DOI: 10.1002/jnr.490300213. EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture

EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture

10.1002/jnr.490300213

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractEpidermal growth factor (EGF) immunoreactive material has been demonstrated to be present in the basal ganglia. In this study, we investigated the effect of EGF on cells cultured from 16‐day embryonic rat mesencephalon, which included dopamine neurons that project to the striatum in vivo. EGF receptors were detected in untreated cultures by [125I]‐EGF binding. Treatment of the cultures with EGF resulted in up to 50‐fold increases in neuronal high‐affinity dopamine uptake. Scatchard analysis of uptake kinetics and counting of tyrosine hydroxylase‐immunore‐active cells suggest that the effect of EGF on uptake is due to increased survival and maturation of dopaminergic neurons. By contrast, the high‐affinity uptake for serotonin was increased only threefold over its controls. There was no significant effect on high‐affinity γ‐aminobutyric acid (GABA) uptake. These results suggest that EGF is acting as a neurotrophic agent preferential for dopaminergic neurons in E16 mesencephalic cultures. Immunocytochemistry for glial flbrillary acidic protein demonstrated an increase in astroglia with EGF treatment. Fluorodeoxyuridine, an agent that is toxic to proliferating cells was able to eliminate the effect of EGF on dopamine uptake, suggesting that EGF may be increasing dopaminergic cell survival largely through a population of dividing cells.

Bibliography

Casper, D., Mytilineou, C., & Blum, M. (1991). EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture. Journal of Neuroscience Research, 30(2), 372â381. Portico.

Authors 3

D. Casper (first)
C. Mytilineou (additional)
M. Blum (additional)

References 51 Referenced 138

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{'key': 'e_1_2_1_31_1', 'first-page': '1604', 'article-title': 'Epidermal growth factor receptor localization and transport within the rat brain striatonigral system', 'volume': '13', 'author': 'Leslie FM', 'year': '1987', 'journal-title': 'Soc Neurosci Abst'} / Soc Neurosci Abst / Epidermal growth factor receptor localization and transport within the rat brain striatonigral system by Leslie FM (1987)
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{'key': 'e_1_2_1_52_1', 'first-page': '999', 'article-title': 'Epidermal growth factor (EGF) enhances, in rats, dopaminergic pathway “in vivo” an Immunohistochemical study', 'volume': '16', 'author': 'Zecchinelli A', 'year': '1990', 'journal-title': 'Soc Neurosci Abst'} / Soc Neurosci Abst / Epidermal growth factor (EGF) enhances, in rats, dopaminergic pathway “in vivo” an Immunohistochemical study by Zecchinelli A (1990)

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 2:16 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 7 p.m.)
Indexed	15 hours, 4 minutes ago (Sept. 3, 2025, 6:25 a.m.)
Issued	33 years, 11 months ago (Oct. 1, 1991)
Published	33 years, 11 months ago (Oct. 1, 1991)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	33 years, 11 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{Casper_1991, title={EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture}, volume={30}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490300213}, DOI={10.1002/jnr.490300213}, number={2}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Casper, D. and Mytilineou, C. and Blum, M.}, year={1991}, month=oct, pages={372–381} }

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