DOI: 10.1002/path.1711720104. An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms

An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms

10.1002/path.1711720104

Crossref journal-article

Wiley

The Journal of Pathology (311)

Abstract

AbstractImmunohistochemical detection of intranuclear p53 gene product may indicate mutation of the p53 suppressor gene on chromosome 17p. We used six commercially available antibodies for p53 immunohistochemistry on 19 archival colorectal neoplasms and compared the results with the mutation status of the p53 gene and 17p allelic deletion status. By Friedman's ranking analysis, use of mouse monoclonal antibody DO7 with Target Unmasking Fluid (TUF) for antigen retrieval was the most sensitive and specific procedure (P<0·0001). Six of 7 cases with high expression (p53 Labeling Index >30 per cent using a CAS 200 image analyser) had p53 mutation. Of seven tumours without expression (LI < 1 per cent), six had no mutation and one had a truncating mutation which prohibited nuclear localization of gene product. The low expression group (1 per cent < LI <30 per cent, n = 5) consisted of three tumours without and two tumours with mutation. The sensitivity of high expression with the DO7–TUF method for p53 gene mutation was 67 per cent with specificity of 90 per cent, predictive value of a positive of 86 per cent, predictive value of a negative of 75 per cent, and efficiency of 79 per cent. This study suggests that immunohistochemistry is valuable for assessing p53 gene mutations in colorectal neoplasms, but further study is needed to elucidate the precise link between immunohistochemistry and molecular genetic alterations.

Bibliography

Baas, I. O., Mulder, J. R., Offerhaus, G. J. A., Vogelstein, B., & Hamilton, S. R. (1994). An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms. The Journal of Pathology, 172(1), 5â12. Portico.

Authors 5

Inge O. Baas (first)
Jan‐Willem R. Mulder (additional)
G. Johan A. Offerhaus (additional)
Bert Vogelstein (additional)
Stanley R. Hamilton (additional)

References 37 Referenced 383

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{'key': 'e_1_2_1_5_2', 'first-page': '59', 'article-title': 'The p53 tumor suppressor gene and product', 'volume': '12', 'author': 'Levine AJ', 'year': '1992', 'journal-title': 'Cancer Surv'} / Cancer Surv / The p53 tumor suppressor gene and product by Levine AJ (1992)
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{'key': 'e_1_2_1_37_2', 'first-page': '3465', 'article-title': 'Accumulation of wild type p53 protein in human astrocytomas', 'volume': '53', 'author': 'Rubio M‐P', 'year': '1993', 'journal-title': 'Cancer Res'} / Cancer Res / Accumulation of wild type p53 protein in human astrocytomas by Rubio M‐P (1993)
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Dates

Type	When
Created	20 years, 2 months ago (June 22, 2005, 12:26 p.m.)
Deposited	1 year, 11 months ago (Sept. 10, 2023, 1:53 p.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:47 a.m.)
Issued	31 years, 8 months ago (Jan. 1, 1994)
Published	31 years, 8 months ago (Jan. 1, 1994)
Published Online	20 years, 2 months ago (June 15, 2005)
Published Print	31 years, 8 months ago (Jan. 1, 1994)

Funders 5

The Netherlands Digestive Disease Foundation
Awards1
1. WS 92.33
STIR & American Connection, State University of Leiden
The Clayton Fund
The McAshan Fund
National Cancer Institute, National Institute Health
Awards1
1. R01 CA47527

BibTeX

@article{Baas_1994, title={An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms}, volume={172}, ISSN={1096-9896}, url={http://dx.doi.org/10.1002/path.1711720104}, DOI={10.1002/path.1711720104}, number={1}, journal={The Journal of Pathology}, publisher={Wiley}, author={Baas, Inge O. and Mulder, Jan‐Willem R. and Offerhaus, G. Johan A. and Vogelstein, Bert and Hamilton, Stanley R.}, year={1994}, month=jan, pages={5–12} }

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