Differentiation between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins
10.1002/pro.5560010809
Crossref journal-article
Wiley
Protein Science (311)
Abstract

AbstractThe interpretation of the circular dichroism (CD) spectra of proteins to date requires additional secondary structural information of the proteins to be analyzed, such as X‐ray or NMR data. Therefore, these methods are inappropriate for a CD database whose secondary structures are unknown, as in the case of the membrane proteins. The convex constraint analysis algorithm (Perczel, A., Hollósi, M., Tusnády, G., & Fasman, G.D., 1991, Protein Eng. 4, 669–679), on the other hand, operates only on a collection of spectral data to extract the common spectral components with their spectral weights. The linear combinations of these derived “pure” CD curves can reconstruct the original data set with great accuracy. For a membrane protein data set, the five‐component spectra so obtained from the deconvolution consisted of two different types of α helices (the α helix in the soluble domain and the αT helix, for the transmembrane α helix), a β‐pleated sheet, a class C‐like spectrum related to β turns, and a spectrum correlated with the unordered conformation. The deconvoluted CD spectrum for the αT helix was characterized by a positive red‐shifted band in the range 195–200 nm (+95,000 deg cm2 dmol−1), with the intensity of the negative band at 208 nm being slightly less negative than that of the 222‐nm band (–50,000 and −60,000 deg cm2 dmol−1, respectively) in comparison with the regular α helix, with a positive band at 190 nm and two negative bands at 208 and 222 nm with magnitudes of +70,000, ‐30,000, and ‐30,000 deg cm2 dmol−1, respectively.

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Park, K., Perczel, A., & Fasman, G. D. (1992). Differentiation between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins. Protein Science, 1(8), 1032–1049. Portico.

Authors 3
  1. Kyusung Park (first)
  2. András Perczel (additional)
  3. Gerald D. Fasman (additional)
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Dates
Type When
Created 16 years, 6 months ago (Feb. 9, 2009, 6:40 p.m.)
Deposited 1 year, 10 months ago (Oct. 23, 2023, 7:48 a.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued 33 years ago (Aug. 1, 1992)
Published 33 years ago (Aug. 1, 1992)
Published Online 16 years, 7 months ago (Dec. 31, 2008)
Published Print 33 years ago (Aug. 1, 1992)
Funders 0

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@article{Park_1992, title={Differentiation between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins}, volume={1}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560010809}, DOI={10.1002/pro.5560010809}, number={8}, journal={Protein Science}, publisher={Wiley}, author={Park, Kyusung and Perczel, András and Fasman, Gerald D.}, year={1992}, month=aug, pages={1032–1049} }