DOI: 10.1002/jso.2930310402. Tumor measurement in the nude mouse

10.1002/jso.2930310402

Crossref journal-article

Wiley

Journal of Surgical Oncology (311)

Abstract

AbstractIn this study, the pitfalls of tumor measurement in the nude mouse were evaluated. Regarding intermethod variation, diameters of subcutaneous tumors in nude mice were expressed as length, area, and volume; tumor weights were also recorded. These measurements were all compared to a reference standard: water displacement volume. Estimates of area and volume derived from caliper measurements correlated well with water displacement volume (r = 0.97 and 0.98, respectively). At necropsy, tumor weight was the most consistent and reproducible reflection of tumor volume (r = 1.0000). Regarding interobserver variation, mean absolute difference among volumes determined by several investigators who measured the same tumors in living animals was determined. This averaged 15% of the mean calculated volume. Regarding intraobserver variation, observers measured four separate masses in nude mice eight times each. The observers were prevented from realizing that the same animals were being repeatedly evaluated. Volumes were compared in order to quantify the average variation that occurs when a single investigator repeatedly measures the same mass. When large masses were measured, this error was 7%; when small masses were measured, the error was 27%. Recommendations are made for future work employing tumor measurement.

Bibliography

Euhus, D. M., Hudd, C., Laregina, M. C., & Johnson, F. E. (1986). Tumor measurement in the nude mouse. Journal of Surgical Oncology, 31(4), 229â234. Portico.

Authors 4

David M. Euhus (first)
Charles Hudd (additional)
Marie C. Laregina (additional)
Frank E. Johnson (additional)

References 11 Referenced 495

{'key': 'e_1_2_1_2_2', 'first-page': '119', 'article-title': 'Case for using only maximum diameter in measuring tumors', 'volume': '50', 'author': 'Gurland J', 'year': '1966', 'journal-title': 'Cancer Chemother Rep'} / Cancer Chemother Rep / Case for using only maximum diameter in measuring tumors by Gurland J (1966)
{'key': 'e_1_2_1_3_2', 'first-page': '113', 'article-title': 'Some problems in evaluating tumor responses in the clinical drug evaluation program', 'volume': '50', 'author': 'Kuzma JW', 'year': '1966', 'journal-title': 'Cancer Chemother Rep'} / Cancer Chemother Rep / Some problems in evaluating tumor responses in the clinical drug evaluation program by Kuzma JW (1966)
{'key': 'e_1_2_1_4_2', 'first-page': '1787', 'article-title': 'Immunology of spontaneous mammary carcinomas in mice. V. Acquired tumor resistance and enhancement in strain A mice infected with mammary tumor virus', 'volume': '26', 'author': 'Attia MAM', 'year': '1966', 'journal-title': 'Cancer Res'} / Cancer Res / Immunology of spontaneous mammary carcinomas in mice. V. Acquired tumor resistance and enhancement in strain A mice infected with mammary tumor virus by Attia MAM (1966)
{'key': 'e_1_2_1_5_2', 'first-page': '255', 'article-title': 'Gastrin‐enhanced tumor growth of a xenotransplantable human gastric carcinoma in nude mice', 'volume': '10', 'author': 'Ohkura H', 'year': '1980', 'journal-title': 'Jpn J Clin Oncol'} / Jpn J Clin Oncol / Gastrin‐enhanced tumor growth of a xenotransplantable human gastric carcinoma in nude mice by Ohkura H (1980)
10.1038/2001294a0
10.1002/1097-0142(197607)38:1<388::AID-CNCR2820380156>3.0.CO;2-A
{'key': 'e_1_2_1_8_2', 'first-page': 'F‐11', 'volume-title': 'Handbook of Chemistry and Physics', 'author': 'Weast RC', 'year': '1976'} / Handbook of Chemistry and Physics by Weast RC (1976)
{'key': 'e_1_2_1_9_2', 'first-page': '189', 'volume-title': 'Statistics in Medicine', 'author': 'Colton T', 'year': '1974'} / Statistics in Medicine by Colton T (1974)
{'key': 'e_1_2_1_10_2', 'first-page': '107', 'article-title': 'What shall we measure on whom: Why?', 'volume': '50', 'author': 'Schneiderman MA', 'year': '1966', 'journal-title': 'Cancer Chemother Rep'} / Cancer Chemother Rep / What shall we measure on whom: Why? by Schneiderman MA (1966)
10.3109/00016348109157809
10.1097/00005072-198107000-00005

Dates

Type	When
Created	18 years, 6 months ago (Feb. 20, 2007, 5:22 a.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 12:17 p.m.)
Indexed	1 day, 8 hours ago (Sept. 4, 2025, 9:26 a.m.)
Issued	39 years, 5 months ago (April 1, 1986)
Published	39 years, 5 months ago (April 1, 1986)
Published Online	19 years, 1 month ago (July 19, 2006)
Published Print	39 years, 5 months ago (April 1, 1986)

Funders 0

None

BibTeX

@article{Euhus_1986, title={Tumor measurement in the nude mouse}, volume={31}, ISSN={1096-9098}, url={http://dx.doi.org/10.1002/jso.2930310402}, DOI={10.1002/jso.2930310402}, number={4}, journal={Journal of Surgical Oncology}, publisher={Wiley}, author={Euhus, David M. and Hudd, Charles and Laregina, Marie C. and Johnson, Frank E.}, year={1986}, month=apr, pages={229–234} }

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