DOI: 10.1002/mrm.1910290108. Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles

Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles

10.1002/mrm.1910290108

Crossref journal-article

Wiley

Magnetic Resonance in Medicine (311)

Abstract

AbstractHuman peripheral blood mononuclear cells (PBMCs) were incubated with large unilamellar vesicles (LUV) containing encapsulated dextran‐magnetite particles (DMP). This resulted in an efficient incorporation of DMP. Electron microscopy revealed the presence of DMP in cells mainly in phagosomes and secondary lysosomes. DMP‐labeled PBMCs showed a strong increase of the transverse relaxation rate (up to 16.6 s−1 for 5 x 107 cells/ml) and, accordingly, a great loss of signal intensity in MR imaging. The fraction of DMP containing PBMCs could be enriched by magnetic cell separation. The major population of the DMP containing cells proved to be monocytes. When PBMCs depleted of monocytes were used for labeling, DMP uptake was observed also in the peripheral blood lymphocytes. The labeling of PBMCs presented here may be used in future studies of selective MR imaging of in vivo cell migration in a variety of immunologically compromised tissue states, e.g., tumors, transplantations, and abscesses.

Bibliography

Bulte, J. W. M., Ma, L. D., Magin, R. L., Kamman, R. L., Hulstaert, C. E., Go, K. G., The, T. H., & De Leij, L. (1993). Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextranâmagnetite particles. Magnetic Resonance in Medicine, 29(1), 32â37. Portico.

Authors 8

Jeff W. M. Bulte (first)
Loralie D. Ma (additional)
Richard L. Magin (additional)
Richard L. Kamman (additional)
Caesar E. Hulstaert (additional)
K. Gwan Go (additional)
T. Hauw The (additional)
Lou De Leij (additional)

References 32 Referenced 113

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Dates

Type	When
Created	18 years, 6 months ago (March 5, 2007, 10:28 a.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 9:48 p.m.)
Indexed	4 months, 4 weeks ago (April 9, 2025, 6:49 a.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	19 years, 9 months ago (Nov. 18, 2005)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{Bulte_1993, title={Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles}, volume={29}, ISSN={1522-2594}, url={http://dx.doi.org/10.1002/mrm.1910290108}, DOI={10.1002/mrm.1910290108}, number={1}, journal={Magnetic Resonance in Medicine}, publisher={Wiley}, author={Bulte, Jeff W. M. and Ma, Loralie D. and Magin, Richard L. and Kamman, Richard L. and Hulstaert, Caesar E. and Go, K. Gwan and The, T. Hauw and De Leij, Lou}, year={1993}, month=jan, pages={32–37} }

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