DOI: 10.1002/pd.1970150808. The effect of thermal instability of intact human chorionic gonadotropin (ihcg) on the application of its free β‐subunit (free βhcg) as a serum marker in down syndrome screening

The effect of thermal instability of intact human chorionic gonadotropin (ihcg) on the application of its free β‐subunit (free βhcg) as a serum marker in down syndrome screening

10.1002/pd.1970150808

Crossref journal-article

Wiley

Prenatal Diagnosis (311)

Abstract

AbstractThe kinetics of free βhCG concentrations were measured in 30 maternal whole blood samples from second‐trimester pregnancies during 72 h incubation at 3, 20, and 30°C. Dissociation of intact hCG (ihCG) was undetectable at 3°C and produced a more than 20 per cent increase of free βhCG at 20°C and a more than 100 per cent increase at 30°C. hCG dissociation at 30°C was not reduced by a protease inhibitor (sodium iodoacetate) and also occurred in purified hCG dissolved in a protease‐free incubation medium. These results were reproduced under conditions of sample transport by post at different environmental temperatures. In conclusion, reliable free βhCG assessment requires that the specimen be kept cool from vene puncture until assay or completely other transport strategies have to be considered. Evaluation of free βhCG as an effective marker in prenatal Down syndrome screening must be reconsidered from this aspect.

Bibliography

Sancken, U., & Bahner, D. (1995). The effect of thermal instability of intact human chorionic gonadotropin (ihcg) on the application of its free Î²âsubunit (free Î²hcg) as a serum marker in down syndrome screening. Prenatal Diagnosis, 15(8), 731â738. Portico.

Authors 2

U. Sancken (first)
D. Bahner (additional)

References 17 Referenced 17

{'key': 'e_1_2_1_2_1', 'first-page': '297', 'article-title': 'Chemistry of human choriogonadotropin', 'volume': '45', 'author': 'Birken S.', 'year': '1984', 'journal-title': 'Ann. Endocrinol.'} / Ann. Endocrinol. / Chemistry of human choriogonadotropin by Birken S. (1984)
10.1177/000456329403100119
10.1093/clinchem/37.6.779 / Clin. Chem. / Measurement of choriogonadotropin free β‐subunit: an alternative to choriogonadotropin in screening for fetal Down's syndrome? by Knight G. J. (1991)
10.1002/pd.1970140311
10.1016/0002-9378(90)90700-H
10.1002/pd.1970130704
10.1002/pd.1970140204
10.1016/0002-9378(84)90530-1
10.1146/annurev.bi.50.070181.002341
10.1002/pd.1970120404
10.1093/clinchem/37.6.809 / Clin. Chem. / Evaluation of an assay of the free β‐subunit of choriogonadotropin and its potential value in screening for Down's syndrome by Spencer K. (1991)
10.1177/000456329202900504
10.1016/0140-6736(92)92073-O
10.1093/clinchem/39.6.1064 / Clin. Chem. / Stability of intact chorionic gonadotropin (hCG) in serum, liquid whole blood, and dried whole‐blood filter‐paper spots: impact on screening for Down syndrome by measurement of free βhCG subunit by Spencer K. (1993)
10.1177/000456329303000118
10.1111/j.1471-0528.1993.tb15307.x
Zimmermann R. Keller P. J. Huch A.(1993). Increased maternal serum free β‐human chorionic gonadotropin concentrations in Down pregnancies: an artefactual finding? Fourth Conference of Endocrinology and Metabolism in Human Reproduction Abstract No. 53.

Dates

Type	When
Created	18 years, 6 months ago (March 4, 2007, 6:11 a.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 12:20 a.m.)
Indexed	1 year, 3 months ago (May 31, 2024, 8:57 p.m.)
Issued	30 years, 1 month ago (Aug. 1, 1995)
Published	30 years, 1 month ago (Aug. 1, 1995)
Published Online	19 years, 8 months ago (Dec. 14, 2005)
Published Print	30 years, 1 month ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Sancken_1995, title={The effect of thermal instability of intact human chorionic gonadotropin (ihcg) on the application of its free β‐subunit (free βhcg) as a serum marker in down syndrome screening}, volume={15}, ISSN={1097-0223}, url={http://dx.doi.org/10.1002/pd.1970150808}, DOI={10.1002/pd.1970150808}, number={8}, journal={Prenatal Diagnosis}, publisher={Wiley}, author={Sancken, U. and Bahner, D.}, year={1995}, month=aug, pages={731–738} }

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