DOI: 10.1002/jnr.490410114. Intracellular routing of human amyloid protein precursor: Axonal delivery followed by transport to the dendrites

Intracellular routing of human amyloid protein precursor: Axonal delivery followed by transport to the dendrites

10.1002/jnr.490410114

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractA characteristic neuropathological feature of Alzheimer's disease is the cerebral deposition of amyloid plaques. These deposits contain b̃A4 amyloid peptide, a cleavage product of the transmembrane protein amyloid protein precursor (APP). Despite numerous studies on the processing of the different APP isoforms in non‐neuronal cells, little is known about its sorting and transport in neurons cf the central nervous system (CNS). To analyze this question we expressed in cultured rat hippocampal neurons the human APP 695, tagged at its N‐terminus with the myc epitope, using the Semliki forest virus (SFV) expression system. APP was first delivered from the cell body to the axon and later appeared also in the dendrites. Inhibition of protein synthesis at the time of axonal expression did not block the late appearance of the protein in the dendrites. An antibody directed against the myc tag, bound to the cell surface at 4°C at the time of axonal APP expression, could be chased to the dendritic domain after subsequent incubation at 37°C. These results suggest that the newly synthesized APP, after initial axonal delivery, may be transported to the dendrites by a transcytotic mechanism. The routing of APP in polarized neurons is different from that of polarized epithelial cells, in which the protein is delivered basolaterally, arguing for neuronal specific sorting and processing mechanisms. © 1995 Wiley‐Liss, Inc.

Bibliography

Simons, M., Ikonen, E., Tienari, P. J., CidâArregui, A., MÃ¶nning, U., Beyreuther, K., & Dotti, C. G. (1995). Intracellular routing of human amyloid protein precursor: Axonal delivery followed by transport to the dendrites. Journal of Neuroscience Research, 41(1), 121â128. Portico.

Authors 7

M. Simons (first)
E. Ikonen (additional)
P. J. Tienari (additional)
A. Cid‐Arregui (additional)
U. Mönning (additional)
K. Beyreuther (additional)
C. G. Dotti (additional)

References 34 Referenced 92

10.1016/S0021-9258(19)85394-2 / J Biol Chem / A novel zinc (II) binding site modulates the function of the bA4 amyloid protein precursor of Alzheimer's disease by Bush Al (1993)
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{'issue': '9', 'key': 'e_1_2_1_7_1', 'first-page': '1', 'article-title': "Basolateral secretion of amyloid precursor protein in Madin‐Darby canine kidney cells is disturbed by alterations of intracellular pH and by introducing a mutation associated with familial Alzheimer's disease", 'volume': '270', 'author': 'De Strooper B', 'year': '1995', 'journal-title': 'J Biol Chem'} / J Biol Chem / Basolateral secretion of amyloid precursor protein in Madin‐Darby canine kidney cells is disturbed by alterations of intracellular pH and by introducing a mutation associated with familial Alzheimer's disease by De Strooper B (1995)
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Dates

Type	When
Created	20 years, 8 months ago (Dec. 31, 2004, 9:09 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 3:17 p.m.)
Indexed	11 months, 1 week ago (Sept. 20, 2024, 11:40 a.m.)
Issued	30 years, 4 months ago (May 1, 1995)
Published	30 years, 4 months ago (May 1, 1995)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	30 years, 4 months ago (May 1, 1995)

Funders 0

None

BibTeX

@article{Simons_1995, title={Intracellular routing of human amyloid protein precursor: Axonal delivery followed by transport to the dendrites}, volume={41}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490410114}, DOI={10.1002/jnr.490410114}, number={1}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Simons, M. and Ikonen, E. and Tienari, P. J. and Cid‐Arregui, A. and Mönning, U. and Beyreuther, K. and Dotti, C. G.}, year={1995}, month=may, pages={121–128} }

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