DOI: 10.1002/path.1376. Raman spectroscopy, a potential tool for the objective identification and classification of neoplasia in Barrett's oesophagus

Raman spectroscopy, a potential tool for the objective identification and classification of neoplasia in Barrett's oesophagus

10.1002/path.1376

Crossref journal-article

Wiley

The Journal of Pathology (311)

Abstract

AbstractHistopathology remains the gold standard technique for the diagnosis of intraepithelial neoplasia (dysplasia) in Barrett's oesophagus, but it is highly subjective and relies on blind biopsy targeting. The aim of this study was to evaluate Raman spectroscopy, a rapid, non‐invasive, molecular, specific analytical technique, for the objective identification and classification of Barrett's neoplasia in vitro. A secondary objective was to demonstrate the need for a rigorous gold standard in the development of new diagnostic techniques. Forty‐four patients with a mean age of 69 years (range 34–89 years) undergoing surveillance for Barrett's oesophagus were included in the study. Three consultant pathologists independently assessed snap‐frozen oesophageal biopsy specimens. Raman spectra were measured on 87 histopathologically homogeneous samples. Spectral classification models were developed using multivariate analysis for the prediction of pathology. Histopathology and Raman classification results were compared. Raman spectral prediction with a consensus pathology classification model gave sensitivities between 73% and 100% and specificities of 90–100%. A high level of agreement (κ = 0.89) was demonstrated between the three‐subset biopsy targeting model and consensus pathology opinion. This compares favourably with the agreement measured between an independent pathologist and the consensus pathology opinion for the same spectra (κ = 0.76). Raman spectroscopy appears to provide a highly sensitive and specific technique for the identification and classification of neoplasia in Barrett's oesophagus. Copyright © 2003 John Wiley & Sons, Ltd.

Bibliography

Kendall, C., Stone, N., Shepherd, N., Geboes, K., Warren, B., Bennett, R., & Barr, H. (2003). Raman spectroscopy, a potential tool for the objective identification and classification of neoplasia in Barrettâs oesophagus. The Journal of Pathology, 200(5), 602â609. Portico.

Authors 7

Catherine Kendall (first)
Nicholas Stone (additional)
Neil Shepherd (additional)
Karel Geboes (additional)
Bryan Warren (additional)
Robert Bennett (additional)
Hugh Barr (additional)

References 40 Referenced 198

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Dates

Type	When
Created	22 years, 1 month ago (July 29, 2003, 6:14 a.m.)
Deposited	1 year, 9 months ago (Nov. 17, 2023, 10:16 p.m.)
Indexed	1 week, 3 days ago (Aug. 23, 2025, 12:59 a.m.)
Issued	22 years, 5 months ago (March 31, 2003)
Published	22 years, 5 months ago (March 31, 2003)
Published Online	22 years, 5 months ago (March 31, 2003)
Published Print	22 years, 1 month ago (Aug. 1, 2003)

Funders 0

None

BibTeX

@article{Kendall_2003, title={Raman spectroscopy, a potential tool for the objective identification and classification of neoplasia in Barrett’s oesophagus}, volume={200}, ISSN={1096-9896}, url={http://dx.doi.org/10.1002/path.1376}, DOI={10.1002/path.1376}, number={5}, journal={The Journal of Pathology}, publisher={Wiley}, author={Kendall, Catherine and Stone, Nicholas and Shepherd, Neil and Geboes, Karel and Warren, Bryan and Bennett, Robert and Barr, Hugh}, year={2003}, month=mar, pages={602–609} }

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