DOI: 10.1002/path.1711420112. Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis

Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis

10.1002/path.1711420112

Crossref journal-article

Wiley

The Journal of Pathology (311)

Abstract

AbstractIn glucocorticoid‐treated rat thymocytes and the murine lymphoid cell lines L5178 and S49 the morphology of apoptosis is associated with chromatin cleavage. The cleavage is at internucleosomal sites, apparently through activation of an endogenous endonuclease. In variants of the cell lines selected for resistance to glucocorticoid, neither apoptosis nor chromatin cleavage were observed after steroid treatment, and steroid receptors were undetectable. In thymocytes, both the morphological changes of apoptosis and chromatin cleavage were inhibited by cycloheximide and actinomycin D. The calcium–magnesium ionophore A23187 induced apoptosis and chromatin cleavage in thymocytes, and these effects were also inhibited by cycloheximide. The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments. They also provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.

Bibliography

Wyllie, A. H., Morris, R. G., Smith, A. L., & Dunlop, D. (1984). Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis. The Journal of Pathology, 142(1), 67â77. Portico.

Authors 4

A. H. Wyllie (first)
R. G. Morris (additional)
A. L. Smith (additional)
D. Dunlop (additional)

References 47 Referenced 1,129

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Dates

Type	When
Created	20 years, 2 months ago (June 21, 2005, 2 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 8:30 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 5, 2025, 9:10 a.m.)
Issued	41 years, 7 months ago (Jan. 1, 1984)
Published	41 years, 7 months ago (Jan. 1, 1984)
Published Online	20 years, 2 months ago (June 15, 2005)
Published Print	41 years, 7 months ago (Jan. 1, 1984)

Funders 0

None

BibTeX

@article{Wyllie_1984, title={Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis}, volume={142}, ISSN={1096-9896}, url={http://dx.doi.org/10.1002/path.1711420112}, DOI={10.1002/path.1711420112}, number={1}, journal={The Journal of Pathology}, publisher={Wiley}, author={Wyllie, A. H. and Morris, R. G. and Smith, A. L. and Dunlop, D.}, year={1984}, month=jan, pages={67–77} }

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