DOI: 10.1002/mcs.1220070109. Estimation of the dissociation constant for unreacted silanol groups on the surface of ODS‐silica from the relationship between ph and electroosmotic flow velocity in capillary electrochromatography

Estimation of the dissociation constant for unreacted silanol groups on the surface of ODS‐silica from the relationship between ph and electroosmotic flow velocity in capillary electrochromatography

10.1002/mcs.1220070109

Crossref journal-article

Wiley

Journal of Microcolumn Separations (311)

Abstract

AbstractElectroosmotic flow in electrochromatography is generated by surface charges on the packing materials under an applied voltage. The surface charge density of ODS‐silica gel is increased by dissociation of unreacted silanol groups, and the dissociation constant is dependent on pH. We have developed an equation for the relationship between pH and electroosmotic flow velocity. By using this equation, we estimate the dissociation constant of unreacted silanol groups from their relationships in electrochromatography to be 10−4.3.The proposed method has the following features: (1) the dissociation constant can be estimated in situ by using a column for electrochromatography or capillary electrophoresis without any alteration of the instrumentation, and therefore (2) the dissociation constant obtained is directly useful for the selection of separation conditions.

Bibliography

Kitagawa, S., & Tsuda, T. (1995). Estimation of the dissociation constant for unreacted silanol groups on the surface of ODSâsilica from the relationship between ph and electroosmotic flow velocity in capillary electrochromatography. Journal of Microcolumn Separations, 7(1), 59â64. Portico.

Authors 2

Shinya Kitagawa (first)
Takao Tsuda (additional)

References 15 Referenced 33

10.1016/0003-2697(80)90176-1
10.1126/science.227.4694.1586
10.1016/S0021-9673(00)87275-1
10.1021/ac00168a010
10.1016/S0021-9673(01)89365-1
{'issue': '9', 'key': 'e_1_2_1_7_2', 'first-page': '26', 'volume': '5', 'author': 'Tsuda T.', 'year': '1992', 'journal-title': 'LC‐GC Intl.'} / LC‐GC Intl. by Tsuda T. (1992)
10.1016/S0021-9673(01)87127-2
10.1007/BF02321428
10.1002/mcs.1220060202
{'key': 'e_1_2_1_11_2', 'first-page': '563', 'volume-title': 'Handbook of Capillary Electrophoresis', 'author': 'Tsuda T.', 'year': '1993'} / Handbook of Capillary Electrophoresis by Tsuda T. (1993)
10.1021/ac00011a013
10.1021/ac00049a007
10.1021/ac00213a043
10.1021/ac00029a012
{'key': 'e_1_2_1_16_2', 'volume-title': 'Interfacial Phenomena', 'author': 'Davies J. T.', 'year': '1963'} / Interfacial Phenomena by Davies J. T. (1963)

Dates

Type	When
Created	20 years, 2 months ago (May 29, 2005, 8:05 a.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 8:56 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 11:01 p.m.)
Issued	30 years, 7 months ago (Jan. 1, 1995)
Published	30 years, 7 months ago (Jan. 1, 1995)
Published Online	20 years, 4 months ago (March 30, 2005)
Published Print	30 years, 7 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Kitagawa_1995, title={Estimation of the dissociation constant for unreacted silanol groups on the surface of ODS‐silica from the relationship between ph and electroosmotic flow velocity in capillary electrochromatography}, volume={7}, ISSN={1520-667X}, url={http://dx.doi.org/10.1002/mcs.1220070109}, DOI={10.1002/mcs.1220070109}, number={1}, journal={Journal of Microcolumn Separations}, publisher={Wiley}, author={Kitagawa, Shinya and Tsuda, Takao}, year={1995}, month=jan, pages={59–64} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        13
      ]
    ],
    "date-time": "2024-01-13T04:01:02Z",
    "timestamp": 1705118462134
  },
  "reference-count": 15,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            30
          ]
        ],
        "date-time": "2005-03-30T00:00:00Z",
        "timestamp": 1112140800000
      },
      "content-version": "vor",
      "delay-in-days": 3741,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Electroosmotic flow in electrochromatography is generated by surface charges on the packing materials under an applied voltage. The surface charge density of ODS\u2010silica gel is increased by dissociation of unreacted silanol groups, and the dissociation constant is dependent on pH. We have developed an equation for the relationship between pH and electroosmotic flow velocity. By using this equation, we estimate the dissociation constant of unreacted silanol groups from their relationships in electrochromatography to be 10<jats:sup>\u22124.3</jats:sup>.</jats:p><jats:p>The proposed method has the following features: (1) the dissociation constant can be estimated <jats:italic>in situ</jats:italic> by using a column for electrochromatography or capillary electrophoresis without any alteration of the instrumentation, and therefore (2) the dissociation constant obtained is directly useful for the selection of separation conditions.</jats:p>",
  "DOI": "10.1002/mcs.1220070109",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        5,
        29
      ]
    ],
    "date-time": "2005-05-29T12:05:35Z",
    "timestamp": 1117368335000
  },
  "page": "59-64",
  "source": "Crossref",
  "is-referenced-by-count": 33,
  "title": "Estimation of the dissociation constant for unreacted silanol groups on the surface of ODS\u2010silica from the relationship between ph and electroosmotic flow velocity in capillary electrochromatography",
  "prefix": "10.1002",
  "volume": "7",
  "author": [
    {
      "given": "Shinya",
      "family": "Kitagawa",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Takao",
      "family": "Tsuda",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        30
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(80)90176-1"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.227.4694.1586"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(00)87275-1"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00168a010"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(01)89365-1"
    },
    {
      "issue": "9",
      "key": "e_1_2_1_7_2",
      "first-page": "26",
      "volume": "5",
      "author": "Tsuda T.",
      "year": "1992",
      "journal-title": "LC\u2010GC Intl."
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(01)87127-2"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02321428"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/mcs.1220060202"
    },
    {
      "key": "e_1_2_1_11_2",
      "first-page": "563",
      "volume-title": "Handbook of Capillary Electrophoresis",
      "author": "Tsuda T.",
      "year": "1993"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00011a013"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00049a007"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00213a043"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac00029a012"
    },
    {
      "key": "e_1_2_1_16_2",
      "volume-title": "Interfacial Phenomena",
      "author": "Davies J. T.",
      "year": "1963"
    }
  ],
  "container-title": "Journal of Microcolumn Separations",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmcs.1220070109",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/mcs.1220070109",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        27
      ]
    ],
    "date-time": "2023-10-27T00:56:07Z",
    "timestamp": 1698368167000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/mcs.1220070109"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  },
  "references-count": 15,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1995,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/mcs.1220070109"
  ],
  "URL": "http://dx.doi.org/10.1002/mcs.1220070109",
  "relation": {},
  "ISSN": [
    "1040-7685",
    "1520-667X"
  ],
  "subject": [],
  "container-title-short": "J Microcolumn Separations",
  "published": {
    "date-parts": [
      [
        1995,
        1
      ]
    ]
  }
}