DOI: 10.1002/jnr.490280204. Tyrosinated and detyrosinated microtubules in axonal processes of cerebellar macroneurons grown in culture

Tyrosinated and detyrosinated microtubules in axonal processes of cerebellar macroneurons grown in culture

10.1002/jnr.490280204

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractWe have used the monoclonal antibody YL 1/2 (Tyr) specific for tyrosinated tubulin, and a polyclonal antibody (Glu) specific for detyrosinated tubulin to visualize the distribution of microtubules and microtubule assembly sites during axonal outgrowth. Cerebellar macroneurons growing in culture initially extend several short and thin neurites which have the potential to differentiate either as axons or dendrites (Ferreira and Caceres: Developmental Brain Research 49:205‐213, 1989). At the onset of axonal outgrowth the Tyr antibody labels the minor neurites, the axon, and its growth cone, while the Glu antibody only shows immunoreactivity in the axonal shaft. After nocodazole treatment, the Tyr staining disappears, whereas that produced by the Glu antibody remains practically unchanged. When nocodazole was removed, tyrosinated microtubules reappeared first at the tip of the axon, in a more distal region than that occupied by detyrosinated microtubules; another focal site of tyrosinated tubulin incorporation was detected in the cell body. Incorporation of tyrosinated tubulin into growing axons was also studied after taxol treatment. After long incubation periods in the presence of taxol, the Tyr staining disappeared from the axon but remained in the cell body; however, immunoreactivity in this site was negative when the cells were preincubated in the presence of protein synthesis inhibitors. Release from taxol results in the reappearance of Tyr immunoreactivity at the distal end of the axon. Taken collectively, the present results indicate (1) that in cerebellar macroneurons axonal differentiation is accompanied by a temporal and spatial differentiation of microtubules fcnd (2) that there is an active site of tyrosinated tubulin assembly at the tip of axonal processes, and they suggest that the highly tyrosinated domain in this region is a consequence of rapid microtubule turnover and tubulin tyrosine ligase activity.

Bibliography

Arregui, C., Busciglio, J., Caceres, A., & Barra, H. S. (1991). Tyrosinated and detyrosinated microtubules in axonal processes of cerebellar macroneurons grown in culture. Journal of Neuroscience Research, 28(2), 171â181. Portico.

Authors 4

C. Arregui (first)
J. Busciglio (additional)
A. Caceres (additional)
H. S. Barra (additional)

References 45 Referenced 43

10.1042/bj2260311
10.1111/j.1471-4159.1978.tb12449.x
10.1111/j.1432-1033.1975.tb02435.x
10.1111/j.1471-4159.1980.tb04628.x
10.1083/jcb.103.3.917
10.1073/pnas.84.15.5272
10.1007/BF02935343
10.1016/S0021-9258(18)47779-4 / J Biol Chem / Tubulin but not microtu‐bules is the substrate for tubulin tyrosine ligase in mature avian erytrocytes by Beltramo DM (1987)
10.1523/JNEUROSCI.09-01-00358.1989 / J Neurosci / Dynamics of a‐tubulin acety‐lation in intact neurons by Black M (1989)
10.1083/jcb.105.3.1283
10.1016/0166-2236(86)90153-0
10.1038/343461a0
{'issue': '4', 'key': 'e_1_2_1_14_1', 'first-page': '82a', 'article-title': 'Kinesin heavy chain: An antibody labels membranous elements that co‐dis‐tribute with acetylated microtubules and the predicted ami‐noacid sequence of a squid heavy chain cDNA', 'volume': '109', 'author': 'Caceres A', 'year': '1989', 'journal-title': 'J Cell Biol'} / J Cell Biol / Kinesin heavy chain: An antibody labels membranous elements that co‐dis‐tribute with acetylated microtubules and the predicted ami‐noacid sequence of a squid heavy chain cDNA by Caceres A (1989)
10.1083/jcb.104.6.1569
10.1016/0968-0004(88)90103-X
10.1016/0165-3806(87)90021-6
10.1083/jcb.98.1.347
10.1002/neu.480170504
10.1016/0165-3806(87)90191-X
10.1016/0165-3806(89)90023-0
10.1016/0165-3806(89)90022-9
10.1083/jcb.100.3.764
{'key': 'e_1_2_1_24_1', 'first-page': '47', 'volume-title': 'Cell Movement, Volume 2: Kinesin, Dynein, and Microtubule Dynamics', 'author': 'Greer K', 'year': '1989'} / Cell Movement, Volume 2: Kinesin, Dynein, and Microtubule Dynamics by Greer K (1989)
10.1073/pnas.85.16.5946
10.1016/0092-8674(84)90273-3
10.1083/jcb.105.1.251
10.1083/jcb.109.5.2275
10.1083/jcb.99.4.1289
{'key': 'e_1_2_1_30_1', 'first-page': '285', 'volume-title': 'Intrinsic Determinants of Neuronal Form and Function', 'author': 'Hirokawa N', 'year': '1988'} / Intrinsic Determinants of Neuronal Form and Function by Hirokawa N (1988)
10.1083/jcb.93.3.576
10.1002/cm.970110302
10.1002/j.1460-2075.1987.tb02550.x
10.1016/S0021-9258(19)69014-9 / J Biol Chem / Preferential action of a brain detyrosino‐lating carboxypeptidase on polymeric tubulin by Kumar N (1981)
{'key': 'e_1_2_1_35_1', 'first-page': '397', 'volume-title': 'Ax‐oplasmic Transport', 'author': 'Lasek R. J.', 'year': '1981'} / Ax‐oplasmic Transport by Lasek R. J. (1981)
10.1083/jcb.109.1.253
10.1016/0896-6273(88)90124-9
10.1083/jcb.92.1.192
10.1083/jcb.107.2.651
10.1002/cm.970080207
10.1038/277665a0
10.1073/pnas.77.3.1561
10.1083/jcb.105.1.265
10.1073/pnas.84.24.9040
10.1242/jcs.88.2.185 / J Cell Sci / Turnover of the carboxi‐terminal tyrosine of α‐tubulin and means of reaching elevated levels of detyrosi‐nation in living cells by Wehland J (1987)
10.1007/BF01611985

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 12:24 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 5:38 p.m.)
Indexed	1 day, 3 hours ago (Sept. 3, 2025, 7:14 a.m.)
Issued	34 years, 7 months ago (Feb. 1, 1991)
Published	34 years, 7 months ago (Feb. 1, 1991)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	34 years, 7 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{Arregui_1991, title={Tyrosinated and detyrosinated microtubules in axonal processes of cerebellar macroneurons grown in culture}, volume={28}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490280204}, DOI={10.1002/jnr.490280204}, number={2}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Arregui, C. and Busciglio, J. and Caceres, A. and Barra, H. S.}, year={1991}, month=feb, pages={171–181} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        3
      ]
    ],
    "date-time": "2025-09-03T11:14:03Z",
    "timestamp": 1756898043444
  },
  "reference-count": 45,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            11
          ]
        ],
        "date-time": "2004-10-11T00:00:00Z",
        "timestamp": 1097452800000
      },
      "content-version": "vor",
      "delay-in-days": 5001,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        2
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>We have used the monoclonal antibody YL 1/2 (Tyr) specific for tyrosinated tubulin, and a polyclonal antibody (Glu) specific for detyrosinated tubulin to visualize the distribution of microtubules and microtubule assembly sites during axonal outgrowth. Cerebellar macroneurons growing in culture initially extend several short and thin neurites which have the potential to differentiate either as axons or dendrites (Ferreira and Caceres: <jats:italic>Developmental Brain Research</jats:italic> 49:205\u2010213, 1989). At the onset of axonal outgrowth the Tyr antibody labels the minor neurites, the axon, and its growth cone, while the Glu antibody only shows immunoreactivity in the axonal shaft. After nocodazole treatment, the Tyr staining disappears, whereas that produced by the Glu antibody remains practically unchanged. When nocodazole was removed, tyrosinated microtubules reappeared first at the tip of the axon, in a more distal region than that occupied by detyrosinated microtubules; another focal site of tyrosinated tubulin incorporation was detected in the cell body. Incorporation of tyrosinated tubulin into growing axons was also studied after taxol treatment. After long incubation periods in the presence of taxol, the Tyr staining disappeared from the axon but remained in the cell body; however, immunoreactivity in this site was negative when the cells were preincubated in the presence of protein synthesis inhibitors. Release from taxol results in the reappearance of Tyr immunoreactivity at the distal end of the axon. Taken collectively, the present results indicate (1) that in cerebellar macroneurons axonal differentiation is accompanied by a temporal and spatial differentiation of microtubules fcnd (2) that there is an active site of tyrosinated tubulin assembly at the tip of axonal processes, and they suggest that the highly tyrosinated domain in this region is a consequence of rapid microtubule turnover and tubulin tyrosine ligase activity.</jats:p>",
  "DOI": "10.1002/jnr.490280204",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        1
      ]
    ],
    "date-time": "2005-01-01T05:24:41Z",
    "timestamp": 1104557081000
  },
  "page": "171-181",
  "source": "Crossref",
  "is-referenced-by-count": 43,
  "title": "Tyrosinated and detyrosinated microtubules in axonal processes of cerebellar macroneurons grown in culture",
  "prefix": "10.1002",
  "volume": "28",
  "author": [
    {
      "given": "C.",
      "family": "Arregui",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J.",
      "family": "Busciglio",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A.",
      "family": "Caceres",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H. S.",
      "family": "Barra",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2260311"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1978.tb12449.x"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1975.tb02435.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1980.tb04628.x"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.103.3.917"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.15.5272"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02935343"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "15673",
      "DOI": "10.1016/S0021-9258(18)47779-4",
      "article-title": "Tubulin but not microtu\u2010bules is the substrate for tubulin tyrosine ligase in mature avian erytrocytes",
      "volume": "262",
      "author": "Beltramo DM",
      "year": "1987",
      "journal-title": "J Biol Chem"
    },
    {
      "issue": "1",
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "358",
      "DOI": "10.1523/JNEUROSCI.09-01-00358.1989",
      "article-title": "Dynamics of a\u2010tubulin acety\u2010lation in intact neurons",
      "volume": "9",
      "author": "Black M",
      "year": "1989",
      "journal-title": "J Neurosci"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.105.3.1283"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-2236(86)90153-0"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/343461a0"
    },
    {
      "issue": "4",
      "key": "e_1_2_1_14_1",
      "first-page": "82a",
      "article-title": "Kinesin heavy chain: An antibody labels membranous elements that co\u2010dis\u2010tribute with acetylated microtubules and the predicted ami\u2010noacid sequence of a squid heavy chain cDNA",
      "volume": "109",
      "author": "Caceres A",
      "year": "1989",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.104.6.1569"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(88)90103-X"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(87)90021-6"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.98.1.347"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/neu.480170504"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(87)90191-X"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(89)90023-0"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(89)90022-9"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.100.3.764"
    },
    {
      "key": "e_1_2_1_24_1",
      "first-page": "47",
      "volume-title": "Cell Movement, Volume 2: Kinesin, Dynein, and Microtubule Dynamics",
      "author": "Greer K",
      "year": "1989"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.16.5946"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90273-3"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.105.1.251"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.109.5.2275"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.99.4.1289"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "285",
      "volume-title": "Intrinsic Determinants of Neuronal Form and Function",
      "author": "Hirokawa N",
      "year": "1988"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.93.3.576"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cm.970110302"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1987.tb02550.x"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "crossref",
      "first-page": "7678",
      "DOI": "10.1016/S0021-9258(19)69014-9",
      "article-title": "Preferential action of a brain detyrosino\u2010lating carboxypeptidase on polymeric tubulin",
      "volume": "256",
      "author": "Kumar N",
      "year": "1981",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_1_35_1",
      "first-page": "397",
      "volume-title": "Ax\u2010oplasmic Transport",
      "author": "Lasek R. J.",
      "year": "1981"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.109.1.253"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(88)90124-9"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.92.1.192"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.107.2.651"
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cm.970080207"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/277665a0"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.3.1561"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.105.1.265"
    },
    {
      "key": "e_1_2_1_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.24.9040"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "crossref",
      "first-page": "185",
      "DOI": "10.1242/jcs.88.2.185",
      "article-title": "Turnover of the carboxi\u2010terminal tyrosine of \u03b1\u2010tubulin and means of reaching elevated levels of detyrosi\u2010nation in living cells",
      "volume": "88",
      "author": "Wehland J",
      "year": "1987",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "e_1_2_1_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01611985"
    }
  ],
  "container-title": "Journal of Neuroscience Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjnr.490280204",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jnr.490280204",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T21:38:19Z",
    "timestamp": 1698010699000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jnr.490280204"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        2
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1991,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jnr.490280204"
  ],
  "URL": "http://dx.doi.org/10.1002/jnr.490280204",
  "relation": {},
  "ISSN": [
    "0360-4012",
    "1097-4547"
  ],
  "subject": [],
  "container-title-short": "J of Neuroscience Research",
  "published": {
    "date-parts": [
      [
        1991,
        2
      ]
    ]
  }
}