DOI: 10.1002/jnr.490060304. Synthesis of lipids in mouse brain cell cultures during development

Synthesis of lipids in mouse brain cell cultures during development

10.1002/jnr.490060304

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractSeveral metabolic activities in dissociated cultures of newborn mouse brain were compared to the situation in vivo. The developmental activity pattern of cerebroside‐sulfotransferase, cyclic nucleotide phosphohydrolase, and β‐hydroxy‐β‐methyl glutaryl‐coenzyme A‐reductase and the synthesis and deposition of sulfatide and cholesterol in culture were estimated. The enzyme activity patterns in vivo and in culture are the same. Since the cultures show very little myelin formation, the parallel increase of enzyme activities necessary for myelination in vivo and in culture suggest the existence of intrinsic factors regulating the biochemical differentiation. In addition, the formation of the products, determined in culture, follows the patterns of the enzyme activities. Dissociated brain cell cultures are therefore a valid model for the study of biochemical parameters related to the synthesis of brain lipids during development.

Bibliography

Siegrist, H. P., BologaâSandru, L., Burkart, T., Wiesmann, U., Hofmann, K., & Herschkowitz, N. (1981). Synthesis of lipids in mouse brain cell cultures during development. Journal of Neuroscience Research, 6(3), 293â301. Portico.

Authors 6

Hans Peter Siegrist (first)
Liane Bologa‐Sandru (additional)
Thomas Burkart (additional)
Ulrich Wiesmann (additional)
Karel Hofmann (additional)
Norbert Herschkowitz (additional)

References 21 Referenced 27

10.1016/0006-8993(72)90144-8
10.1016/0006-8993(81)90895-7
10.1016/0005-2744(77)90058-4
BurkartT HofmannK SiegristHP HerschkowitzNN WiesmannUN(1981): Quantitative measurement of the in vivo sulfatide metabolism during development of the mouse brain: Evidence for a large rapidly degradable sulfatide pool.Dev Biol(in press). (10.1016/S0012-1606(81)80006-1)
10.1016/S0021-9258(18)61991-X / J Biol Chem / Characterisation of cerebroside‐sulfotransferase from rat brain by Farrell DF (1971)
10.1016/S0021-9258(18)64849-5
10.1016/0003-2697(71)90476-3
10.1016/S0022-2275(20)36813-9 / J Lipid Res / Synthesis and turnover of cerebroside‐sulfate of myelin in adult and developing rat brain by Jungalwala FB (1974)
10.1016/S0022-2275(20)43098-6 / J Lipid Res / Rapid, sensitive spectrophotometric methods for quantitative determination of sulfatides by Kean EL (1968)
10.1016/0024-3205(71)90303-1
10.1016/S0021-9258(19)52451-6
10.1016/0006-8993(76)90691-0
10.1016/0006-8993(76)90664-8
{'key': 'e_1_2_1_15_1', 'first-page': '70', 'article-title': 'Some properties of PAPS‐cerebroside‐cerebroside‐sulfotransferase in jimpy and quaking mice', 'volume': '2', 'author': 'Sarliève LL', 'year': '1972', 'journal-title': 'Neurobiology'} / Neurobiology / Some properties of PAPS‐cerebroside‐cerebroside‐sulfotransferase in jimpy and quaking mice by Sarliève LL (1972)
10.1016/0006-8993(80)90008-6
10.1111/j.1471-4159.1976.tb12288.x
10.1016/0005-2760(79)90211-X
10.1203/00006450-198011000-00015
10.1111/j.1471-4159.1978.tb10779.x
{'key': 'e_1_2_1_21_1', 'first-page': '305', 'article-title': 'Dissociated cultures of newborn mouse brain. I. Metabolism of sulfated lipids and mucopolysaccharides', 'volume': '5', 'author': 'Wiesmann UN', 'year': '1975', 'journal-title': 'Neurobiology'} / Neurobiology / Dissociated cultures of newborn mouse brain. I. Metabolism of sulfated lipids and mucopolysaccharides by Wiesmann UN (1975)
{'key': 'e_1_2_1_22_1', 'first-page': '486', 'article-title': 'A new method for cholesterol determination', 'volume': '41', 'author': 'Zlatkis A', 'year': '1973', 'journal-title': 'J Lab Clin Med'} / J Lab Clin Med / A new method for cholesterol determination by Zlatkis A (1973)

Dates

Type	When
Created	20 years, 7 months ago (Jan. 1, 2005, 7:50 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 10:46 p.m.)
Indexed	1 year, 9 months ago (Nov. 11, 2023, 11:10 p.m.)
Issued	44 years, 7 months ago (Jan. 1, 1981)
Published	44 years, 7 months ago (Jan. 1, 1981)
Published Online	20 years, 10 months ago (Oct. 11, 2004)
Published Print	44 years, 7 months ago (Jan. 1, 1981)

Funders 0

None

BibTeX

@article{Siegrist_1981, title={Synthesis of lipids in mouse brain cell cultures during development}, volume={6}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.490060304}, DOI={10.1002/jnr.490060304}, number={3}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Siegrist, Hans Peter and Bologa‐Sandru, Liane and Burkart, Thomas and Wiesmann, Ulrich and Hofmann, Karel and Herschkowitz, Norbert}, year={1981}, month=jan, pages={293–301} }

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