DOI: 10.1002/jnr.490110302. Covalent attachment of <sup>125</sup>I‐β nerve growth factor to its receptors on sympathetic neurons

Covalent attachment of 125I‐β nerve growth factor to its receptors on sympathetic neurons

10.1002/jnr.490110302

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractWhen 125I‐β nerve growth factor binds to sympathetic and sensory neurons, some labeled ligand is sequestered (becomes inaccessible to the external milieu) in a time‐ and energy‐dependent manner. It would appear that the higher affinity receptor (type I) participates in this process to a greater extent than does the lower affinity receptor (type II) [Olender and Stach, 1980; Olender et al., 1981]. A small portion of the sequestered 125I‐β nerve growth factor is found as part of a high molecular weight complex. When cells, which have been incubated with 125I‐β nerve growth factor, are solubilized with Triton X‐100 and subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, a complex with an apparent molecular weight of approximately 240,000 is obtained. The formation of the covalent complex can be prevented by the prior addition of excess unlabeled β nerve growth factor or sodium fluoride and dinitrophenol. The covalent 125I‐β nerve growth factor‐receptor complex is dissociated in 50 mM dithiothreitol indicating that disulfide linkages are involved. At concentrations of β nerve growth factor (3.8 × 10−11 −3.8 × 10−10 M) where maximal fiber outgrowth occurs in vitro, approximately 50‐266 attomoles (0.3‐1.6% of the type I receptors) of the covalent complex are formed per 107 nerve cells. These data suggest that a small portion of the 125I‐β nerve growth sequestered by sympathetic neurons becomes covalently attached to its receptor subsequent to its sequestration in a manner which appears to involve type I receptors.

Bibliography

Stach, R. W., & Rusenko, K. W. (1984). Covalent attachment of 125IâÎ² nerve growth factor to its receptors on sympathetic neurons. Journal of Neuroscience Research, 11(3), 215â229. Portico.

Authors 2

R. W. Stach (first)
K. W. Rusenko (additional)

References 40 Referenced 10

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10.1016/S0021-9258(17)44243-8 / J Biol Chem / Disulfide exchange between insulin and its receptor by Clark S (1983)
10.1016/S0021-9258(19)68322-5 / J Biol Chem / Direct linkage of 125I‐EGF to cell surface receptors by Comens PG (1982)
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10.1016/0006-291X(80)91279-6
{'key': 'e_1_2_1_15_1', 'first-page': '223', 'article-title': 'Detection of two types of nerve growth factor receptors by affinity labeling', 'volume': '14', 'author': 'Hosang M', 'year': '1983', 'journal-title': 'Trans Am Soc Neurochem'} / Trans Am Soc Neurochem / Detection of two types of nerve growth factor receptors by affinity labeling by Hosang M (1983)
10.1016/S0021-9258(17)34631-8 / J Biol Chem / The fate of insulin bound to adipocytes by Kahn CR (1978)
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10.1016/S0021-9258(19)70567-5 / J Biol Chem / Sequestration of 125I‐labeled β nerve growth factor by sympathetic neurons by Olender EJ (1980)
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{'key': 'e_1_2_1_29_1', 'volume-title': "Master's Thesis submitted to SUNY Upstate Medical Center", 'author': 'Rusenko KW', 'year': '1982'} / Master's Thesis submitted to SUNY Upstate Medical Center by Rusenko KW (1982)
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Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 12:29 a.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 5:55 p.m.)
Indexed	1 year, 10 months ago (Oct. 21, 2023, 4:05 p.m.)
Issued	41 years, 8 months ago (Jan. 1, 1984)
Published	41 years, 8 months ago (Jan. 1, 1984)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	41 years, 8 months ago (Jan. 1, 1984)

Funders 0

None

BibTeX

@article{Stach_1984, title={Covalent attachment of 125I‐β nerve growth factor to its receptors on sympathetic neurons}, volume={11}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490110302}, DOI={10.1002/jnr.490110302}, number={3}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Stach, R. W. and Rusenko, K. W.}, year={1984}, month=jan, pages={215–229} }

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