DOI: 10.1002/pmic.200400919. A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells

A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells

10.1002/pmic.200400919

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractThe tandem affinity purification (TAP) tag technique has been used with success to identify under nondenaturing conditions protein complexes in yeast. The technique can be used in mammalian cells, but we found that the original technique does not yield enough recovery for the identification of proteins when mammalian cells growing in monolayer have to be used. We present here a modified TAP tag technique that allows sufficient recovery of proteins from mouse fibroblasts growing in monolayer cultures. The recovery allows protein identification by mass spectrometry.

Bibliography

Drakas, R., Prisco, M., & Baserga, R. (2005). A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells. PROTEOMICS, 5(1), 132â137. Portico.

Authors 3

Robert Drakas (first)
Marco Prisco (additional)
Renato Baserga (additional)

References 31 Referenced 52

Dates

Type	When
Created	20 years, 8 months ago (Dec. 15, 2004, 8:47 a.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 9:37 p.m.)
Indexed	1 year, 2 months ago (June 6, 2024, 5:15 p.m.)
Issued	20 years, 8 months ago (Jan. 1, 2005)
Published	20 years, 8 months ago (Jan. 1, 2005)
Published Online	20 years, 7 months ago (Jan. 25, 2005)
Published Print	20 years, 8 months ago (Jan. 1, 2005)

Funders 0

None

BibTeX

@article{Drakas_2005, title={A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells}, volume={5}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200400919}, DOI={10.1002/pmic.200400919}, number={1}, journal={PROTEOMICS}, publisher={Wiley}, author={Drakas, Robert and Prisco, Marco and Baserga, Renato}, year={2005}, month=jan, pages={132–137} }

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