DOI: 10.1002/macp.1985.021860406. Micelle‐forming block copolymers: Pinocytosis by macrophages and interaction with model membranes

Micelle‐forming block copolymers: Pinocytosis by macrophages and interaction with model membranes

10.1002/macp.1985.021860406

Crossref journal-article

Wiley

Die Makromolekulare Chemie (311)

Abstract

AbstractA block copolymer (6) with both hydrophilic and hydrophobic regions was synthesized, in order to examine its interaction with model membranes and its uptake by living cells. The copolymer comprised poly(ethylene oxide) and poly(L‐lysine) with 50 mol‐% substitution of the ε‐amino groups with palmitoyl groups. To permit 125I‐labelling, p‐methoxyphenyl residues (1–4 mol‐%) were incorporated into the block copolymer and into a poly(ethylene oxide) used for comparison. Sudan Red 7 B solubilization studies indicated that the block copolymer, but not the homopolymer, forms micelles. Differential scanning calorimetry of dipalmitoyl‐phosphatidylcholine liposomes indicated that the block copolymer interacts with and probably penetrates lipid membranes. Both poly(ethylene oxide) and the block copolymer were captured by rat peritoneal macrophages in vitro, and inhibitor studies indicated that uptake of both polymers was by pinocytosis. Rates of uptake were indicative of adsorptive pinocytosis, and it is concluded that both poly(ethylene oxide) and the block copolymer present a largely hydrophilic aspect which interacts positively with the cell surface.

Bibliography

Pratten, M. K., Lloyd, J. B., HÃ¶rpel, G., & Ringsdorf, H. (1985). Micelleâforming block copolymers: Pinocytosis by macrophages and interaction with model membranes. Die Makromolekulare Chemie, 186(4), 725â733. Portico.

Authors 4

Margaret K. Pratten (first)
John B. Lloyd (additional)
Gerhard Hörpel (additional)
Helmut Ringsdorf (additional)

References 18 Referenced 102

10.1017/CBO9780511735509.009
10.1042/bj1960049
10.1007/BF01116309
10.1016/0304-4165(82)90229-X
10.1016/0304-4165(82)90176-3
10.1042/bj1980587
{'key': 'e_1_2_1_7_2', 'volume-title': 'Titrationen in nicht wäßrigen Medien', 'author': 'Gyenes I.', 'year': '1970'} / Titrationen in nicht wäßrigen Medien by Gyenes I. (1970)
{'key': 'e_1_2_1_8_2', 'first-page': '53', 'volume-title': 'Neue Methoden in der präparativen organischen Chemie', 'author': 'Staab H. A.', 'year': '1967'} / Neue Methoden in der präparativen organischen Chemie by Staab H. A. (1967)
10.1042/bj0890114
10.1042/bj1680365
10.1042/bj1800567
10.1007/BF00255094
10.1002/bip.1976.360150922
{'key': 'e_1_2_1_14_2', 'first-page': '546', 'volume-title': 'Blockcopolymers', 'author': 'Dawkins J. V.', 'year': '1973'} / Blockcopolymers by Dawkins J. V. (1973)
10.1007/978-1-4613-4157-4_24
10.1007/BFb0048488
{'key': 'e_1_2_1_17_2', 'volume-title': 'Lipids', 'author': 'Papahadjopoulos D.', 'year': '1976'} / Lipids by Papahadjopoulos D. (1976)
10.1016/0005-2736(79)90089-0

Dates

Type	When
Created	22 years, 4 months ago (April 16, 2003, 6:25 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:17 a.m.)
Indexed	1 year ago (Sept. 1, 2024, 9:06 p.m.)
Issued	40 years, 5 months ago (April 1, 1985)
Published	40 years, 5 months ago (April 1, 1985)
Published Online	22 years, 5 months ago (March 12, 2003)
Published Print	40 years, 5 months ago (April 1, 1985)

Funders 0

None

BibTeX

@article{Pratten_1985, title={Micelle‐forming block copolymers: Pinocytosis by macrophages and interaction with model membranes}, volume={186}, ISSN={0025-116X}, url={http://dx.doi.org/10.1002/macp.1985.021860406}, DOI={10.1002/macp.1985.021860406}, number={4}, journal={Die Makromolekulare Chemie}, publisher={Wiley}, author={Pratten, Margaret K. and Lloyd, John B. and Hörpel, Gerhard and Ringsdorf, Helmut}, year={1985}, month=apr, pages={725–733} }

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