DOI: 10.1002/mc.20053. Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: Implications for pre‐mRNA processing

Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: Implications for pre‐mRNA processing

10.1002/mc.20053

Crossref journal-article

Wiley

Molecular Carcinogenesis (311)

Abstract

AbstractPre‐mRNA processing is an important mechanism for globally modifying cellular protein composition during tumorigenesis. To understand this process during lung cancer, expression of two key pre‐mRNA alternative splicing factors was compared in a mouse model of early lung carcinogenesis and during regenerative growth following reversible lung injury. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and alternative splicing factor/splicing factor 2 (ASF/SF2) act antagonistically to modulate splice site selection. Both hnRNP A1 and ASF/SF2 contents rose in adenomas and during injury‐induced hyperplasia compared to control lungs, as measured by immunoblotting. While both proteins increased similarly during compensatory hyperplasia, hnRNP A1 increased to a much greater extent than ASF/SF2 in tumors, resulting in a 6‐fold increase of the hnRNP A1 to ASF/SF2 ratio. Immunohistochemical analysis showed that hnRNP A1 localized exclusively within tumor nuclei, while ASF/SF2 appeared in cytoplasm and/or nuclei, depending on the growth pattern of the tumor cells. We also demonstrated cancer‐associated changes in the pre‐mRNA alternative splicing of CD44, a membrane glycoprotein involved in cell‐cell and cell‐extracellular matrix interactions. hnRNP A1 and ASF/SF2 expression is thus differentially altered in neoplastic lung cells by mechanisms that do not strictly arise from increased cell division. These changes are influenced by tumor histology and may be associated with production of variant CD44 mRNA isoforms. © 2004 Wiley‐Liss, Inc.

Bibliography

Zerbe, L. K., Pino, I., Pio, R., Cosper, P. F., DwyerâNield, L. D., Meyer, A. M., Port, J. D., Montuenga, L. M., & Malkinson, A. M. (2004). Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: Implications for preâmRNA processing. Molecular Carcinogenesis, 41(4), 187â196. Portico.

Authors 9

Laura K. Zerbe (first)
Irene Pino (additional)
Ruben Pio (additional)
Pippa F. Cosper (additional)
Lori D. Dwyer‐Nield (additional)
Amy M. Meyer (additional)
J. David Port (additional)
Luis M. Montuenga (additional)
Alvin M. Malkinson (additional)

References 40 Referenced 55

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Dates

Type	When
Created	20 years, 11 months ago (Sept. 23, 2004, 2:06 p.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 5:59 p.m.)
Indexed	1 year ago (Aug. 3, 2024, 1:53 a.m.)
Issued	20 years, 11 months ago (Sept. 23, 2004)
Published	20 years, 11 months ago (Sept. 23, 2004)
Published Online	20 years, 11 months ago (Sept. 23, 2004)
Published Print	20 years, 8 months ago (Dec. 1, 2004)

Funders 0

None

BibTeX

@article{Zerbe_2004, title={Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: Implications for pre‐mRNA processing}, volume={41}, ISSN={1098-2744}, url={http://dx.doi.org/10.1002/mc.20053}, DOI={10.1002/mc.20053}, number={4}, journal={Molecular Carcinogenesis}, publisher={Wiley}, author={Zerbe, Laura K. and Pino, Irene and Pio, Ruben and Cosper, Pippa F. and Dwyer‐Nield, Lori D. and Meyer, Amy M. and Port, J. David and Montuenga, Luis M. and Malkinson, Alvin M.}, year={2004}, month=sep, pages={187–196} }

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