DOI: 10.1002/jnr.490080233. Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system

Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system

10.1002/jnr.490080233

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractBinding of 125I‐epidermal growth factor (EGF) to purified populations of rat astrocytes, oligodendrocytes, and neurons was measured. Astrocytes bound 40,000–100,000 EGF molecules per cell, while oligodendrocytes bound only 6,000–10,000 EGF molecules per cell. In contrast, neurons had little or no capacity to bind 125I‐EGF. EGF alone was able to stimulate incorporation of tritiated thymidine fivefold in purified astrocyte cultures incubated in serum‐free medium. When EGF was added to the previously described chemically defined medium for astrocytes, incorporation of tritiated thymidine in purified astrocytes was equivalent to that observed in medium containing 10% fetal calf serum. Addition of EGF to this chemically defined medium also doubled the proliferative capability of the medium for cultured astrocytes. EGF was maximally effective in stimulating 3H‐thymidine incorporation at concentrations between 1–10 ng/ml.

Bibliography

Simpson, D. L., Morrison, R., de Vellis, J., & Herschman, H. R. (1982). Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system. Journal of Neuroscience Research, 8(2â3), 453â462. Portico.

Authors 4

D. L. Simpson (first)
R. Morrison (additional)
J. de Vellis (additional)
H. R. Herschman (additional)

References 25 Referenced 176

10.1016/S0021-9258(17)34785-3 / J Biol Chem / Epidermal growth factor: Relationship between receptor regulation and mitogenesis in 3T3 cells by Aharonov A (1978)
10.1146/annurev.bi.48.070179.001205
10.1016/0092-8674(80)90366-9
10.1002/jcp.1041050211
10.1083/jcb.82.3.820
{'key': 'e_1_2_1_7_1', 'volume-title': 'Nervous System Regeneration', 'author': 'DeVellis J', 'year': '1982'} / Nervous System Regeneration by DeVellis J (1982)
10.1126/science.176.4034.482
10.1038/287553a0
10.1083/jcb.84.3.495
10.1146/annurev.ph.43.030181.001343
10.1146/annurev.ne.03.030180.002033
10.1016/0092-8674(80)90367-0
10.1016/0006-291X(79)90960-4
10.1016/0165-3806(81)90118-8
10.1007/BF00210517
10.1016/S0079-6123(08)60993-0
{'key': 'e_1_2_1_18_1', 'first-page': '619', 'article-title': 'Immunosympathectomy', 'volume': '18', 'author': 'Levi‐Montalcini R', 'year': '1966', 'journal-title': 'Pharmacol Rev'} / Pharmacol Rev / Immunosympathectomy by Levi‐Montalcini R (1966)
10.1073/pnas.46.3.384 / Proc Natl Acad Sci USA / Destruction of sympathetic ganglia in mammals by an antiserum to nerve growth factor by Levi‐Montalcini R (1960)
10.1002/jnr.490050510
10.1083/jcb.85.3.890
10.1073/pnas.78.11.7205
{'key': 'e_1_2_1_23_1', 'first-page': '7608', 'article-title': 'Epidermal growth factor and a new derivative', 'volume': '247', 'author': 'Savage CR', 'year': '1972', 'journal-title': 'J Biol Chem'} / J Biol Chem / Epidermal growth factor and a new derivative by Savage CR (1972)
10.1073/pnas.78.12.7535
10.1016/0092-8674(82)90163-5
10.1016/S0021-9258(19)70489-X / J Biol Chem / Increased phosphorylation of specific nuclear proteins in superior cervical ganglia and PC‐12 cells in response to nerve growth factor by Yu MW (1980)

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 5:42 a.m.)
Deposited	2 years ago (Aug. 31, 2023, 8:11 a.m.)
Indexed	2 months, 3 weeks ago (June 12, 2025, 5:43 a.m.)
Issued	43 years, 8 months ago (Jan. 1, 1982)
Published	43 years, 8 months ago (Jan. 1, 1982)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	43 years, 8 months ago (Jan. 1, 1982)

Funders 0

None

BibTeX

@article{Simpson_1982, title={Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system}, volume={8}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490080233}, DOI={10.1002/jnr.490080233}, number={2–3}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Simpson, D. L. and Morrison, R. and de Vellis, J. and Herschman, H. R.}, year={1982}, month=jan, pages={453–462} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        12
      ]
    ],
    "date-time": "2025-06-12T09:43:49Z",
    "timestamp": 1749721429999
  },
  "reference-count": 25,
  "publisher": "Wiley",
  "issue": "2-3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            11
          ]
        ],
        "date-time": "2004-10-11T00:00:00Z",
        "timestamp": 1097452800000
      },
      "content-version": "vor",
      "delay-in-days": 8319,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1982,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Binding of <jats:sup>125</jats:sup>I\u2010epidermal growth factor (EGF) to purified populations of rat astrocytes, oligodendrocytes, and neurons was measured. Astrocytes bound 40,000\u2013100,000 EGF molecules per cell, while oligodendrocytes bound only 6,000\u201310,000 EGF molecules per cell. In contrast, neurons had little or no capacity to bind <jats:sup>125</jats:sup>I\u2010EGF. EGF alone was able to stimulate incorporation of tritiated thymidine fivefold in purified astrocyte cultures incubated in serum\u2010free medium. When EGF was added to the previously described chemically defined medium for astrocytes, incorporation of tritiated thymidine in purified astrocytes was equivalent to that observed in medium containing 10% fetal calf serum. Addition of EGF to this chemically defined medium also doubled the proliferative capability of the medium for cultured astrocytes. EGF was maximally effective in stimulating <jats:sup>3</jats:sup>H\u2010thymidine incorporation at concentrations between 1\u201310 ng/ml.</jats:p>",
  "DOI": "10.1002/jnr.490080233",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        1
      ]
    ],
    "date-time": "2005-01-01T10:42:15Z",
    "timestamp": 1104576135000
  },
  "page": "453-462",
  "source": "Crossref",
  "is-referenced-by-count": 176,
  "title": "Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system",
  "prefix": "10.1002",
  "volume": "8",
  "author": [
    {
      "given": "D. L.",
      "family": "Simpson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "R.",
      "family": "Morrison",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J.",
      "family": "de Vellis",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H. R.",
      "family": "Herschman",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "3970",
      "DOI": "10.1016/S0021-9258(17)34785-3",
      "article-title": "Epidermal growth factor: Relationship between receptor regulation and mitogenesis in 3T3 cells",
      "volume": "253",
      "author": "Aharonov A",
      "year": "1978",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.48.070179.001205"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(80)90366-9"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041050211"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.82.3.820"
    },
    {
      "key": "e_1_2_1_7_1",
      "volume-title": "Nervous System Regeneration",
      "author": "DeVellis J",
      "year": "1982"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.176.4034.482"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/287553a0"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.84.3.495"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ph.43.030181.001343"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ne.03.030180.002033"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(80)90367-0"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(79)90960-4"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(81)90118-8"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00210517"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6123(08)60993-0"
    },
    {
      "key": "e_1_2_1_18_1",
      "first-page": "619",
      "article-title": "Immunosympathectomy",
      "volume": "18",
      "author": "Levi\u2010Montalcini R",
      "year": "1966",
      "journal-title": "Pharmacol Rev"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "crossref",
      "first-page": "384",
      "DOI": "10.1073/pnas.46.3.384",
      "article-title": "Destruction of sympathetic ganglia in mammals by an antiserum to nerve growth factor",
      "volume": "46",
      "author": "Levi\u2010Montalcini R",
      "year": "1960",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jnr.490050510"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.85.3.890"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.11.7205"
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "7608",
      "article-title": "Epidermal growth factor and a new derivative",
      "volume": "247",
      "author": "Savage CR",
      "year": "1972",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.12.7535"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(82)90163-5"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "crossref",
      "first-page": "10481",
      "DOI": "10.1016/S0021-9258(19)70489-X",
      "article-title": "Increased phosphorylation of specific nuclear proteins in superior cervical ganglia and PC\u201012 cells in response to nerve growth factor",
      "volume": "255",
      "author": "Yu MW",
      "year": "1980",
      "journal-title": "J Biol Chem"
    }
  ],
  "container-title": "Journal of Neuroscience Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjnr.490080233",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jnr.490080233",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        8,
        31
      ]
    ],
    "date-time": "2023-08-31T12:11:16Z",
    "timestamp": 1693483876000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jnr.490080233"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1982,
        1
      ]
    ]
  },
  "references-count": 25,
  "journal-issue": {
    "issue": "2-3",
    "published-print": {
      "date-parts": [
        [
          1982,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jnr.490080233"
  ],
  "URL": "http://dx.doi.org/10.1002/jnr.490080233",
  "relation": {},
  "ISSN": [
    "0360-4012",
    "1097-4547"
  ],
  "subject": [],
  "container-title-short": "J of Neuroscience Research",
  "published": {
    "date-parts": [
      [
        1982,
        1
      ]
    ]
  }
}