DOI: 10.1002/path.1711760215. Microwave antigen retrieval for immunocytochemistry on formalin‐fixed, paraffin‐embedded post‐mortem CNS tissue

Microwave antigen retrieval for immunocytochemistry on formalin‐fixed, paraffin‐embedded post‐mortem CNS tissue

10.1002/path.1711760215

Crossref journal-article

Wiley

The Journal of Pathology (311)

Abstract

AbstractMicrowave antigen retrieval methods were assessed for a panel of antibodies on formalin‐fixed, paraffin‐embedded sections from a range of human central nervous system (CNS) tissues taken at post‐mortem. The immunoreactivity of a number of antigens (synaptophysin, PGP9.5, GFAP, carbonic anhydrase II, CD68, ferritin, HLA‐DR, αβ‐crystallin, mealses and herpes viruses) was markedly enhanced by this procedure compared with other methods of antigen unmasking, such as trypsinization. Enhancement was noted both by an increased number of positive cells and by the intensity of reactions within individual cells and their processes. Furthermore, the microwave method produced uniform immunostaining over large surface area sections with no loss of morphological detail. Large cryostat sections of CNS tissue can be difficult to obtain with good morphology. The ability to retrieve a wide range of antigen in formalin‐fixed, paraffin‐embedded CNS tissue will greatly increase the range of investigations that can be carried out on this type of stored material.

Bibliography

McQuaid, S., McConnell, R., McMahon, J., & Herron, B. (1995). Microwave antigen retrieval for immunocytochemistry on formalinâfixed, paraffinâembedded postâmortem CNS tissue. The Journal of Pathology, 176(2), 207â216. Portico.

Authors 4

Stephen McQuaid (first)
Robert McConnell (additional)
Jill McMahon (additional)
Brian Herron (additional)

References 19 Referenced 42

{'key': 'e_1_2_1_2_2', 'first-page': '156', 'volume-title': 'Encyclopedia of Neuroscience', 'author': 'Tourtellotte WW', 'year': '1987'} / Encyclopedia of Neuroscience by Tourtellotte WW (1987)
{'key': 'e_1_2_1_3_2', 'first-page': '403', 'volume-title': 'Molecular Anatomy', 'author': 'Swaab DF', 'year': '1989'} / Molecular Anatomy by Swaab DF (1989)
10.1016/0022-1759(77)90027-8
{'key': 'e_1_2_1_5_2', 'first-page': '239', 'volume-title': 'Techniques in Immunocytochemistry', 'author': 'Finley JCW', 'year': '1982'} / Techniques in Immunocytochemistry by Finley JCW (1982)
10.1177/40.6.1588025
10.1177/030098588902600406
{'key': 'e_1_2_1_8_2', 'first-page': '230', 'article-title': 'Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloidosis', 'volume': '57', 'author': 'Kitamoto T', 'year': '1987', 'journal-title': 'Lab Invest'} / Lab Invest / Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloidosis by Kitamoto T (1987)
10.1177/39.6.1709656
10.1002/path.1711710205
{'key': 'e_1_2_1_11_2', 'first-page': '381', 'article-title': 'Detection of p53 overexpression in routinely paraffin embedded tissue of human carcinomas using a novel target unmasking fluid (TUF)', 'volume': '142', 'author': 'Van Den Berg F', 'year': '1993', 'journal-title': 'Am J Pathol'} / Am J Pathol / Detection of p53 overexpression in routinely paraffin embedded tissue of human carcinomas using a novel target unmasking fluid (TUF) by Van Den Berg F (1993)
10.1136/jcp.40.10.1256
{'key': 'e_1_2_1_13_2', 'volume-title': 'Theory and Practice of Histological Techniques', 'author': 'Bancroft JD', 'year': '1982'} / Theory and Practice of Histological Techniques by Bancroft JD (1982)
{'key': 'e_1_2_1_14_2', 'first-page': '143', 'article-title': 'Optimizing keratin and vimentin retrieval in formalin‐fixed, paraffin‐embedded tissue with the use of heat and metal salts', 'volume': '1', 'author': 'Suurmeijer AJH', 'year': '1993', 'journal-title': 'Appl Immunohistochem'} / Appl Immunohistochem / Optimizing keratin and vimentin retrieval in formalin‐fixed, paraffin‐embedded tissue with the use of heat and metal salts by Suurmeijer AJH (1993)
10.1016/0165-5728(87)90082-8
10.1016/S0165-5728(85)80043-6
10.1111/j.1365-2990.1993.tb00431.x
{'key': 'e_1_2_1_18_2', 'first-page': '693', 'article-title': 'Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin‐fixed normal and diseased brain tissues', 'volume': '64', 'author': 'Shin R‐W', 'year': '1991', 'journal-title': 'Lab Invest'} / Lab Invest / Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin‐fixed normal and diseased brain tissues by Shin R‐W (1991)
{'key': 'e_1_2_1_19_2', 'first-page': '107A', 'article-title': 'A novel method of enhancing immunohistochemical staining using brief high temperature heat denaturation', 'volume': '172', 'author': 'Norton AJ', 'year': '1994', 'journal-title': 'J Pathol'} / J Pathol / A novel method of enhancing immunohistochemical staining using brief high temperature heat denaturation by Norton AJ (1994)
10.1016/0166-0934(93)90009-G

Dates

Type	When
Created	20 years, 2 months ago (June 22, 2005, 1:17 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 9:36 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 7, 2024, 11:29 a.m.)
Issued	30 years, 2 months ago (June 1, 1995)
Published	30 years, 2 months ago (June 1, 1995)
Published Online	20 years, 2 months ago (June 15, 2005)
Published Print	30 years, 2 months ago (June 1, 1995)

Funders 0

None

BibTeX

@article{McQuaid_1995, title={Microwave antigen retrieval for immunocytochemistry on formalin‐fixed, paraffin‐embedded post‐mortem CNS tissue}, volume={176}, ISSN={1096-9896}, url={http://dx.doi.org/10.1002/path.1711760215}, DOI={10.1002/path.1711760215}, number={2}, journal={The Journal of Pathology}, publisher={Wiley}, author={McQuaid, Stephen and McConnell, Robert and McMahon, Jill and Herron, Brian}, year={1995}, month=jun, pages={207–216} }

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