DOI: 10.1002/pmic.200700620. Proteotypic profiling of LHCI from <b><i>Chlamydomonas reinhardtii</i></b> provides new insights into structure and function of the complex

Proteotypic profiling of LHCI from Chlamydomonas reinhardtii provides new insights into structure and function of the complex

10.1002/pmic.200700620

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractWe used isotope dilution MS to measure the stoichiometry of light‐harvesting complex I (LHCI) proteins with the photosystem I (PSI) core complex in the green alga Chlamydomonas reinhardtii. Proteotypic peptides served as quantitative markers for each of the nine gene products (Lhca1–9) and for PSI subunits. The quantitative data revealed that the LHCI antenna of C. reinhardtii contains about 7.5 ± 1.4 subunits. It further demonstrated that the thylakoid LHCI population is heterogeneously composed and that several lhca gene products are not present in 1:1 stoichiometries with PSI. When compared with vascular plants, LHCI of C. reinhardtii possesses a lower proportion of proteins potentially contributing to far‐red fluorescence emission. In general, the strategy presented is universally applicable for exploring subunit stoichiometries within the C. reinhardtii proteome.

Bibliography

Stauber, E. J., Busch, A., Naumann, B., SvatoÅ¡, A., & Hippler, M. (2009). Proteotypic profiling of LHCI from Chlamydomonas reinhardtii provides new insights into structure and function of the complex. PROTEOMICS, 9(2), 398â408. Portico.

Authors 5

Einar J. Stauber (first)
Andreas Busch (additional)
Bianca Naumann (additional)
Aleš Svatoš (additional)
Michael Hippler (additional)

References 65 Referenced 34

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Dates

Type	When
Created	16 years, 7 months ago (Jan. 13, 2009, 8:53 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 11:40 a.m.)
Indexed	6 days, 18 hours ago (Aug. 29, 2025, 6:23 a.m.)
Issued	16 years, 8 months ago (Jan. 1, 2009)
Published	16 years, 8 months ago (Jan. 1, 2009)
Published Online	16 years, 7 months ago (Jan. 13, 2009)
Published Print	16 years, 8 months ago (Jan. 1, 2009)

Funders 0

None

BibTeX

@article{Stauber_2009, title={Proteotypic profiling of LHCI from Chlamydomonas reinhardtii provides new insights into structure and function of the complex}, volume={9}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200700620}, DOI={10.1002/pmic.200700620}, number={2}, journal={PROTEOMICS}, publisher={Wiley}, author={Stauber, Einar J. and Busch, Andreas and Naumann, Bianca and Svatoš, Aleš and Hippler, Michael}, year={2009}, month=jan, pages={398–408} }

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