DOI: 10.1002/jnr.490220402. Differentiation‐regulated loss of the polysialylated embryonic form and expression of the different polypeptides of the neural cell adhesion molecule by cultured oligodendrocytes and myelin

Differentiation‐regulated loss of the polysialylated embryonic form and expression of the different polypeptides of the neural cell adhesion molecule by cultured oligodendrocytes and myelin

10.1002/jnr.490220402

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractThe expression of the neural cell adhesion molecule (N‐CAM) on cultured murine oligodendrocytes, their precursors, and myelin was examined by indirect immunofluorescence, biosynthetic radiolabeling followed by immunoprecipitation and Western blot analysis, using antibodies specific for various forms of the molecule. In all culture systems studied, whether the oligodendrocytes were cultured as an enriched fraction containing precursor cells or in the presence of astrocytes and neurons, a similar differentiation stage‐related expression of N‐CAM was seen. At early developmental stages many tetanus toxin receptor and A2B5 antigen‐positive putative oligodendrocyte precursors with bipolar morphology were seen and found to express N‐CAM in its embryonic form. Of the 04 antigen‐positive immature oligodendrocytes with few slender processes most expressed N‐CAM, but few the embryonic form of N‐CAM. The more mature 01 or 010 antigen‐positive oligodendrocytes were found to express exclusively the adult form of N‐CAM. Oligo‐dendrocytes synthesized the 120 and 140 kD forms of N‐CAM (N‐CAM 120 and N‐CAM 140), but not N‐CAM 180, although with differentiation, N‐CAM 120 predominated in oligodendrocytes and also in pure myelin. N‐CAM 120 could be released from oligodendrocytes and myelin by phosphatidylinositol‐specific phospholipase C, suggesting that in both oligodendrocytes and myelin N‐CAM 120 is inserted into the membrane by covalent linkage to phosphatidylinositol.

Bibliography

Trotter, J., BitterâSuermann, D., & Schachner, M. (1989). Differentiationâregulated loss of the polysialylated embryonic form and expression of the different polypeptides of the neural cell adhesion molecule by cultured oligodendrocytes and myelin. Journal of Neuroscience Research, 22(4), 369â383. Portico.

Authors 3

J. Trotter (first)
D. Bitter‐Suermann (additional)
M. Schachner (additional)

References 58 Referenced 121

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TrotterJ SchachnerM(1989) Cells positive for the 04 surface antigen isolated by cell sorting are able to differentiate into astrocytes or oligodendrocytes.Dev Brain Res in press.

Dates

Type	When
Created	20 years, 7 months ago (Dec. 31, 2004, 8:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 4:54 a.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:28 a.m.)
Issued	36 years, 4 months ago (April 1, 1989)
Published	36 years, 4 months ago (April 1, 1989)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	36 years, 4 months ago (April 1, 1989)

Funders 0

None

BibTeX

@article{Trotter_1989, title={Differentiation‐regulated loss of the polysialylated embryonic form and expression of the different polypeptides of the neural cell adhesion molecule by cultured oligodendrocytes and myelin}, volume={22}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490220402}, DOI={10.1002/jnr.490220402}, number={4}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Trotter, J. and Bitter‐Suermann, D. and Schachner, M.}, year={1989}, month=apr, pages={369–383} }

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