DOI: 10.1002/pro.5560010310. Quantitative analysis of cyclic β‐turn models

Quantitative analysis of cyclic β‐turn models

10.1002/pro.5560010310

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe β‐turn is a frequently found structural unit in the conformation of globular proteins. Although the circular dichroism (CD) spectra of the α‐helix and β‐pleated sheet are well defined, there remains some ambiguity concerning the pure component CD spectra of the different types of β‐turns. Recently, it has been reported (Hollósi, M., Koövér, K.E., Holly, S., Radics, L., & Fasman, G.D., 1987, Biopolymers 26, 1527–1572; Perczel, A., Hollósi, M., Foxman, B.M., & Fasman, G.D., 1991a, J. Am. Chem. Soc. 113, 9772–9784) that some pseudohexapeptides (e.g., the cyclo[(δ)Ava–Gly–Pro–Aaa–Gly] where Aaa = Ser, Ser(OtBu), or Gly) in many solvents adopt a conformational mixture of type I and the type II β‐turns, although the X‐ray‐determined conformation was an ideal type I β‐turn. In addition to these pseudohexapeptides, conformational analysis was also carried out on three pseudotetrapeptides and three pseudooctapeptides. The target of the conformation analysis reported herein was to determine whether the ring stress of the above β‐turn models has an influence on their conformational properties.Quantitative nuclear Overhauser effect (NOE) measurements yielded interproton distances. The conformational average distances so obtained were interpreted utilizing molecular dynamics (MD) simulations to yield the conformational percentages. These conformational ratios were correlated with the conformational weights obtained by quantitative CD analysis of the same compounds. The pure component CD curves of type I and type II β‐turns were also obtained, using a recently developed algorithm (Perczel, A., Tusnády, G., Hollósi, M., & Fasman, G.D., 1991b, Protein Eng. 4(6), 669–679). For the first time the results of a CD deconvolution, based on the CD spectra of 14 β‐turn models, were assigned by quantitative NOE results. The NOE experiments confirmed the ratios of the component curves found for the two major β‐turns by CD analysis. These results can now be used to enhance the conformational determination of globular proteins on the basis of their CD spectra.

Bibliography

Perczel, A., & Fasman, G. D. (1992). Quantitative analysis of cyclic Î²âturn models. Protein Science, 1(3), 378â395. Portico.

Authors 2

Andraás Perczel (first)
Gerald D. Fasman (additional)

References 46 Referenced 110

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Dates

Type	When
Created	15 years, 1 month ago (July 12, 2010, 1:11 a.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 7:17 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 2, 2025, 12:29 a.m.)
Issued	33 years, 5 months ago (March 1, 1992)
Published	33 years, 5 months ago (March 1, 1992)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	33 years, 5 months ago (March 1, 1992)

Funders 1

NSF
Awards1
1. DMB-9007055)

BibTeX

@article{Perczel_1992, title={Quantitative analysis of cyclic β‐turn models}, volume={1}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560010310}, DOI={10.1002/pro.5560010310}, number={3}, journal={Protein Science}, publisher={Wiley}, author={Perczel, Andraás and Fasman, Gerald D.}, year={1992}, month=mar, pages={378–395} }

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