DOI: 10.1002/jnr.490250404. Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro

Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro

10.1002/jnr.490250404

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractWe have developed an in vitro culture system to study the regulation of proliferation and differentiation of neural precursor cells contained within the neuroepithelium of embruonic day 10 mice. Adnumber of soluble growth factoes have been tested for their ability to regulate these early events and, of these factors, we have found that the fibroblast growth factors [FGFs] can directly stimulate the proliferation and survival of the neuroepithelial cells. At least 50% of the neuroepithekuak cekks divide in the presence of FGF whereas in the absence of FGF all of the cells die within 6 days of cultural. At higher concentrations of FGF, the cells change from being nonadherent round cells in tight clusters into a more flattened cell type which adheres to the substratum. This morphological change is accompanied by the expression of both neurofilament and GFAP, which are definitive markersof the two major cell types in the central nervous system: neurons and glia. In addition a neuroepithelial cell line, which does not rely on FGF for survival or proliferation, expresses both of these markers in response to FGF. These results indicate that FGF is stimulating the differentation of the neuroepithelial cells into mature neurons and glia.

Bibliography

Murphy, M., Drago, J., & Bartlett, P. F. (1990). Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro. Journal of Neuroscience Research, 25(4), 463â475. Portico.

Authors 3

M. Murphy (first)
J. Drago (additional)
P. F. Bartlett (additional)

References 41 Referenced 198

10.1016/0012-1606(81)90476-0
10.1038/332360a0
10.1016/0006-8993(75)90729-5
BaileyKA(1987): PhD Thesis:Identification of neural precursors in the developing brain. Melbourne. University of Melbourne.
10.1073/pnas.85.9.3255
10.1016/0304-3940(87)90260-6
10.1002/j.1460-2075.1985.tb03876.x
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10.1002/jnr.490140202
10.1073/pnas.83.19.7537
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{'key': 'e_1_2_1_42_1', 'first-page': '691', 'article-title': 'Expression of the FGF‐related oncegene int‐2 during gastrulation and neurulation in the mouse', 'volume': '7', 'author': 'Wilkinson DG', 'year': '1988', 'journal-title': 'ECBO J'} / ECBO J / Expression of the FGF‐related oncegene int‐2 during gastrulation and neurulation in the mouse by Wilkinson DG (1988)

Dates

Type	When
Created	20 years, 7 months ago (Jan. 1, 2005, 12:33 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 3:28 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:02 a.m.)
Issued	35 years, 4 months ago (April 1, 1990)
Published	35 years, 4 months ago (April 1, 1990)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	35 years, 4 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Murphy_1990, title={Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro}, volume={25}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490250404}, DOI={10.1002/jnr.490250404}, number={4}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Murphy, M. and Drago, J. and Bartlett, P. F.}, year={1990}, month=apr, pages={463–475} }

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