DOI: 10.1002/pmic.200800722. Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7

Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7

10.1002/pmic.200800722

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractThe rough ER (rER) plays a central role in the biogenesis of most extracellular and many organellar proteins in eukaryotic cells. Cells that are specialized in protein secretion, such as pancreatic cells, are particularly rich in rER. In the process of cell homogenization, the rER is converted into ribosome‐studded vesicles, the so‐called rough microsomes. Here we report on a membrane proteomic analysis of canine pancreatic rough microsomes. Special emphasis was placed on components involved in the various aspects of protein biogenesis, such as protein transport, protein folding, protein modification, and protein degradation. Our results indicate that the Hsp70‐chaperone network that is present in the pancreatic ER is even more complex than previously thought, and suggest that the pancreatic rER has a significant capacity for protein degradation.

Bibliography

Zahedi, R. P., VÃ¶lzing, C., Schmitt, A., Frien, M., Jung, M., Dudek, J., Wortelkamp, S., Sickmann, A., & Zimmermann, R. (2009). Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7. PROTEOMICS, 9(13), 3463â3473. Portico.

Authors 9

René P. Zahedi (first)
Christian Völzing (additional)
Andreas Schmitt (additional)
Michael Frien (additional)
Martin Jung (additional)
Johanna Dudek (additional)
Stefanie Wortelkamp (additional)
Albert Sickmann (additional)
Richard Zimmermann (additional)

References 37 Referenced 29

10.1126/science.1096303
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{'key': 'e_1_2_6_4_2', 'first-page': '205', 'volume-title': 'Molecular Biology of Chaperones and Folding Catalysts', 'author': 'Haas I.', 'year': '1999'} / Molecular Biology of Chaperones and Folding Catalysts by Haas I. (1999)
10.1016/j.cell.2007.06.005
10.1016/S0076-6879(83)96010-X
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Dates

Type	When
Created	16 years, 2 months ago (July 3, 2009, 9:39 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 7:31 p.m.)
Indexed	2 months ago (July 2, 2025, 2:33 p.m.)
Issued	16 years, 2 months ago (July 1, 2009)
Published	16 years, 2 months ago (July 1, 2009)
Published Online	16 years, 1 month ago (July 16, 2009)
Published Print	16 years, 2 months ago (July 1, 2009)

Funders 2

Deutsche Forschungsgemeinschaft 10.13039/501100001659
Region: Europe
gov (National government)
Labels3
1. German Research Association
2. German Research Foundation
3. DFG
Awards2
1. SFB 530
2. FZT-82
Saarland Ministry for Economic Affairs and Science

BibTeX

@article{Zahedi_2009, title={Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7}, volume={9}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200800722}, DOI={10.1002/pmic.200800722}, number={13}, journal={PROTEOMICS}, publisher={Wiley}, author={Zahedi, René P. and Völzing, Christian and Schmitt, Andreas and Frien, Michael and Jung, Martin and Dudek, Johanna and Wortelkamp, Stefanie and Sickmann, Albert and Zimmermann, Richard}, year={2009}, month=jul, pages={3463–3473} }

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