DOI: 10.1002/pmic.200300530. Profiling of cytokine expression by biotin‐labeled‐based protein arrays

Profiling of cytokine expression by biotin‐labeled‐based protein arrays

10.1002/pmic.200300530

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractGlobal analysis of protein expression holds great promise in basic research and patient care. Previously we demonstrated that multiple cytokines could be detected simultaneously using an enzyme‐linked immunosorbent assay protein array system with high sensitivity and specificity. In this paper, we described a biotin‐labeled‐based protein array system to detect multiple cytokines simultaneously from biological samples. In this new approach, proteins from a variety of biological sources are labeled with biotin. The biotin‐labeled proteins are then incubated with antibody chips. Targeted proteins are captured by the array antibodies spotted on the antibody chips. The presence of targeted proteins is detected using Cy3‐ or Cy5‐conjugated streptavidin and signals are imaged by laser scanner. The system also can be easily adapted to a two‐color binding assay, allowing measurement of the levels of proteins in a test sample with respect to a reference sample at the same chip. To demonstrate its potential applications, we applied this technology to profile human cytokines, chemokines, growth factors, angiogenic factors and proteases in estrogen receptor (ER)+ and ER− cells. These results suggest that biotin‐labeled‐based antibody chip technology can provide a practical and powerful means of profiling hundreds or thousands of proteins for research and clinical purposes.

Bibliography

Lin, Y., Huang, R., Chen, L., Lisoukov, H., Lu, Z., Li, S., Wang, C. C., & Huang, R. (2003). Profiling of cytokine expression by biotinâlabeledâbased protein arrays. PROTEOMICS, 3(9), 1750â1757. Portico.

Authors 8

Ying Lin (first)
Ruochun Huang (additional)
Li‐Pai Chen (additional)
Henry Lisoukov (additional)
Zhen‐Hai Lu (additional)
Shiyong Li (additional)
Cheng C. Wang (additional)
Ruo‐Pan Huang (additional)

References 20 Referenced 47

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Dates

Type	When
Created	21 years, 11 months ago (Sept. 10, 2003, 9:33 a.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 10:38 p.m.)
Indexed	1 year, 3 months ago (June 6, 2024, 5:14 p.m.)
Issued	22 years ago (Sept. 1, 2003)
Published	22 years ago (Sept. 1, 2003)
Published Online	21 years, 11 months ago (Sept. 9, 2003)
Published Print	22 years ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Lin_2003, title={Profiling of cytokine expression by biotin‐labeled‐based protein arrays}, volume={3}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200300530}, DOI={10.1002/pmic.200300530}, number={9}, journal={PROTEOMICS}, publisher={Wiley}, author={Lin, Ying and Huang, Ruochun and Chen, Li‐Pai and Lisoukov, Henry and Lu, Zhen‐Hai and Li, Shiyong and Wang, Cheng C. and Huang, Ruo‐Pan}, year={2003}, month=sep, pages={1750–1757} }

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