DOI: 10.1002/mrm.20356. Detection threshold of single SPIO‐labeled cells with FIESTA

Detection threshold of single SPIO‐labeled cells with FIESTA

10.1002/mrm.20356

Crossref journal-article

Wiley

Magnetic Resonance in Medicine (311)

Abstract

AbstractMRI of superparamagnetic iron oxide (SPIO)‐labeled cells has become a valuable tool for studying the in vivo trafficking of transplanted cells. Cellular detection with MRI is generally considered to be orders of magnitude less sensitive than other techniques, such as positron emission tomography (PET), single photon emission‐computed tomography (SPECT), or optical fluorescence microscopy. However, an analytic description of the detection threshold for single SPIO‐labeled cells and the parameters that govern detection has not been adequately provided. In the present work, the detection threshold for single SPIO‐labeled cells and the effect of resolution and SNR were studied for a balanced steady‐state free precession (SSFP) sequence (3D‐FIESTA). Based on the results from both theoretical and experimental analyses, an expression that predicts the minimum detectable mass of SPIO (mc) required to detect a single cell against a uniform signal background was derived: mc = 5v/(Kfsl · SNR), where v is the voxel volume, SNR is the image signal‐to‐noise ratio, and Kfsl is an empirical constant measured to be 6.2 ± 0.5 × 10−5 μl/pgFe. Using this expression, it was shown that the sensitivity of MRI is not very different from that of PET, requiring femtomole quantities of SPIO iron for detection under typical micro‐imaging conditions (100 μm isotropic resolution, SNR = 60). The results of this work will aid in the design of cellular imaging experiments by defining the lower limit of SPIO labeling required for single cell detection at any given resolution and SNR. Magn Reson Med 53:312–320, 2005. © 2005 Wiley‐Liss, Inc.

Bibliography

Heyn, C., Bowen, C. V., Rutt, B. K., & Foster, P. J. (2005). Detection threshold of single SPIOâlabeled cells with FIESTA. Magnetic Resonance in Medicine, 53(2), 312â320. Portico.

Authors 4

Chris Heyn (first)
Chris V. Bowen (additional)
Brian K. Rutt (additional)
Paula J. Foster (additional)

References 36 Referenced 185

10.1083/jcb.143.5.1305
10.1073/pnas.96.24.14079
10.1006/nimg.2002.1194 / Neuroimage / Tracking transplanted stem cell migration using bifunctional, contrast agent‐enhanced, magnetic resonance imaging by Modo M (2002)
10.1161/hc3601.094303
10.1097/01.TP.0000089237.39220.83
10.1038/nbt1201-1141
10.1016/S1076-6332(03)80271-4
10.1182/blood-2002-12-3669
10.1038/74464
10.1016/S0022-1759(01)00433-1
10.1002/mrm.10465
10.1148/radiology.197.2.7480707
10.1038/sj.neo.7900176
10.1073/pnas.96.26.15256
10.1148/radiol.2293021215
10.1101/gad.1047403
10.1073/pnas.242435499
{'key': 'e_1_2_8_19_2', 'first-page': '6838', 'article-title': 'In vivo high resolution three‐dimensional imaging of antigen‐specific cytotoxic T‐lymphocyte trafficking to tumors', 'volume': '63', 'author': 'Kircher MF', 'year': '2003', 'journal-title': 'Cancer Res'} / Cancer Res / In vivo high resolution three‐dimensional imaging of antigen‐specific cytotoxic T‐lymphocyte trafficking to tumors by Kircher MF (2003)
10.1016/S0006-3495(99)77182-1
10.1002/mrm.10417
10.1002/mrm.10192
10.1002/mrm.10351
10.1002/mrm.1910130207
10.1016/0022-2364(71)90047-3
10.1364/JOSA.38.000196
10.1007/s003300100908
10.1097/00126334-200401010-00002
10.1152/jappl.1977.42.3.432
10.1002/(SICI)1522-2594(199908)42:2<379::AID-MRM20>3.0.CO;2-L
10.1016/0022-1759(94)90333-6
10.1007/BF01267680
{'key': 'e_1_2_8_33_2', 'first-page': '2573', 'article-title': 'Positron emission tomography analysis of metastatic tumor cell trafficking', 'volume': '54', 'author': 'Oku N', 'year': '1994', 'journal-title': 'Cancer Res'} / Cancer Res / Positron emission tomography analysis of metastatic tumor cell trafficking by Oku N (1994)
10.1016/0005-2736(95)00123-K
{'key': 'e_1_2_8_35_2', 'first-page': '3612', 'article-title': 'Tumor cells with organ‐specific metastatic ability show distinctive trafficking in vivo: analyses by positron emission tomography and bioimaging', 'volume': '57', 'author': 'Koike C', 'year': '1997', 'journal-title': 'Cancer Res'} / Cancer Res / Tumor cells with organ‐specific metastatic ability show distinctive trafficking in vivo: analyses by positron emission tomography and bioimaging by Koike C (1997)
10.1007/BF00965836
10.1073/pnas.052709599

Dates

Type	When
Created	20 years, 7 months ago (Jan. 27, 2005, 10:48 a.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 5:50 p.m.)
Indexed	1 month ago (Aug. 2, 2025, 1:09 a.m.)
Issued	20 years, 7 months ago (Jan. 27, 2005)
Published	20 years, 7 months ago (Jan. 27, 2005)
Published Online	20 years, 7 months ago (Jan. 27, 2005)
Published Print	20 years, 7 months ago (Feb. 1, 2005)

Funders 0

None

BibTeX

@article{Heyn_2005, title={Detection threshold of single SPIO‐labeled cells with FIESTA}, volume={53}, ISSN={1522-2594}, url={http://dx.doi.org/10.1002/mrm.20356}, DOI={10.1002/mrm.20356}, number={2}, journal={Magnetic Resonance in Medicine}, publisher={Wiley}, author={Heyn, Chris and Bowen, Chris V. and Rutt, Brian K. and Foster, Paula J.}, year={2005}, month=jan, pages={312–320} }

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