DOI: 10.1002/mc.2940040302. Expression of RB and p53 proteins in HPV‐positive and HPV‐negative cervical carcinoma cell lines

Expression of RB and p53 proteins in HPV‐positive and HPV‐negative cervical carcinoma cell lines

10.1002/mc.2940040302

Crossref journal-article

Wiley

Molecular Carcinogenesis (311)

Abstract

AbstractMutations within the tumor suppressor genes Rb‐1 and p53 are commonly found in many human malignancies, and loss of wild‐type function of both p53 and RB appear to be important events in the development of these malignancies. Interference with normal RB and p53 function in the cell has apparently also been exploited by the oncogenic genital human papillomaviruses (HPVs), which encode transforming proteins capable of binding cellular RB and p53 proteins. We have investigated the expression of RB and p53 in a series of eight cervical carcinoma cell lines, six of which contain HPV sequences and two of which have arisen apparently independently of HPV infection. In the six HPV‐positive lines, no evidence of abnormal RB or p53 protein could be detected. However, there was evidence for abnormal RB and p53 in the two HPV–negative lines. These data are consistent with the hypothesis that loss of wild‐type RB and p53 function is necessary for tumor development and that such loss can occur either by mutation within the cellular gene or by expression of viral proteins capable of complexing wild‐type cellular proteins.

Bibliography

Wrede, D., Tidy, J. A., Crook, T., Lane, D., & Vousden, K. H. (1991). Expression of RB and p53 proteins in HPVâpositive and HPVânegative cervical carcinoma cell lines. Molecular Carcinogenesis, 4(3), 171â175. Portico.

Authors 5

David Wrede (first)
John A. Tidy (additional)
Tim Crook (additional)
David Lane (additional)
Karen H. Vousden (additional)

References 32 Referenced 57

{'key': 'e_1_2_1_2_2', 'first-page': '43', 'article-title': 'Human papillomaviruses and cervical cancer', 'volume': '1', 'author': 'Vousden KH', 'year': '1989', 'journal-title': 'Cancer Cells'} / Cancer Cells / Human papillomaviruses and cervical cancer by Vousden KH (1989)
10.1073/pnas.80.12.3812
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10.1002/j.1460-2075.1989.tb08570.x / EMBO J / HPV 16 E6 and E7 proteins co‐operate to immortalize human foreskin keratinocytes by Hawley‐Nelson P (1989)
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10.1111/j.1432-1033.1986.tb09919.x
{'key': 'e_1_2_1_26_2', 'first-page': '361', 'article-title': 'Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines', 'volume': '119', 'author': 'Yee C', 'year': '1985', 'journal-title': 'Am J Pathol'} / Am J Pathol / Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines by Yee C (1985)
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CrookT WredeD TidyJ ScholefieldJ CrawfordL VousdenKHStatus of c‐myc p53 and retinoblastoma genes in human papilloma virus positive and negative squamous cell carcinomas of the anus. Oncogene (in press).

Dates

Type	When
Created	18 years, 6 months ago (Feb. 21, 2007, 3:16 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 6:13 p.m.)
Indexed	1 month, 1 week ago (July 23, 2025, 8:54 a.m.)
Issued	34 years, 8 months ago (Jan. 1, 1991)
Published	34 years, 8 months ago (Jan. 1, 1991)
Published Online	19 years, 1 month ago (July 20, 2006)
Published Print	34 years, 8 months ago (Jan. 1, 1991)

Funders 0

None

BibTeX

@article{Wrede_1991, title={Expression of RB and p53 proteins in HPV‐positive and HPV‐negative cervical carcinoma cell lines}, volume={4}, ISSN={1098-2744}, url={http://dx.doi.org/10.1002/mc.2940040302}, DOI={10.1002/mc.2940040302}, number={3}, journal={Molecular Carcinogenesis}, publisher={Wiley}, author={Wrede, David and Tidy, John A. and Crook, Tim and Lane, David and Vousden, Karen H.}, year={1991}, month=jan, pages={171–175} }

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