DOI: 10.1002/pmic.200700244. Proteomic analysis of the proteins regulated by HrpB from the plant pathogenic bacterium <b><i>Burkholderia glumae</i></b>

Proteomic analysis of the proteins regulated by HrpB from the plant pathogenic bacterium Burkholderia glumae

10.1002/pmic.200700244

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractPlant pathogenic bacteria transfer effector proteins into plant cells via the hypersensitive response and pathogenicity (Hrp) type III protein secretion system (T3SS) during infection. The genes encoding the Hrp T3SS are expressed only under plant apoplast‐mimicking conditions in an AraC‐type transcriptional activator HrpB‐dependent manner. To identify the proteins controlled by HrpB in Burkholderia glumae in vitro, we constitutively expressed hrpB and analyzed the proteins showing altered expression using 2‐DE and ESI‐MS/MS. Among 46 proteins exhibiting consistently altered expression, which were encoded by 34 different genes, 34 were secretory proteins and 12 were cytoplasmic. Twenty‐eight of the secreted proteins showed increased accumulation, whereas the other six showed decreased accumulation. None of the HrpB‐dependent proteins had significant homology to known T3SS‐dependent proteins, except for HrpK from Pseudomonas syringae pv. syringae and two T3SS‐associated cytoplasmic proteins from Ralstonia solanacearum. Twenty‐one of the 34 genes had putative HrpB‐binding sequences in their upstream regulatory regions. Secretion of all 34 extracellular proteins was independent of the Hrp T3SS, and 16 were secreted via a type II protein secretion system (T2SS). Mutants lacking the T2SS or Hrp T3SS produced toxoflavin but were less virulent to rice panicles, indicating the importance of these proteins in pathogenicity.

Bibliography

Kang, Y., Kim, J., Kim, S., Kim, H., Lim, J. Y., Kim, M., Kwak, J., Moon, J. S., & Hwang, I. (2007). Proteomic analysis of the proteins regulated by HrpB from the plant pathogenic bacterium Burkholderia glumae. PROTEOMICS, 8(1), 106â121. Portico.

Authors 9

Yongsung Kang (first)
Jinwoo Kim (additional)
Suhyun Kim (additional)
Hongsup Kim (additional)
Jae Yun Lim (additional)
Minkyun Kim (additional)
Jangyul Kwak (additional)
Jae Sun Moon (additional)
Ingyu Hwang (additional)

References 64 Referenced 41

10.1094/PD-80-0429
10.1094/PDIS.2003.87.8.890
10.1099/00207713-40-3-309
10.3186/jjphytopath.62.108
10.4241/kyubyochu.33.9
10.1111/j.1365-2958.2004.04338.x
10.1146/annurev.micro.54.1.735
10.1128/JB.179.18.5655-5662.1997
10.1046/j.1365-2958.1996.5731077.x
10.1126/science.284.5418.1322
10.1073/pnas.92.26.11998
10.1016/S0092-8674(03)00036-9
10.1146/annurev.arplant.56.032604.144218
10.1073/pnas.032514099
10.1046/j.1365-2958.2002.02964.x
10.1073/pnas.112183899
10.1002/1615-9861(200209)2:9<1325::AID-PROT1325>3.0.CO;2-4
10.1002/pmic.200300814
10.1094/MPMI.2004.17.6.593
10.1128/JB.186.5.1374-1380.2004
10.1094/MPMI-18-0891
10.1016/j.pbi.2005.05.004
10.1046/j.1365-2958.2003.03425.x
10.1105/tpc.104.026765
10.1128/JB.174.11.3499-3507.1992
10.1128/JB.174.6.1875-1882.1992
10.1128/jb.178.4.1061-1069.1996
10.1146/annurev.phyto.38.1.263
10.1128/JB.178.12.3462-3469.1996
10.1094/MPMI.2003.16.3.238
10.1128/JB.176.10.3089-3091.1994
10.1128/JB.186.8.2309-2318.2004
10.1046/j.1365-2958.2001.02567.x
10.1128/JB.00795-06
10.1128/AEM.39.1.25-29.1980
{'key': 'e_1_2_1_37_2', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual by Sambrook J. (1989)
10.1073/pnas.76.4.1648
10.1038/nbt1183-784 / Bio/Technol. / A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram‐negative bacteria by Simon R. (1983)
10.1002/j.1460-2075.1985.tb03715.x
10.1128/jb.169.12.5789-5794.1987
10.1126/science.2781284
10.1007/BF00330575
10.1016/0378-1119(84)90059-3
10.1046/j.1365-2958.1998.00671.x
10.1016/0378-1119(89)90162-5
10.1002/j.1460-2075.1987.tb02730.x
10.3186/jjphytopath.61.470
10.1016/0003-2697(76)90527-3
10.1002/elps.11501601183
10.1016/S0022-2836(05)80360-2
10.1016/0092-8674(93)90354-S
10.1128/jb.178.21.6399-6402.1996
10.1073/pnas.97.13.7539
10.1046/j.1365-2958.2000.01902.x
10.1093/emboj/cdf536
10.1111/j.1365-2958.1995.mmi_17050889.x
10.1073/pnas.92.14.6625
10.1128/JB.187.2.649-663.2005
10.1006/pmpp.1999.0225
10.1093/emboj/20.23.6735 / EMBO J. / Involvement of the twin‐arginine translocation system in protein secretion via the type II pathway by Voulhoux R. (2001)
10.1126/science.1128393
10.1128/JB.182.1.248-251.2000
10.1074/jbc.273.41.26670
10.1128/IAI.69.6.3523-3535.2001

Dates

Type	When
Created	17 years, 9 months ago (Nov. 29, 2007, 1:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 14, 2023, 8:07 p.m.)
Indexed	2 months, 1 week ago (June 28, 2025, 12:01 p.m.)
Issued	17 years, 8 months ago (Dec. 19, 2007)
Published	17 years, 8 months ago (Dec. 19, 2007)
Published Online	17 years, 8 months ago (Dec. 19, 2007)
Published Print	17 years, 8 months ago (Jan. 1, 2008)

Funders 0

None

BibTeX

@article{Kang_2007, title={Proteomic analysis of the proteins regulated by HrpB from the plant pathogenic bacterium Burkholderia glumae}, volume={8}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200700244}, DOI={10.1002/pmic.200700244}, number={1}, journal={PROTEOMICS}, publisher={Wiley}, author={Kang, Yongsung and Kim, Jinwoo and Kim, Suhyun and Kim, Hongsup and Lim, Jae Yun and Kim, Minkyun and Kwak, Jangyul and Moon, Jae Sun and Hwang, Ingyu}, year={2007}, month=dec, pages={106–121} }

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