binding protein: Mutations that affect chemotaxis and transport}, volume={1}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560011212}, DOI={10.1002/pro.5560011212}, number={12}, journal={Protein Science}, publisher={Wiley}, author={Binnie1, R. Alan and Zhang, Huide and Mowbray, Sherry and Hermodson, Mark A.}, year={1992}, month=dec, pages={1642–1651} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        22
      ]
    ],
    "date-time": "2024-08-22T00:12:08Z",
    "timestamp": 1724285528980
  },
  "reference-count": 38,
  "publisher": "Wiley",
  "issue": "12",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            12,
            31
          ]
        ],
        "date-time": "2008-12-31T00:00:00Z",
        "timestamp": 1230681600000
      },
      "content-version": "vor",
      "delay-in-days": 5874,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1992,
        12
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Ribose\u2010binding protein is a bifunctional soluble receptor found in the periplasm of <jats:italic>Escherichia coli.</jats:italic> Interaction of liganded binding protein with the ribose high affinity transport complex results in the transfer of ribose across the cytoplasmic membrane. Alternatively, interaction of liganded binding protein with a chemotactic signal transducer, Trg, initiates taxis toward ribose. We have generated a functional map of the surface of ribose\u2010binding protein by creating and analyzing directed mutations of exposed residues. Residues in an area on the cleft side of the molecule including both domains have effects on transport. A portion of the area involved in transport is also essential to chemotactic function. On the opposite face of the protein, mutations in residues near the hinge are shown to affect chemotaxis specifically.</jats:p>",
  "DOI": "10.1002/pro.5560011212",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        2,
        9
      ]
    ],
    "date-time": "2009-02-09T23:23:15Z",
    "timestamp": 1234221795000
  },
  "page": "1642-1651",
  "source": "Crossref",
  "is-referenced-by-count": 42,
  "title": "Functional mapping of the surface of escherichia coli ribose\u2010binding protein: Mutations that affect chemotaxis and transport",
  "prefix": "10.1002",
  "volume": "1",
  "author": [
    {
      "given": "R. Alan",
      "family": "Binnie1",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Huide",
      "family": "Zhang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sherry",
      "family": "Mowbray",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Mark A.",
      "family": "Hermodson",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        12,
        31
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.153.3737.708"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.55.070186.002145"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "1181",
      "DOI": "10.1128/jb.160.3.1181-1183.1984",
      "article-title": "Simple, rapid and quantitative release of periplasmic proteins by chloroform",
      "volume": "160",
      "author": "Ames G.F.",
      "year": "1984",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "4357",
      "DOI": "10.1016/S0021-9258(19)69442-1",
      "article-title": "Structural prediction of sugar binding proteins functional in chemotaxis and transport",
      "volume": "256",
      "author": "Argos P.",
      "year": "1981",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.81.11.3287"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1390715"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.174.5.1528-1536.1992"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "12952",
      "DOI": "10.1016/S0021-9258(17)44063-4",
      "article-title": "The amino acid sequence of the d\u2010ribose\u2010binding protein from E. coli K 12",
      "volume": "258",
      "author": "Groarke J.M.",
      "year": "1983",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.64.4.1300"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rstb.1990.0017"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "5135",
      "DOI": "10.1016/S0021-9258(17)40167-0",
      "article-title": "Amino acid sequence of the l\u2010arabinose\u2010binding protein from E. coli B/r",
      "volume": "252",
      "author": "Hogg R.W.",
      "year": "1977",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/346362a0"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "674",
      "DOI": "10.1128/jb.158.2.674-682.1984",
      "article-title": "Molecular cloning and characterization of genes required for ribose transport and utilization in E. coli K\u201012",
      "volume": "158",
      "author": "Iida A.",
      "year": "1984",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-1-4612-3374-9_13"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pro.5560011213"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.1.260"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "4516",
      "DOI": "10.1128/jb.170.10.4516-4521.1988",
      "article-title": "Maltose chemoreceptor of E. coli: Interaction of maltose\u2010binding protein and the Tar signal transducer",
      "volume": "170",
      "author": "Kossman M.",
      "year": "1988",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0021889891004399"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "4350",
      "DOI": "10.1016/S0021-9258(19)69441-X",
      "article-title": "Amino acid sequence of the d\u2010galactose\u2010binding protein from E. coli B/r",
      "volume": "256",
      "author": "Mahoney W.C.",
      "year": "1981",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1660187"
    },
    {
      "key": "e_1_2_1_22_1",
      "volume-title": "Experiments in Molecular Genetics",
      "author": "Miller J.H.",
      "year": "1972"
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "163",
      "volume-title": "Microbial Energy Transduction: Genetics, Structure and Function of Membrane Proteins",
      "author": "Moe G.R.",
      "year": "1986"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(92)90898-T"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(92)91033-L"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "41",
      "article-title": "Structure of the periplasmic glucose/galactose receptor of Salmonella typhimurium",
      "volume": "1",
      "author": "Mowbray S.L.",
      "year": "1990",
      "journal-title": "Receptors"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "crossref",
      "first-page": "509",
      "DOI": "10.1128/jb.117.2.509-516.1974",
      "article-title": "Isolation and complementation of mutants in galactose taxis and transport",
      "volume": "117",
      "author": "Ordal G.W.",
      "year": "1974",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.167.1.101-109.1986"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/310381a0"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(87)90127-2"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1749-6632.1949.tb27297.x"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00330450"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "crossref",
      "first-page": "5202",
      "DOI": "10.1016/S0021-9258(19)67774-4",
      "article-title": "The 2.3 \u00c5ngstrom resolution structure of the maltose\u2010 or maltodextrin\u2010binding protein, a primary receptor of bacterial active transport and chemotaxis",
      "volume": "266",
      "author": "Spurlino J.C.",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.73.3.762"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(86)90385-2"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(87)53044-0"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3057628"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "crossref",
      "first-page": "5226",
      "DOI": "10.1016/S0021-9258(19)67776-8",
      "article-title": "Comparison of the periplasmic receptors for l\u2010arabinose, d\u2010glucose/d\u2010galactose and d\u2010ribose",
      "volume": "266",
      "author": "Vyas N.K.",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)50020-X"
    }
  ],
  "container-title": "Protein Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpro.5560011212",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/pro.5560011212",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        23
      ]
    ],
    "date-time": "2023-10-23T18:49:16Z",
    "timestamp": 1698086956000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/pro.5560011212"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1992,
        12
      ]
    ]
  },
  "references-count": 38,
  "journal-issue": {
    "issue": "12",
    "published-print": {
      "date-parts": [
        [
          1992,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/pro.5560011212"
  ],
  "URL": "http://dx.doi.org/10.1002/pro.5560011212",
  "relation": {},
  "ISSN": [
    "0961-8368",
    "1469-896X"
  ],
  "subject": [],
  "container-title-short": "Protein Science",
  "published": {
    "date-parts": [
      [
        1992,
        12
      ]
    ]
  }
}