DOI: 10.1002/mc.2940020108. Intracellular receptor binding and nuclear transport of nerve growth factor in intact cells and a cell‐free system

Intracellular receptor binding and nuclear transport of nerve growth factor in intact cells and a cell‐free system

10.1002/mc.2940020108

Crossref journal-article

Wiley

Molecular Carcinogenesis (311)

Abstract

AbstractNuclear uptake of125I‐labeled nerve growth factor (NGF) by cells that either express or do not express the cell surface receptor was tested using intact cells and a cell‐free system. Intracellular and consequently nuclear uptake of NGF in intact cells was dependent on the presence of surface NGF receptor, whereas nuclear uptake in a cell‐free system did not correlate with cell surface receptor expression. In the cell‐free system, nuclear transport was inhibited when NGF receptor was being actively synthesized. Preincubation of intact cells with unlabeled NGF, cycloheximide, puromycin, or actinomycin D increased nuclear uptake up to threefold. The data suggest that, in intact cells, NGF transported into the cell via the surface receptors is also bound by the NGF receptor being synthesized in the cytoplasm. NGF taken up by the nucleus inhibited transcription of ribosomal RNA genes by 70% and, in turn, inhibited cell proliferation by 60%. A direct effect of NGF on transcription is discussed.

Bibliography

RakowiczâSzukzynska, E. M., Linnenbach, A. J., & Koprowski, H. (1989). Intracellular receptor binding and nuclear transport of nerve growth factor in intact cells and a cellâfree system. Molecular Carcinogenesis, 2(1), 47â58. Portico.

Authors 3

Ewa M. Rakowicz‐Szukzynska (first)
Alban J. Linnenbach (additional)
Hilary Koprowski (additional)

References 32 Referenced 17

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Dates

Type	When
Created	18 years, 6 months ago (Feb. 21, 2007, 2:32 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 12:50 p.m.)
Indexed	1 year, 10 months ago (Oct. 25, 2023, 6:46 p.m.)
Issued	36 years, 7 months ago (Jan. 1, 1989)
Published	36 years, 7 months ago (Jan. 1, 1989)
Published Online	19 years, 1 month ago (July 20, 2006)
Published Print	36 years, 7 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Rakowicz_Szukzynska_1989, title={Intracellular receptor binding and nuclear transport of nerve growth factor in intact cells and a cell‐free system}, volume={2}, ISSN={1098-2744}, url={http://dx.doi.org/10.1002/mc.2940020108}, DOI={10.1002/mc.2940020108}, number={1}, journal={Molecular Carcinogenesis}, publisher={Wiley}, author={Rakowicz‐Szukzynska, Ewa M. and Linnenbach, Alban J. and Koprowski, Hilary}, year={1989}, month=jan, pages={47–58} }

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