DOI: 10.1002/pmic.200700588. Subunit–subunit interactions in the human 26S proteasome

Subunit–subunit interactions in the human 26S proteasome

10.1002/pmic.200700588

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractUbiquitin‐dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two‐hybrid analysis of their interactions with each other. We observed that there are 114 interacting‐pairs in the human 26S proteasome. About 10% (11/114) of these interacting‐pairs was confirmed by the GST‐pull down analysis. Among these observed interacting subunits, 58% (66/114) are novel and the rest 42% (48/114) has been reported previously in human or in other species. We observed new interactions between the 19S regulatory particle and the β‐rings of the 20S catalytic particle and therefore proposed a modified model of the 26S proteasome.

Bibliography

Chen, C., Huang, C., Chen, S., Liang, J., Lin, W., Ke, G., Zhang, H., Wang, B., Huang, J., Han, Z., Ma, L., Huo, K., Yang, X., Yang, P., He, F., & Tao, T. (2008). Subunitâsubunit interactions in the human 26S proteasome. PROTEOMICS, 8(3), 508â520. Portico.

Authors 16

Chuan Chen (first)
Caoxin Huang (additional)
Shouhui Chen (additional)
Jie Liang (additional)
Wenbo Lin (additional)
Guifen Ke (additional)
Hongxin Zhang (additional)
Bing Wang (additional)
Jian Huang (additional)
Zeguang Han (additional)
Lixin Ma (additional)
Keke Huo (additional)
Xiaoming Yang (additional)
Pengyuan Yang (additional)
Fuchu He (additional)
Tao Tao (additional)

References 70 Referenced 24

Dates

Type	When
Created	17 years, 7 months ago (Jan. 9, 2008, 8:50 a.m.)
Deposited	1 year, 9 months ago (Nov. 14, 2023, 6:40 p.m.)
Indexed	1 year, 9 months ago (Nov. 14, 2023, 7:18 p.m.)
Issued	17 years, 6 months ago (Jan. 29, 2008)
Published	17 years, 6 months ago (Jan. 29, 2008)
Published Online	17 years, 6 months ago (Jan. 29, 2008)
Published Print	17 years, 6 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Chen_2008, title={Subunit–subunit interactions in the human 26S proteasome}, volume={8}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200700588}, DOI={10.1002/pmic.200700588}, number={3}, journal={PROTEOMICS}, publisher={Wiley}, author={Chen, Chuan and Huang, Caoxin and Chen, Shouhui and Liang, Jie and Lin, Wenbo and Ke, Guifen and Zhang, Hongxin and Wang, Bing and Huang, Jian and Han, Zeguang and Ma, Lixin and Huo, Keke and Yang, Xiaoming and Yang, Pengyuan and He, Fuchu and Tao, Tao}, year={2008}, month=jan, pages={508–520} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        15
      ]
    ],
    "date-time": "2023-11-15T00:18:43Z",
    "timestamp": 1700007523082
  },
  "reference-count": 70,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            1,
            9
          ]
        ],
        "date-time": "2008-01-09T00:00:00Z",
        "timestamp": 1199836800000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "analyticalsciencejournals.onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2008,
        2
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Ubiquitin\u2010dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two\u2010hybrid analysis of their interactions with each other. We observed that there are 114 interacting\u2010pairs in the human 26S proteasome. About 10% (11/114) of these interacting\u2010pairs was confirmed by the GST\u2010pull down analysis. Among these observed interacting subunits, 58% (66/114) are novel and the rest 42% (48/114) has been reported previously in human or in other species. We observed new interactions between the 19S regulatory particle and the \u03b2\u2010rings of the 20S catalytic particle and therefore proposed a modified model of the 26S proteasome.</jats:p>",
  "DOI": "10.1002/pmic.200700588",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        1,
        9
      ]
    ],
    "date-time": "2008-01-09T13:50:45Z",
    "timestamp": 1199886645000
  },
  "page": "508-520",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 24,
  "title": "Subunit\u2013subunit interactions in the human 26S proteasome",
  "prefix": "10.1002",
  "volume": "8",
  "author": [
    {
      "given": "Chuan",
      "family": "Chen",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Caoxin",
      "family": "Huang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Shouhui",
      "family": "Chen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jie",
      "family": "Liang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Wenbo",
      "family": "Lin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Guifen",
      "family": "Ke",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hongxin",
      "family": "Zhang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Bing",
      "family": "Wang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jian",
      "family": "Huang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Zeguang",
      "family": "Han",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lixin",
      "family": "Ma",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Keke",
      "family": "Huo",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Xiaoming",
      "family": "Yang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Pengyuan",
      "family": "Yang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Fuchu",
      "family": "He",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tao",
      "family": "Tao",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        1,
        29
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.00027.2001"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.biochem.68.1.1015"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1069490"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm1336"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2006.10.045"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.24.7096"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pbio.0040267"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1006991419464"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0305411101"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00250-7"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7725097"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386463a0"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(02)00748-7"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature05512"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pmic.200600574"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.e06-07-0635"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/embo-reports/kve184"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35001009"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physiolgenomics.2001.7.1.27"
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.061034498"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/415180a"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M209420200"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M409364200"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.2.875"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(99)01529-7"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/abbi.2000.2178"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi001815n"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M204982200"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.febslet.2004.05.067"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbrc.2003.12.119"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature04209"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2005.08.029"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1456306"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1998.3958"
    },
    {
      "key": "e_1_2_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0958-1669(96)80111-X"
    },
    {
      "key": "e_1_2_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.7.2939"
    },
    {
      "key": "e_1_2_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature04106"
    },
    {
      "key": "e_1_2_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00018-004-4130-z"
    },
    {
      "key": "e_1_2_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/mcp.M500303-MCP200"
    },
    {
      "key": "e_1_2_1_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi061994u"
    },
    {
      "key": "e_1_2_1_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.febslet.2005.04.048"
    },
    {
      "key": "e_1_2_1_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/BJ20011520"
    },
    {
      "key": "e_1_2_1_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.e06-07-0635"
    },
    {
      "key": "e_1_2_1_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.0000237389.40000.02"
    },
    {
      "key": "e_1_2_1_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj20031122"
    },
    {
      "key": "e_1_2_1_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tibs.2005.02.004"
    },
    {
      "key": "e_1_2_1_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.1.171"
    },
    {
      "key": "e_1_2_1_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng1747"
    },
    {
      "key": "e_1_2_1_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.187.1.97"
    },
    {
      "key": "e_1_2_1_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.17.5363"
    },
    {
      "key": "e_1_2_1_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M308757200"
    },
    {
      "key": "e_1_2_1_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.12.2487"
    },
    {
      "key": "e_1_2_1_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M910378199"
    },
    {
      "key": "e_1_2_1_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.20.13403"
    },
    {
      "key": "e_1_2_1_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.febslet.2004.03.073"
    },
    {
      "key": "e_1_2_1_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81603-7"
    },
    {
      "key": "e_1_2_1_58_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.19.10.6575"
    },
    {
      "key": "e_1_2_1_59_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004380051069"
    },
    {
      "key": "e_1_2_1_60_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.6.3112"
    },
    {
      "key": "e_1_2_1_61_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.1334"
    },
    {
      "key": "e_1_2_1_62_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/71600"
    },
    {
      "key": "e_1_2_1_63_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M310265200"
    },
    {
      "key": "e_1_2_1_64_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.ccr.2005.06.006"
    },
    {
      "key": "e_1_2_1_65_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tcb.2006.03.001"
    },
    {
      "key": "e_1_2_1_66_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M107313200"
    },
    {
      "key": "e_1_2_1_67_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.str.2006.11.015"
    },
    {
      "key": "e_1_2_1_68_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.0947"
    },
    {
      "key": "e_1_2_1_69_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.1429"
    },
    {
      "key": "e_1_2_1_70_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M314231200"
    },
    {
      "key": "e_1_2_1_71_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb07313.x"
    }
  ],
  "container-title": "PROTEOMICS",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpmic.200700588",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/full/10.1002/pmic.200700588",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        14
      ]
    ],
    "date-time": "2023-11-14T23:40:24Z",
    "timestamp": 1700005224000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200700588"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2008,
        1,
        29
      ]
    ]
  },
  "references-count": 70,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2008,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/pmic.200700588"
  ],
  "URL": "http://dx.doi.org/10.1002/pmic.200700588",
  "relation": {},
  "ISSN": [
    "1615-9853",
    "1615-9861"
  ],
  "subject": [],
  "container-title-short": "Proteomics",
  "published": {
    "date-parts": [
      [
        2008,
        1,
        29
      ]
    ]
  },
  "assertion": [
    {
      "value": "2007-06-22",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2008-01-29",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}