DOI: 10.1002/jnr.490310315. Hippocampal NCAM180 transiently increases sialylation during the acquisition and consolidation of a passive avoidance response in the adult rat

Hippocampal NCAM180 transiently increases sialylation during the acquisition and consolidation of a passive avoidance response in the adult rat

10.1002/jnr.490310315

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractSynaptic connectivity change is a consistent anatomical feature of memory formation and the possibility that this is mediated by a replay of neurodevelopmental events has been investigated by measuring change in neural cell adhesion molecule sialylalion state during the acquisition and consolidation of a passive avoidance response in the adult rat. The avoidance response was always generated after two to three trials and the animals remained on the platform for the criterion time of 5 min. In all cases training was complete within 5–8 min. Change in sialylation state was monitored following intrayentricuiar infusion of the 3H‐ManNAc Precursor at 4 hr prior to the reference point. No task‐specific change in general glycoconjugate sialylation was apparent in hippocampal P2 pellets at increasing times following training. Increased sialylation state was observed only in neural cell adhesion molecule (NCAM) immunoprecipitates of hippocampal membrane fractions at 12 and 24 hr after training. Change in hippocampal sialylation state could not be attributed to an increased accumulation of NCAM as detected by an immunoabsorbent assay. Immunoblotting of antibody precipitated NCAM demonstrated the 3H‐ManNAc to be incorporated into the synapse‐specific, 180 kDa isoform of NCAM and a novel 210 kDa isoform. Immunoprccipitation and immunoblotting procedures with an antibody specific for a2‐8‐polysialic acid showed the 180 and 210 kDa isoforms to be polysialylated. The role of NCAM180 sialylation as a mechanism for synapse selection in information storage is discussed.

Bibliography

Doyle, E., Nolan, P. M., Bell, R., & Regan, C. M. (1992). Hippocampal NCAM180 transiently increases sialylation during the acquisition and consolidation of a passive avoidance response in the adult rat. Journal of Neuroscience Research, 31(3), 513â523. Portico.

Authors 4

E. Doyle (first)
P. M. Nolan (additional)
R. Bell (additional)
C. M. Regan (additional)

References 51 Referenced 118

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Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 2:34 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 10:28 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:54 a.m.)
Issued	33 years, 5 months ago (March 1, 1992)
Published	33 years, 5 months ago (March 1, 1992)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	33 years, 5 months ago (March 1, 1992)

Funders 0

None

BibTeX

@article{Doyle_1992, title={Hippocampal NCAM180 transiently increases sialylation during the acquisition and consolidation of a passive avoidance response in the adult rat}, volume={31}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490310315}, DOI={10.1002/jnr.490310315}, number={3}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Doyle, E. and Nolan, P. M. and Bell, R. and Regan, C. M.}, year={1992}, month=mar, pages={513–523} }

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