DOI: 10.1002/jnr.490170203. Translational regulation of myelin basic protein synthesis

Translational regulation of myelin basic protein synthesis

10.1002/jnr.490170203

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractSynthesis of the mouse myelin basic proteins (MBPs) was studied in reticulocyte lysates programmed with brain mRNA in the presence or absence of brain factors. Addition of brain factors to the lysates increased the incorporation of [35S] methionine into total TCA‐precipitable protein by a factor of 6–9, and the majority of this stimulation was found to be due to initiation factors. Although brain factors increased total protein synthesis, the percentage of MBP synthesis was reduced from 4.3% of the total counts incorporated (in the absence of brain factors) to 1.4% (in their presence). Increasing the concentration of brain mRNA in the lysates also reduced the relative levels of MBP synthesis. These results suggested the MBP mRNAs, as a group, were less efficiently initiated than most brain mRNAs. An analysis of the nucleotide sequence flanking the initiator codon of the MBP mRNAs indicates the presence of a second AUG codon 5 bases upstream, immediately followed by a termination codon, which may provide a structural explanation for the poorer initiation efficiency of the MBP mRNAs. Further analysis of the synthesis of the individual MBPs in the presence or absence of inhibitors of initiation (7‐methylguanosine triphosphate and aurintricarboxylic acid) and elongation (anisomycin and emetine) indicated that the 14‐kD MBP mRNA was less efficiently translated than the other MBP mRNAs. Synthesis of the 14‐kD MBP was more strongly inhibited by the initiation inhibitors than the other MBP mRNAs, and synthesis of the 14‐kD MBP was increased relative to the other MBPs in the presence of elongation inhibitors. These results are consistent with the notion that the 14‐kD MBP mRNA is initiated less efficiently than the other MBP mRNAs.

Bibliography

Campagnoni, A. T., Hunkeler, M. J., & Moskaitis, J. E. (1987). Translational regulation of myelin basic protein synthesis. Journal of Neuroscience Research, 17(2), 102â110. Portico.

Authors 3

A. T. Campagnoni (first)
M. J. Hunkeler (additional)
J. E. Moskaitis (additional)

References 34 Referenced 23

10.1073/pnas.69.6.1408
10.1016/S0021-9258(19)68469-3 / J Biol Chem / The role of mRNA competition in regulating translation. II. Comparison of the in vitro and in vivo results by Brendler T. (1981)
10.1016/0003-9861(78)90258-8
10.1111/j.1471-4159.1984.tb02744.x
10.1111/j.1471-4159.1979.tb11709.x
10.1016/0012-1606(83)90185-9
10.1016/0092-8674(85)90245-4
10.1016/S0021-9258(19)75246-6 / J Biol Chem / Importance of initiation factor preparations in the translation of reovirus and globin mRNAs lacking a 5′‐terminal 7‐methyl guanosine by Held WA (1977)
10.1016/S0021-9258(17)40513-8 / J Biol Chem / Competition between globin messenger ribonucleic acids for a discriminating initiation factor by Kabat D. (1977)
10.1016/0092-8674(78)90039-9
10.1093/nar/9.20.5233
10.1128/mr.47.1.1-45.1983 / Microbiol Rev / Comparison of initiation of protein synthesis in procaryotes, eucarytes, and organelles by Kozak M. (1983)
10.1073/pnas.83.9.2850
10.1016/S0021-9258(19)45864-X / J Biol Chem / Alpha and beta globin messenger ribonucleic acid. Different amounths and rates of initiation of translation by Lodish HF (1971)
10.1146/annurev.bi.45.070176.000351
10.1111/j.1471-4159.1980.tb07084.x
10.1146/annurev.bi.54.070185.005333
NewmanSL KitamuraK. CampagnoniAT(1987): Identification of a cDNA coding for a fifth form of myelin basic protein in mouse.Proc Natl Acad Sci USA(in press). (10.1073/pnas.84.3.886)
10.1021/bi00714a032
10.1111/j.1432-1033.1978.tb12573.x
10.1111/j.1471-4159.1981.tb00465.x
10.1016/0092-8674(83)90536-6
10.1016/S0021-9258(19)68291-8 / J Biol Chem / Translational control by messenger RNA competition for eukaryotic Initiation Factor 2 by Rosen H. (1982)
10.1111/j.1471-4159.1985.tb04025.x
10.1016/0092-8674(80)90249-4
10.1111/j.1471-4159.1986.tb12979.x
10.1016/S0092-8674(85)80109-4
10.1016/S0021-9258(19)68468-1
10.1016/S0021-9258(18)94333-4
10.1111/j.1432-1033.1981.tb05191.x
10.1111/j.1432-1033.1979.tb12845.x
10.1073/pnas.79.4.1012
10.1111/j.1471-4159.1982.tb07988.x
10.1111/j.1432-1033.1982.tb05876.x

Dates

Type	When
Created	20 years, 8 months ago (Dec. 31, 2004, 8:29 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 5:17 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 11:50 a.m.)
Issued	38 years, 8 months ago (Jan. 1, 1987)
Published	38 years, 8 months ago (Jan. 1, 1987)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	38 years, 8 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{Campagnoni_1987, title={Translational regulation of myelin basic protein synthesis}, volume={17}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490170203}, DOI={10.1002/jnr.490170203}, number={2}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Campagnoni, A. T. and Hunkeler, M. J. and Moskaitis, J. E.}, year={1987}, month=jan, pages={102–110} }

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