DOI: 10.1002/jss.400010308. Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents

Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents

10.1002/jss.400010308

Crossref journal-article

Wiley

Journal of Supramolecular Structure (311)

Abstract

AbstractTreatment of isolated human erythrocyte membranes with Triton X‐100 at ionic strength ⋍0.04 preferentially released all the glycerolipid and glycoprotein species. At low ionic strength, certain nonglycosylated polypeptides were also selectively solubilized. The liberated polypeptides were free of lipids, but some behaved as if associated into specific oligomeric complexes. Each detergent‐insoluble ghost residue appeared by electron microscopy to be a filamentous reticulum with adherent lipoid sheets and vesicles. The residues contained most of the membrane sphingolipids and the nonglycosylated proteins. The polypeptide elution profile obtained with nonionic detergents is therefore nearly reciprocal to that previously seen with a variety of agents which perturb proteins. These data afford further evidence that the externally‐oriented glycoproteins penetrate the membrane core where they are anchored hydrophobically, whereas the nonglycosylated polypeptides are, in general, bound by polar associations at the inner membrane surface. The filamentous meshwork of inner surface polypeptides may constitute a discrete, self‐associated continuum which provides rather than derives structural support from the membrance.

Bibliography

Yu, J., Fischman, D. A., & Steck, T. L. (1973). Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents. Journal of Supramolecular Structure, 1(3), 233â248. Portico.

Authors 3

John Yu (first)
Donald A. Fischman (additional)
Theodore L. Steck (additional)

References 56 Referenced 508

{'key': 'e_1_2_1_2_2', 'first-page': '27', 'volume-title': 'Membrane Molecular Biology', 'author': 'Steck T. L.', 'year': '1972'} / Membrane Molecular Biology by Steck T. L. (1972)
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Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 1:44 a.m.)
Deposited	1 year, 9 months ago (Nov. 1, 2023, 9:45 a.m.)
Indexed	1 day, 2 hours ago (Aug. 20, 2025, 8:48 a.m.)
Issued	52 years, 7 months ago (Jan. 1, 1973)
Published	52 years, 7 months ago (Jan. 1, 1973)
Published Online	21 years, 6 months ago (Feb. 19, 2004)
Published Print	52 years, 7 months ago (Jan. 1, 1973)

Funders 0

None

BibTeX

@article{Yu_1973, title={Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents}, volume={1}, ISSN={1547-9366}, url={http://dx.doi.org/10.1002/jss.400010308}, DOI={10.1002/jss.400010308}, number={3}, journal={Journal of Supramolecular Structure}, publisher={Wiley}, author={Yu, John and Fischman, Donald A. and Steck, Theodore L.}, year={1973}, month=jan, pages={233–248} }

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