DOI: 10.1002/jor.1100180207. Titanium particles inhibit osteoblast adhesion to fibronectin‐coated substrates

Titanium particles inhibit osteoblast adhesion to fibronectin‐coated substrates

10.1002/jor.1100180207

Crossref journal-article

Wiley

Journal of Orthopaedic Research (311)

Abstract

AbstractTo illuminate the effect of titanium particles on osteoblast function, we compared the adhesion force of neonatal rat calvarial osteoblasts on fibronection‐coated glass after incubation with titanium particles (80% had diameters of less than 5 μm). The cells were incubated with the particles for 1.5–72 hours. Using a micropipette single‐cell manipulation system, we showed that the adhesion force of the osteoblasts to fibronectin‐coated glass (1.0 μg/ml) was significantly affected by the presence of particulate debris. The adhesion force of the cells incubated with titanium particles for less than 4 hours was not significantly affected by exposure to the particles; after 4 hours, however, it was significantly reduced relative to that of controls. Aspiration of particle‐challenged osteoblasts into the micropipette demonstrated that the particles were not stripped from the cell surface and therefore confirmed that the osteoblasts had ingested them. During aspiration, the particles traveled through the cytoplasm rather than on the cell surface. When the osteoblasts were exposed to the particles and cytochalasin D, they exhibited much lower adhesion forces than did the controls or the cells exposed to titanium particles only; this indicates and important role of actin filaments in the osteoblastic response to particles. Staining for F‐actin also indicated an influence of internalized titanium particulate on cytoskeletal arrangement and cell spreading. Furthermore, with standard Northern blotting techniques, levels of mRNA for collagen type I and fibronectin were significantly reduced as early as 4 hours after exposure to particles compared with levels in controls, and this effect continued to 72 hours. These data indicate that direct exposure of osteoblasts to titanium particles, which we propose to be ingested by the osteoblasts, can significantly decrease osteoblast adhesion force; this may lead to decreased cellular activity and gene expression of fibronectin and collagen type I in the presence of titanium wear debris.

Bibliography

Kwon, S. Y., Takei, H., Pioletti, D. P., Lin, T., Ma, Q. J., Akeson, W. H., Wood, D. J., & Paul Sung, K. (2000). Titanium particles inhibit osteoblast adhesion to fibronectinâcoated substrates. Journal of Orthopaedic Research, 18(2), 203â211. Portico.

Authors 8

Soon Yong Kwon (first)
Hiroshi Takei (additional)
Dominique P. Pioletti (additional)
Tong Lin (additional)
Qing Jun Ma (additional)
Wayne H. Akeson (additional)
Douglas J. Wood (additional)
K‐L. Paul Sung (additional)

References 38 Referenced 25

10.2106/00004623-198870030-00005
10.2106/00004623-199072010-00021
10.1097/00003086-198304000-00005 / Clin Orthop / The biology of bone graft repair by Burchardt H (1983)
10.1016/0003-2697(87)90021-2
10.1002/jbm.820100203
10.2106/00004623-199307000-00017
10.1002/jbm.820240408
10.1016/0142-9612(96)00013-0
10.2106/00004623-199306000-00002
{'key': 'e_1_2_1_11_2', 'first-page': '56', 'article-title': 'Titanium wear debris modulates expression of c‐fos, c‐jun in mechanically stimulated osteoblasts', 'volume': '24', 'author': 'Jacobs EJ', 'year': '1998', 'journal-title': 'Trans Soc Biomater'} / Trans Soc Biomater / Titanium wear debris modulates expression of c‐fos, c‐jun in mechanically stimulated osteoblasts by Jacobs EJ (1998)
10.1097/00002281-199204000-00012
10.2106/00004623-199306000-00007
{'key': 'e_1_2_1_14_2', 'first-page': '142', 'article-title': 'A histologic and biochemical comparison of the interface tissues in cementless and cemented hip prosthesis', 'volume': '287', 'author': 'Kim KJ', 'year': '1993', 'journal-title': 'Clin Orthop'} / Clin Orthop / A histologic and biochemical comparison of the interface tissues in cementless and cemented hip prosthesis by Kim KJ (1993)
10.2106/00004623-198971090-00009
10.2106/00004623-199072070-00011
10.2106/00004623-199306000-00005
10.1097/00003086-199601000-00032
10.2106/00004623-199072040-00007
10.1097/00003086-198210000-00023 / Clin Orthop / The pathology of failed total joint arthroplasty by Mirra JM (1982)
10.1002/(SICI)1097-4636(19990905)46:3<399::AID-JBM13>3.0.CO;2-B
10.1002/jor.1100130305
10.1002/jbm.820250603
10.1002/jbm.820260303
10.1302/0301-620X.74B5.1527108 / J Bone Joint Surg [Br] / Polymethylmethacrylate‐induced inflammatory macrophages resorb bone by Quinn J (1992)
10.1002/(SICI)1097-4636(199611)32:3<355::AID-JBM8>3.0.CO;2-R
10.1002/(SICI)1097-4636(19980315)39:4<594::AID-JBM14>3.0.CO;2-7
10.1007/s004020050213
10.2106/00004623-199072020-00014 / J Bone Joint Surg [Am] / Aggressive granulomatous lesions associated with hip arthroplasty: immunopathological studies by Santavir AS (1990)
10.1002/jbm.820280111
10.1302/0301-620X.76B1.8300684
10.1126/science.3491426
10.1073/pnas.93.17.9182
10.1002/jor.1100130204
10.1002/jor.1100140508
10.1002/jbm.820110202
10.1097/00003086-199705000-00015
10.1302/0301-620X.73B4.2071635
10.2106/00004623-199701000-00011

Dates

Type	When
Created	20 years, 6 months ago (Feb. 24, 2005, 5:37 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 2:35 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 8:47 a.m.)
Issued	25 years, 6 months ago (March 1, 2000)
Published	25 years, 6 months ago (March 1, 2000)
Published Online	20 years, 6 months ago (Feb. 18, 2005)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Kwon_2000, title={Titanium particles inhibit osteoblast adhesion to fibronectin‐coated substrates}, volume={18}, ISSN={1554-527X}, url={http://dx.doi.org/10.1002/jor.1100180207}, DOI={10.1002/jor.1100180207}, number={2}, journal={Journal of Orthopaedic Research}, publisher={Wiley}, author={Kwon, Soon Yong and Takei, Hiroshi and Pioletti, Dominique P. and Lin, Tong and Ma, Qing Jun and Akeson, Wayne H. and Wood, Douglas J. and Paul Sung, K‐L.}, year={2000}, month=mar, pages={203–211} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        26
      ]
    ],
    "date-time": "2025-06-26T12:47:13Z",
    "timestamp": 1750942033539
  },
  "reference-count": 38,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            18
          ]
        ],
        "date-time": "2005-02-18T00:00:00Z",
        "timestamp": 1108684800000
      },
      "content-version": "vor",
      "delay-in-days": 1815,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        3
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>To illuminate the effect of titanium particles on osteoblast function, we compared the adhesion force of neonatal rat calvarial osteoblasts on fibronection\u2010coated glass after incubation with titanium particles (80% had diameters of less than 5 \u03bcm). The cells were incubated with the particles for 1.5\u201372 hours. Using a micropipette single\u2010cell manipulation system, we showed that the adhesion force of the osteoblasts to fibronectin\u2010coated glass (1.0 \u03bcg/ml) was significantly affected by the presence of particulate debris. The adhesion force of the cells incubated with titanium particles for less than 4 hours was not significantly affected by exposure to the particles; after 4 hours, however, it was significantly reduced relative to that of controls. Aspiration of particle\u2010challenged osteoblasts into the micropipette demonstrated that the particles were not stripped from the cell surface and therefore confirmed that the osteoblasts had ingested them. During aspiration, the particles traveled through the cytoplasm rather than on the cell surface. When the osteoblasts were exposed to the particles and cytochalasin D, they exhibited much lower adhesion forces than did the controls or the cells exposed to titanium particles only; this indicates and important role of actin filaments in the osteoblastic response to particles. Staining for F\u2010actin also indicated an influence of internalized titanium particulate on cytoskeletal arrangement and cell spreading. Furthermore, with standard Northern blotting techniques, levels of mRNA for collagen type I and fibronectin were significantly reduced as early as 4 hours after exposure to particles compared with levels in controls, and this effect continued to 72 hours. These data indicate that direct exposure of osteoblasts to titanium particles, which we propose to be ingested by the osteoblasts, can significantly decrease osteoblast adhesion force; this may lead to decreased cellular activity and gene expression of fibronectin and collagen type I in the presence of titanium wear debris.</jats:p>",
  "DOI": "10.1002/jor.1100180207",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        24
      ]
    ],
    "date-time": "2005-02-24T10:37:28Z",
    "timestamp": 1109241448000
  },
  "page": "203-211",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 25,
  "title": "Titanium particles inhibit osteoblast adhesion to fibronectin\u2010coated substrates",
  "prefix": "10.1002",
  "volume": "18",
  "author": [
    {
      "given": "Soon Yong",
      "family": "Kwon",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Hiroshi",
      "family": "Takei",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dominique P.",
      "family": "Pioletti",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tong",
      "family": "Lin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Qing Jun",
      "family": "Ma",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Wayne H.",
      "family": "Akeson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Douglas J.",
      "family": "Wood",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "K\u2010L.",
      "family": "Paul Sung",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        18
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-198870030-00005"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199072010-00021"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "crossref",
      "first-page": "28",
      "DOI": "10.1097/00003086-198304000-00005",
      "article-title": "The biology of bone graft repair",
      "volume": "174",
      "author": "Burchardt H",
      "year": "1983",
      "journal-title": "Clin Orthop"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(87)90021-2"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820100203"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199307000-00017"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820240408"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0142-9612(96)00013-0"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199306000-00002"
    },
    {
      "key": "e_1_2_1_11_2",
      "first-page": "56",
      "article-title": "Titanium wear debris modulates expression of c\u2010fos, c\u2010jun in mechanically stimulated osteoblasts",
      "volume": "24",
      "author": "Jacobs EJ",
      "year": "1998",
      "journal-title": "Trans Soc Biomater"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00002281-199204000-00012"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199306000-00007"
    },
    {
      "key": "e_1_2_1_14_2",
      "first-page": "142",
      "article-title": "A histologic and biochemical comparison of the interface tissues in cementless and cemented hip prosthesis",
      "volume": "287",
      "author": "Kim KJ",
      "year": "1993",
      "journal-title": "Clin Orthop"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-198971090-00009"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199072070-00011"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199306000-00005"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00003086-199601000-00032"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199072040-00007"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "crossref",
      "first-page": "175",
      "DOI": "10.1097/00003086-198210000-00023",
      "article-title": "The pathology of failed total joint arthroplasty",
      "volume": "170",
      "author": "Mirra JM",
      "year": "1982",
      "journal-title": "Clin Orthop"
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-4636(19990905)46:3<399::AID-JBM13>3.0.CO;2-B"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jor.1100130305"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820250603"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820260303"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "crossref",
      "first-page": "652",
      "DOI": "10.1302/0301-620X.74B5.1527108",
      "article-title": "Polymethylmethacrylate\u2010induced inflammatory macrophages resorb bone",
      "volume": "74",
      "author": "Quinn J",
      "year": "1992",
      "journal-title": "J Bone Joint Surg [Br]"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-4636(199611)32:3<355::AID-JBM8>3.0.CO;2-R"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-4636(19980315)39:4<594::AID-JBM14>3.0.CO;2-7"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004020050213"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "252",
      "DOI": "10.2106/00004623-199072020-00014",
      "article-title": "Aggressive granulomatous lesions associated with hip arthroplasty: immunopathological studies",
      "volume": "72",
      "author": "Santavir AS",
      "year": "1990",
      "journal-title": "J Bone Joint Surg [Am]"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820280111"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1302/0301-620X.76B1.8300684"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3491426"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.17.9182"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jor.1100130204"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jor.1100140508"
    },
    {
      "key": "e_1_2_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820110202"
    },
    {
      "key": "e_1_2_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00003086-199705000-00015"
    },
    {
      "key": "e_1_2_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1302/0301-620X.73B4.2071635"
    },
    {
      "key": "e_1_2_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2106/00004623-199701000-00011"
    }
  ],
  "container-title": "Journal of Orthopaedic Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjor.1100180207",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jor.1100180207",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        29
      ]
    ],
    "date-time": "2023-10-29T18:35:08Z",
    "timestamp": 1698604508000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jor.1100180207"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        3
      ]
    ]
  },
  "references-count": 38,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          2000,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jor.1100180207"
  ],
  "URL": "http://dx.doi.org/10.1002/jor.1100180207",
  "relation": {},
  "ISSN": [
    "0736-0266",
    "1554-527X"
  ],
  "subject": [],
  "container-title-short": "Journal Orthopaedic Research",
  "published": {
    "date-parts": [
      [
        2000,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "1998-06-09",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1999-07-21",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2005-02-18",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}