DOI: 10.1002/jnr.490360607. Members of several gene families influence survival of rat motoneurons in vitro and in vivo

Members of several gene families influence survival of rat motoneurons in vitro and in vivo

10.1002/jnr.490360607

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractThe survival and functional maintenance of spinal motoneurons, both during the period of developmental cell death and in adulthood, have been shown to be dependent on trophic factors. In vitro experiments have previously been used to identify several survival factors for motoneurons, including CNTF, LIF, and members of the neurotrophin, FGF, and IGF gene families. Some of these factors have also been shown to be active in vivo, either on chick motoneurons during embryonic development or on lesioned facial and spinal motoneurons of the newborn rat. Here we demonstrate that lesioned newborn rat facial motoneurons can be rescued by NT‐4/5, IGF‐I and LIF. Furthermore, in contrast to chick motoneurons, the survival of isolated embryonic rat motoneurons can be maintained by the neurotrophins BDNF, NT‐3, and NT‐4/5. IGF‐I and FGF‐5 were also active in this system, each supporting more than 50% of the originally plated neurons. The responsiveness of motoneurons to multiple factors in vitro and in vivo suggests that motoneuron survival and function are regulated by the coordinated actions of members of different gene families. © 1993 Wiley‐Liss, Inc.

Bibliography

Hughes, R. A., Sendtner, M., & Thoenen, H. (1993). Members of several gene families influence survival of rat motoneurons in vitro and in vivo. Journal of Neuroscience Research, 36(6), 663â671. Portico.

Authors 3

R. A. Hughes (first)
M. Sendtner (additional)
H. Thoenen (additional)

References 72 Referenced 245

10.1126/science.2425435
10.1523/JNEUROSCI.10-11-03507.1990
10.1111/j.1471-4159.1984.tb05410.x
10.1016/0896-6273(89)90040-8
10.1016/0092-8674(92)90531-G
10.1242/dev.118.1.283 / Development / Multiple cellular signals are required for long‐term oligodendrocyte survival by Barres BA (1993)
10.1128/MCB.11.4.1840
10.1016/0896-6273(91)90287-A
10.1073/pnas.76.1.514
10.1016/0165-0270(92)90114-S
10.1083/jcb.110.4.1307
10.1016/S0021-9258(17)39810-1
10.1242/dev.119.2.545 / Development / Expression and binding characteristeic of the BDNF receptor chick trkB by Dechant G (1993)
10.1016/S0006-291X(88)80824-6
10.1016/0165-3806(86)90092-1
10.1016/0165-3806(85)90135-X
10.1111/j.1460-9568.1991.tb00031.x
10.1016/0169-328X(93)90005-A
10.1111/j.1471-4159.1975.tb11895.x
10.1146/annurev.ph.47.030185.002303
10.1073/pnas.82.24.8795
10.1083/jcb.110.3.753
10.1523/JNEUROSCI.12-08-03241.1992
10.1016/0166-2236(92)90022-Z
10.1002/aja.1001020303
10.1038/363266a0
10.1083/jcb.104.6.1623
10.1038/344339a0
10.1002/cne.901700304
10.1016/0896-6273(93)90327-N
10.1111/j.1460-9568.1993.tb00201.x
10.1016/0896-6273(93)90306-C
10.1016/0092-8674(91)90628-C
10.1016/0896-6273(92)90209-V
10.1016/0896-6273(93)90326-M
10.1016/0092-8674(91)90442-2
10.1523/JNEUROSCI.12-12-04737.1992 / J Neurosci / Coordinate expression of insulin‐like growth factor system components by neurons and neuroglia during retinal and cerebellar development by Lec WH (1992)
10.1126/science.2587985
10.1016/0165-3806(91)90074-S
10.1126/science.2321006
10.1016/0006-8993(86)91603-3
10.1016/0896-6273(92)90094-T
10.1073/pnas.86.15.5948
10.1083/jcb.119.1.45
10.1523/JNEUROSCI.06-07-02012.1986 / J Neurosci / Effects of nerve growth factor on the survival and synaptic function of la sensory neurons axotomized in neonatal rats by Miyata Y (1986)
10.1111/j.1471-4159.1991.tb02556.x / J Neurochem / Receptor binding, endocytosis, and mitogenesis of insulin‐like growth factors I and II in fetal brain neurons by Nielse FC (1991)
10.1002/ar.1092310404
10.1126/science.7682010
10.1146/annurev.ne.14.030191.002321
10.1126/science.2011743
10.1038/360755a0
10.1523/JNEUROSCI.12-07-02726.1992 / J Neurosci / The lack of effect of basic and acidic fibroblast growth factors on the naturally occurring death of neurons in the chick embryo by Oppenheim RW (1992)
10.1016/S0092-8674(05)80032-7
10.1016/0006-291X(92)90208-3
10.1038/356397a0
10.1242/dev.109.1.51 / Development / Distribution of insulin‐like growth factor peptides in the developing chick embryo by Ralphs JR (1990)
10.1002/neu.480240208
10.1016/0012-1606(90)90279-R
10.1523/JNEUROSCI.08-09-03337.1988 / J Neurosci / Binding characteristics of brain‐derived neurotrophic factor to its receptors on neurons from the chick embryo by Rodriuez‐Tébar A (1988)
10.1083/jcb.108.5.1807
10.1016/0896-6273(92)90183-E
10.1038/345440a0
10.1242/jcs.1991.Supplement_15.14
10.1038/360757a0
10.1038/358502a0
10.1016/0092-8674(91)90396-G
10.1016/0092-8674(91)90395-F
10.1038/342920a0
10.1111/j.1460-9568.1993.tb00513.x
10.1126/science.2512641
10.1038/360753a0
10.1128/MCB.8.8.3487

Dates

Type	When
Created	20 years, 7 months ago (Dec. 31, 2004, 8:46 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 12:09 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:02 a.m.)
Issued	31 years, 8 months ago (Dec. 15, 1993)
Published	31 years, 8 months ago (Dec. 15, 1993)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	31 years, 8 months ago (Dec. 15, 1993)

Funders 0

None

BibTeX

@article{Hughes_1993, title={Members of several gene families influence survival of rat motoneurons in vitro and in vivo}, volume={36}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490360607}, DOI={10.1002/jnr.490360607}, number={6}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Hughes, R. A. and Sendtner, M. and Thoenen, H.}, year={1993}, month=dec, pages={663–671} }

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