DOI: 10.1002/neu.480010109. Acetoxycycloheximide effect on synthesis and metabolism of glucosamine‐containing macromolecules in brain and in nerve endings

Acetoxycycloheximide effect on synthesis and metabolism of glucosamine‐containing macromolecules in brain and in nerve endings

10.1002/neu.480010109

Crossref journal-article

Wiley

Journal of Neurobiology (311)

Abstract

AbstractCarbohydrate‐containing macromolecules of brain were substantially labeled after intracerebral injection of C14‐glucosamine. The “half‐life” of the labeled macromolecules, as measured in these experiments, was about one week. Acetoxycycloheximide inhibited incorporation of C14‐glucosamine into these substances. This appears to be secondary to inhibition of synthesis of polypeptide chains which act as “acceptors” for carbohydrate residues. Acetoxycycloheximide inhibited incorporation of C14‐glucosamine (and its derivatives) into the soluble carbohydrate‐containing macromolecules of nerve endings far less than that of the other subcellular fractions of brain. This suggests that the polypeptide “acceptors” of carbohydrate residues at nerve endings are not synthesized locally but are transported to the nerve endings. Final incorporation of carbohydrates into carbohydrate‐containing macromolecules occurs at nerve endings. The “turnover” of these substances in nerve endings was found to be relatively rapid. Evidence suggesting reversible incorporation and removal of some carbohydrate residues of carbohydrate‐containing macromolecules of brain was found and the possible regulatory role of this process is considered.

Bibliography

Barondes, S. H., & Dutton, G. R. (1969). Acetoxycycloheximide effect on synthesis and metabolism of glucosamineâcontaining macromolecules in brain and in nerve endings. Journal of Neurobiology, 1(1), 99â110. Portico.

Authors 2

Samuel H. Barondes (first)
Gary R. Dutton (additional)

References 17 Referenced 50

10.1126/science.146.3645.779
10.1111/j.1471-4159.1968.tb11619.x
10.1111/j.1471-4159.1968.tb08970.x
10.1126/science.160.3827.556
{'key': 'e_1_2_1_6_1', 'volume-title': 'The Biochemistry of Memory', 'author': 'Bogoch S.', 'year': '1968'} / The Biochemistry of Memory by Bogoch S. (1968)
10.1016/0003-2697(60)90025-7
{'key': 'e_1_2_1_8_1', 'first-page': '230', 'article-title': 'Gangliosides and Cerebrosides turnover rates in rat brain (abstract)', 'volume': '23', 'author': 'Burton R. M.', 'year': '1964', 'journal-title': 'Federation Proc.'} / Federation Proc. / Gangliosides and Cerebrosides turnover rates in rat brain (abstract) by Burton R. M. (1964)
10.1126/science.161.3840.473
Den H. andKaufman B.(1968).Ganglioside and glycoprotein glycosyltransferase in synaptosomes (Abstract). Federation Proc. p.346.
10.1016/0022-5193(66)90179-2
10.1073/pnas.52.3.750
10.1111/j.1471-4159.1967.tb09550.x
10.1126/science.151.3713.988
{'issue': '10', 'key': 'e_1_2_1_15_1', 'first-page': '3157', 'article-title': 'The biosynthesis of glycoproteins. III. Glucosamine intermediate in plasma glycoprotein synthesis in livers of puromycin‐treated rates', 'volume': '239', 'author': 'Molar J.', 'year': '1964', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / The biosynthesis of glycoproteins. III. Glucosamine intermediate in plasma glycoprotein synthesis in livers of puromycin‐treated rates by Molar J. (1964)
Neuberger A. andMarshall R. D.(1966).Methods for the qualitative and quantitative analysis of the component sugars. In: Glycoproteins A. Gottschalk (Ed.) pp.190–234.
10.1083/jcb.32.1.27
10.1111/j.1471-4159.1965.tb04256.x

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 3:49 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 7:24 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 9:57 a.m.)
Issued	56 years, 8 months ago (Jan. 1, 1969)
Published	56 years, 8 months ago (Jan. 1, 1969)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	56 years, 8 months ago (Jan. 1, 1969)

Funders 0

None

BibTeX

@article{Barondes_1969, title={Acetoxycycloheximide effect on synthesis and metabolism of glucosamine‐containing macromolecules in brain and in nerve endings}, volume={1}, ISSN={1097-4695}, url={http://dx.doi.org/10.1002/neu.480010109}, DOI={10.1002/neu.480010109}, number={1}, journal={Journal of Neurobiology}, publisher={Wiley}, author={Barondes, Samuel H. and Dutton, Gary R.}, year={1969}, month=jan, pages={99–110} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T14:57:21Z",
    "timestamp": 1704985041795
  },
  "reference-count": 17,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            11
          ]
        ],
        "date-time": "2004-10-11T00:00:00Z",
        "timestamp": 1097452800000
      },
      "content-version": "vor",
      "delay-in-days": 13067,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1969,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Carbohydrate\u2010containing macromolecules of brain were substantially labeled after intracerebral injection of C<jats:sup>14</jats:sup>\u2010glucosamine. The \u201chalf\u2010life\u201d of the labeled macromolecules, as measured in these experiments, was about one week. Acetoxycycloheximide inhibited incorporation of C<jats:sup>14</jats:sup>\u2010glucosamine into these substances. This appears to be secondary to inhibition of synthesis of polypeptide chains which act as \u201cacceptors\u201d for carbohydrate residues. Acetoxycycloheximide inhibited incorporation of C<jats:sup>14</jats:sup>\u2010glucosamine (and its derivatives) into the soluble carbohydrate\u2010containing macromolecules of nerve endings far less than that of the other subcellular fractions of brain. This suggests that the polypeptide \u201cacceptors\u201d of carbohydrate residues at nerve endings are not synthesized locally but are transported to the nerve endings. Final incorporation of carbohydrates into carbohydrate\u2010containing macromolecules occurs at nerve endings. The \u201cturnover\u201d of these substances in nerve endings was found to be relatively rapid. Evidence suggesting reversible incorporation and removal of some carbohydrate residues of carbohydrate\u2010containing macromolecules of brain was found and the possible regulatory role of this process is considered.</jats:p>",
  "DOI": "10.1002/neu.480010109",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        1
      ]
    ],
    "date-time": "2005-01-01T08:49:16Z",
    "timestamp": 1104569356000
  },
  "page": "99-110",
  "source": "Crossref",
  "is-referenced-by-count": 50,
  "title": "Acetoxycycloheximide effect on synthesis and metabolism of glucosamine\u2010containing macromolecules in brain and in nerve endings",
  "prefix": "10.1002",
  "volume": "1",
  "author": [
    {
      "given": "Samuel H.",
      "family": "Barondes",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Gary R.",
      "family": "Dutton",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.146.3645.779"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1968.tb11619.x"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1968.tb08970.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.160.3827.556"
    },
    {
      "key": "e_1_2_1_6_1",
      "volume-title": "The Biochemistry of Memory",
      "author": "Bogoch S.",
      "year": "1968"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(60)90025-7"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "230",
      "article-title": "Gangliosides and Cerebrosides turnover rates in rat brain (abstract)",
      "volume": "23",
      "author": "Burton R. M.",
      "year": "1964",
      "journal-title": "Federation Proc."
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.161.3840.473"
    },
    {
      "key": "e_1_2_1_10_1",
      "unstructured": "Den H. andKaufman B.(1968).Ganglioside and glycoprotein glycosyltransferase in synaptosomes (Abstract). Federation Proc. p.346."
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-5193(66)90179-2"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.52.3.750"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1967.tb09550.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.151.3713.988"
    },
    {
      "issue": "10",
      "key": "e_1_2_1_15_1",
      "first-page": "3157",
      "article-title": "The biosynthesis of glycoproteins. III. Glucosamine intermediate in plasma glycoprotein synthesis in livers of puromycin\u2010treated rates",
      "volume": "239",
      "author": "Molar J.",
      "year": "1964",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_16_1",
      "unstructured": "Neuberger A. andMarshall R. D.(1966).Methods for the qualitative and quantitative analysis of the component sugars. In: Glycoproteins A. Gottschalk (Ed.) pp.190\u2013234."
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.32.1.27"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1965.tb04256.x"
    }
  ],
  "container-title": "Journal of Neurobiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fneu.480010109",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/neu.480010109",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        12
      ]
    ],
    "date-time": "2023-11-12T12:24:37Z",
    "timestamp": 1699791877000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/neu.480010109"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1969,
        1
      ]
    ]
  },
  "references-count": 17,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1969,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/neu.480010109"
  ],
  "URL": "http://dx.doi.org/10.1002/neu.480010109",
  "relation": {},
  "ISSN": [
    "0022-3034",
    "1097-4695"
  ],
  "subject": [],
  "container-title-short": "J. Neurobiol.",
  "published": {
    "date-parts": [
      [
        1969,
        1
      ]
    ]
  }
}