DOI: 10.1002/neu.480140602. Evidence for a presynaptic blockage of transmission in a temperature‐sensitive mutant of <i>Drosophila</i>

Evidence for a presynaptic blockage of transmission in a temperature‐sensitive mutant of Drosophila

10.1002/neu.480140602

Crossref journal-article

Wiley

Journal of Neurobiology (311)

Abstract

AbstractIn the temperature sensitive mutant of Drosophila, shibirets1 (shi), synaptic transmission in the dorsal longitudinal flight muscles (DLM) is normal at 19°C, but is diminished progressively as the temperature is raised, and is blocked at 29°C. The purpose of this paper is to determine whether this defect is located presynaptically, postsynaptically, or both. It is demonstrated here that the postsynaptic sensitivity to L‐glutamate, the putative transmitter for this synapse, is not decreased at 29°C. Furthermore, studies conducted with genetic mosaics of this mutant show that transmission is blocked when a mutant motor neuron synapses on a wild‐type muscle fiber, but is not blocked when a wild‐type motor neuron synapses on a mutant muscle fiber. Thus, the shi phenotype (temperature dependent transmission block) correlates with a shi motor neuron, not with a shi muscle fiber. The data, therefore, suggest that the defect is not postsynaptic, but presynaptic.

Bibliography

Koenig, J. H., & Ikeda, K. (1983). Evidence for a presynaptic blockage of transmission in a temperatureâsensitive mutant of Drosophila. Journal of Neurobiology, 14(6), 411â419. Portico.

Authors 2

J. H. Koenig (first)
Kazuo Ikeda (additional)

References 23 Referenced 34

10.1002/cne.901770410
10.1113/jphysiol.1978.sp012226
{'key': 'e_1_2_1_4_1', 'first-page': '105', 'article-title': 'Desensitization and potentiation during glutamate application to locust skeletal muscle', 'volume': '59', 'author': 'Daoud M. A. R.', 'year': '1978', 'journal-title': 'Comp. Biochem. Physiol.'} / Comp. Biochem. Physiol. / Desensitization and potentiation during glutamate application to locust skeletal muscle by Daoud M. A. R. (1978)
10.1007/BF00267238
{'key': 'e_1_2_1_6_1', 'first-page': '728', 'article-title': 'Three temperature‐sensitive loci causing paralysis in Drosophila melanogaster', 'volume': '14', 'author': 'Hall L.', 'year': '1972', 'journal-title': 'Can. J. Genet. Cytol.'} / Can. J. Genet. Cytol. / Three temperature‐sensitive loci causing paralysis in Drosophila melanogaster by Hall L. (1972)
{'key': 'e_1_2_1_7_1', 'first-page': '369', 'volume-title': 'Genetics and Biology of Drosophila', 'author': 'Ikeda K.', 'year': '1980'} / Genetics and Biology of Drosophila by Ikeda K. (1980)
10.1073/pnas.67.3.1480
10.1038/259489a0
10.1007/BF01205020
{'key': 'e_1_2_1_11_1', 'first-page': '737', 'article-title': 'Morphological identification of the 5 motor neurons innervating the dorsal longitudinal flight muscle of Drosophila', 'volume': '8', 'author': 'Ikeda K.', 'year': '1982', 'journal-title': 'Soc. Neurosci. Abstracts'} / Soc. Neurosci. Abstracts / Morphological identification of the 5 motor neurons innervating the dorsal longitudinal flight muscle of Drosophila by Ikeda K. (1982)
10.1073/pnas.71.12.4906
10.1002/neu.480110510
Koenig J. H. Saito K. andIkeda K.(1983). Reversible control of synaptic transmission in a single‐gene mutant ofDrosophila melanogaster.J. Cell Biol. in press. (10.1083/jcb.96.6.1517)
Kosaka T. andIkeda K.(1983a). Possible temperature‐dependent blockage of synaptic vesicle recycling induced by a single gene mutation inDrosophila.J. Neurobiol. in press. (10.1002/neu.480140305)
Kosaka T. andIkeda K.(1983b). Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature‐sensitive mutant ofDrosophila melanogaster.J. Cell Biol. in press. (10.1083/jcb.97.2.499)
10.1016/0010-4035(73)90045-1
{'key': 'e_1_2_1_18_1', 'volume-title': 'Genetic variations of Drosophila melanogaster', 'author': 'Lindsley D. L.', 'year': '1968'} / Genetic variations of Drosophila melanogaster by Lindsley D. L. (1968)
{'key': 'e_1_2_1_19_1', 'first-page': '106', 'article-title': 'Untersuchunge über Anatomie und Mechanik der Flugorgane and der Stubenfliege', 'volume': '1', 'author': 'Mihályi F.', 'year': '1936', 'journal-title': 'Ung. Biol. Forsch.'} / Ung. Biol. Forsch. / Untersuchunge über Anatomie und Mechanik der Flugorgane and der Stubenfliege by Mihályi F. (1936)
10.1016/0022-1910(75)90227-9
10.1083/jcb.81.3.520
10.1038/273156a0
{'key': 'e_1_2_1_23_1', 'first-page': '1', 'article-title': 'An electron microscopical study of the innervation and sarcoplasmic reticulum of the fibrillar flight muscle of Drosophila melanogaster', 'volume': '105', 'author': 'Shafiq S. A.', 'year': '1964', 'journal-title': 'Quar. J. Micro. Sci.'} / Quar. J. Micro. Sci. / An electron microscopical study of the innervation and sarcoplasmic reticulum of the fibrillar flight muscle of Drosophila melanogaster by Shafiq S. A. (1964)
10.1073/pnas.73.9.3253

Dates

Type	When
Created	20 years, 8 months ago (Dec. 31, 2004, 11:19 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 7:20 p.m.)
Indexed	1 year, 4 months ago (May 6, 2024, 1:21 p.m.)
Issued	41 years, 10 months ago (Nov. 1, 1983)
Published	41 years, 10 months ago (Nov. 1, 1983)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	41 years, 10 months ago (Nov. 1, 1983)

Funders 0

None

BibTeX

@article{Koenig_1983, title={Evidence for a presynaptic blockage of transmission in a temperature‐sensitive mutant of Drosophila}, volume={14}, ISSN={1097-4695}, url={http://dx.doi.org/10.1002/neu.480140602}, DOI={10.1002/neu.480140602}, number={6}, journal={Journal of Neurobiology}, publisher={Wiley}, author={Koenig, J. H. and Ikeda, Kazuo}, year={1983}, month=nov, pages={411–419} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        6
      ]
    ],
    "date-time": "2024-05-06T17:21:01Z",
    "timestamp": 1715016061093
  },
  "reference-count": 23,
  "publisher": "Wiley",
  "issue": "6",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            11
          ]
        ],
        "date-time": "2004-10-11T00:00:00Z",
        "timestamp": 1097452800000
      },
      "content-version": "vor",
      "delay-in-days": 7650,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1983,
        11
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>In the temperature sensitive mutant of <jats:italic>Drosophila, shibire</jats:italic><jats:sup>ts1</jats:sup> (<jats:italic>shi</jats:italic>), synaptic transmission in the dorsal longitudinal flight muscles (DLM) is normal at 19\u00b0C, but is diminished progressively as the temperature is raised, and is blocked at 29\u00b0C. The purpose of this paper is to determine whether this defect is located presynaptically, postsynaptically, or both. It is demonstrated here that the postsynaptic sensitivity to L\u2010glutamate, the putative transmitter for this synapse, is not decreased at 29\u00b0C. Furthermore, studies conducted with genetic mosaics of this mutant show that transmission is blocked when a mutant motor neuron synapses on a wild\u2010type muscle fiber, but is not blocked when a wild\u2010type motor neuron synapses on a mutant muscle fiber. Thus, the <jats:italic>shi</jats:italic> phenotype (temperature dependent transmission block) correlates with a <jats:italic>shi</jats:italic> motor neuron, not with a <jats:italic>shi</jats:italic> muscle fiber. The data, therefore, suggest that the defect is not postsynaptic, but presynaptic.</jats:p>",
  "DOI": "10.1002/neu.480140602",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        1
      ]
    ],
    "date-time": "2005-01-01T04:19:56Z",
    "timestamp": 1104553196000
  },
  "page": "411-419",
  "source": "Crossref",
  "is-referenced-by-count": 34,
  "title": "Evidence for a presynaptic blockage of transmission in a temperature\u2010sensitive mutant of <i>Drosophila</i>",
  "prefix": "10.1002",
  "volume": "14",
  "author": [
    {
      "given": "J. H.",
      "family": "Koenig",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Kazuo",
      "family": "Ikeda",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.901770410"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1113/jphysiol.1978.sp012226"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "105",
      "article-title": "Desensitization and potentiation during glutamate application to locust skeletal muscle",
      "volume": "59",
      "author": "Daoud M. A. R.",
      "year": "1978",
      "journal-title": "Comp. Biochem. Physiol."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00267238"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "728",
      "article-title": "Three temperature\u2010sensitive loci causing paralysis in Drosophila melanogaster",
      "volume": "14",
      "author": "Hall L.",
      "year": "1972",
      "journal-title": "Can. J. Genet. Cytol."
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "369",
      "volume-title": "Genetics and Biology of Drosophila",
      "author": "Ikeda K.",
      "year": "1980"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.67.3.1480"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/259489a0"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01205020"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "737",
      "article-title": "Morphological identification of the 5 motor neurons innervating the dorsal longitudinal flight muscle of Drosophila",
      "volume": "8",
      "author": "Ikeda K.",
      "year": "1982",
      "journal-title": "Soc. Neurosci. Abstracts"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.71.12.4906"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/neu.480110510"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Koenig J. H. Saito K. andIkeda K.(1983). Reversible control of synaptic transmission in a single\u2010gene mutant ofDrosophila melanogaster.J. Cell Biol. in press.",
      "DOI": "10.1083/jcb.96.6.1517"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Kosaka T. andIkeda K.(1983a). Possible temperature\u2010dependent blockage of synaptic vesicle recycling induced by a single gene mutation inDrosophila.J. Neurobiol. in press.",
      "DOI": "10.1002/neu.480140305"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Kosaka T. andIkeda K.(1983b). Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature\u2010sensitive mutant ofDrosophila melanogaster.J. Cell Biol. in press.",
      "DOI": "10.1083/jcb.97.2.499"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0010-4035(73)90045-1"
    },
    {
      "key": "e_1_2_1_18_1",
      "volume-title": "Genetic variations of Drosophila melanogaster",
      "author": "Lindsley D. L.",
      "year": "1968"
    },
    {
      "key": "e_1_2_1_19_1",
      "first-page": "106",
      "article-title": "Untersuchunge \u00fcber Anatomie und Mechanik der Flugorgane and der Stubenfliege",
      "volume": "1",
      "author": "Mih\u00e1lyi F.",
      "year": "1936",
      "journal-title": "Ung. Biol. Forsch."
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-1910(75)90227-9"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.81.3.520"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/273156a0"
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "1",
      "article-title": "An electron microscopical study of the innervation and sarcoplasmic reticulum of the fibrillar flight muscle of Drosophila melanogaster",
      "volume": "105",
      "author": "Shafiq S. A.",
      "year": "1964",
      "journal-title": "Quar. J. Micro. Sci."
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.73.9.3253"
    }
  ],
  "container-title": "Journal of Neurobiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fneu.480140602",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/neu.480140602",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        19
      ]
    ],
    "date-time": "2023-10-19T23:20:11Z",
    "timestamp": 1697757611000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/neu.480140602"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1983,
        11
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1983,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/neu.480140602"
  ],
  "URL": "http://dx.doi.org/10.1002/neu.480140602",
  "relation": {},
  "ISSN": [
    "0022-3034",
    "1097-4695"
  ],
  "subject": [],
  "container-title-short": "J. Neurobiol.",
  "published": {
    "date-parts": [
      [
        1983,
        11
      ]
    ]
  }
}