DOI: 10.1002/jnr.490110204. Formation of 10‐nanometer filaments from the 150k‐dalton neurofilament protein in vitro

Formation of 10‐nanometer filaments from the 150k‐dalton neurofilament protein in vitro

10.1002/jnr.490110204

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractIn the present study we report self‐assembly of individual neurofilament (NF) triplet proteins (70K, 150K, and 200K daltons) isolated by anion exchange chromatography from bovine spinal cord. Formation of smooth 10‐nm filaments by both NF 150K and NF 70K is shown. Optimal conditions for NK 150K filament formation were incubation in 100 mM MES, 0.2 M NaCl, 1 mM DTT, 0.5 mM EGTA, pH 6.5, at 37°C for 24 hr. Under the same assembly conditions, NF 200K formed 7‐nm coiled structures. These thin filaments were similar to those formed by NF 70K and 150K under less than optimal conditions. Our results indicate that NF 150K is an integral part of the filament (self‐assembly of NF 70K was previously demonstrated by others). We suggest that the optimal conditions resulting in the formation of a 10‐nm 200K homopolymer remain to be determined and that the thin coiled structures formed by all three NF proteins are protofilaments that coalesce to form a double helical 10‐nm filament.

Bibliography

Gardner, E. E., Dahl, D., & Bignami, A. (1984). Formation of 10ânanometer filaments from the 150kâdalton neurofilament protein in vitro. Journal of Neuroscience Research, 11(2), 145â155. Portico.

Authors 3

E. E. Gardner (first)
D. Dahl (additional)
A. Bignami (additional)

References 25 Referenced 50

10.1016/0014-4827(83)90352-X
10.1016/0014-4886(81)90100-X
10.1016/0003-2697(82)90123-3
10.1002/j.1460-2075.1983.tb01584.x
10.1016/0022-2836(81)90011-5
10.1083/jcb.66.2.351
10.1038/227680a0
10.1021/bi00256a029
10.1083/jcb.79.3.637
10.1016/S0021-9258(19)52451-6
10.1007/BF00233488
10.1083/jcb.94.3.592
10.1083/jcb.89.3.560
10.1016/0003-2697(80)90470-4
10.1016/0022-2836(81)90303-X
10.1016/0022-2836(79)90340-1
10.1083/jcb.78.3.653
10.1083/jcb.78.2.369
10.1242/jcs.23.1.243 / J Cell Sci / Studies on the function and composition of the 10‐nm (100‐Å) filaments of vertebrate smooth muscle by Small JV (1977)
10.1523/JNEUROSCI.01-05-00461.1981 / J Neurosci / The loss of neuronspecific proteins during the course of Wallerian degeneration of optic and sciatic nerve by Soifer D (1981)
10.1016/0022-2836(78)90376-5
10.1016/S0021-9258(20)82068-7 / J Biol Chem / The distribution of mass in heteropolymer intermediate filaments assembled in vitro. STEM analysis of vimentin/desmin and bovine epidermal keratin by Steven AC (1983)
10.1016/S0021-9258(18)94333-4
10.1083/jcb.89.2.198
10.1073/pnas.79.3.754

Dates

Type	When
Created	20 years, 7 months ago (Jan. 1, 2005, 5:24 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 4:46 a.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	41 years, 7 months ago (Jan. 1, 1984)
Published	41 years, 7 months ago (Jan. 1, 1984)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	41 years, 7 months ago (Jan. 1, 1984)

Funders 0

None

BibTeX

@article{Gardner_1984, title={Formation of 10‐nanometer filaments from the 150k‐dalton neurofilament protein in vitro}, volume={11}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490110204}, DOI={10.1002/jnr.490110204}, number={2}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Gardner, E. E. and Dahl, D. and Bignami, A.}, year={1984}, month=jan, pages={145–155} }

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