DOI: 10.1002/pmic.200300495. Mining biomarkers in human sera using proteomic tools

Mining biomarkers in human sera using proteomic tools

10.1002/pmic.200300495

Crossref journal-article

Wiley

PROTEOMICS (311)

Abstract

AbstractOne of the major difficulties in mining low abundance biomarkers from serum or plasma is due to the fact that a small number of proteins such as albumin, α2‐macroglobulin, transferrin, and immunoglobulins, may represent as much as 80% of the total serum protein. The large quantity of these proteins makes it difficult to identify low abundance proteins in serum using traditional 2‐dimensional electrophoresis. We recently used a combination of multidimensional liquid chromatography and gel electrophoresis coupled to matrix‐assisted laser desorption/ionization‐quadrupole‐time of flight and Ion Trap liquid chromatography‐tandem mass spectrometry to identify protein markers in sera of Alzheimer's disease (AD), insulin resistance/type‐2 diabetes (IR/D2), and congestive heart failure (CHF) patients. We identified 8 proteins that exhibit higher levels in control sera and 36 proteins that exhibit higher levels in disease sera. For example, haptoglobin and hemoglobin are elevated in sera of AD, IR/D2, and CHF patients. The levels of several other proteins including fibrinogen and its fragments, alpha 2‐macroglobulin, transthyretin, pro‐platelet basic protein, protease inhibitors clade A and C, as well as proteins involved in the classical complement pathway such as complement C3, C4, and C1 inhibitor, were found to differ between IR/D2 and control sera. The sera levels of proteins, such as the 10 kDa subunit of vitronectin, alpha 1‐acid glycoprotein, apolipoprotein B100, fragment of factor H, and histidine‐rich glycoprotein were observed to be different between AD and controls. The differences observed in these biomarker candidates were confirmed by Western blot and the enzyme‐linked immunosorbent assay. The biological meaning of the proteomic changes in the disease states and the potential use of these changes as diagnostic tools or for therapeutic intervention will be discussed.

Bibliography

Zhang, R., Barker, L., Pinchev, D., Marshall, J., Rasamoelisolo, M., Smith, C., Kupchak, P., Kireeva, I., Ingratta, L., & Jackowski, G. (2004). Mining biomarkers in human sera using proteomic tools. PROTEOMICS, 4(1), 244â256. Portico.

Authors 10

Rulin Zhang (first)
Lisa Barker (additional)
Deborah Pinchev (additional)
John Marshall (additional)
Michèle Rasamoelisolo (additional)
Chris Smith (additional)
Peter Kupchak (additional)
Inga Kireeva (additional)
Leslee Ingratta (additional)
George Jackowski (additional)

References 34 Referenced 143

{'key': 'e_1_2_1_2_2', 'first-page': '10.21.1', 'volume-title': 'Current Protocols in Molecular Biology', 'author': 'Carr S. A.', 'year': '1997'} / Current Protocols in Molecular Biology by Carr S. A. (1997)
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Dates

Type	When
Created	21 years, 7 months ago (Jan. 12, 2004, 11:01 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 10:59 a.m.)
Indexed	11 months, 4 weeks ago (Sept. 5, 2024, 2:52 p.m.)
Issued	21 years, 8 months ago (Jan. 1, 2004)
Published	21 years, 8 months ago (Jan. 1, 2004)
Published Online	21 years, 7 months ago (Jan. 12, 2004)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Zhang_2004, title={Mining biomarkers in human sera using proteomic tools}, volume={4}, ISSN={1615-9861}, url={http://dx.doi.org/10.1002/pmic.200300495}, DOI={10.1002/pmic.200300495}, number={1}, journal={PROTEOMICS}, publisher={Wiley}, author={Zhang, Rulin and Barker, Lisa and Pinchev, Deborah and Marshall, John and Rasamoelisolo, Michèle and Smith, Chris and Kupchak, Peter and Kireeva, Inga and Ingratta, Leslee and Jackowski, George}, year={2004}, month=jan, pages={244–256} }

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