DOI: 10.1002/jnr.20041. Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord

Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord

10.1002/jnr.20041

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractNuclear magnetic resonance (MR) imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied, in animals with a cortical photochemical lesion or with a balloon‐induced spinal cord compression lesion, the fate of implanted rat bone marrow stromal cells (MSCs) and mouse embryonic stem cells (ESCs) labeled with superparamagnetic iron oxide nanoparticles (Endorem). MSCs were colabeled with bromodeoxyuridine (BrdU), and ESCs were transfected with pEGFP‐C1 (eGFP ESCs). Cells were either grafted intracerebrally into the contralateral hemisphere of the adult rat brain or injected intravenously. In vivo MR imaging was used to track their fate; Prussian blue staining and electron microscopy confirmed the presence of iron oxide nanoparticles inside the cells. During the first week postimplantation, grafted cells migrated to the lesion site and populated the border zone of the lesion. Less than 3% of MSCs differentiated into neurons and none into astrocytes; 5% of eGFP ESCs differentiated into neurons, whereas 70% of eGFP ESCs became astrocytes. The implanted cells were visible on MR images as a hypointense area at the injection site, in the corpus callosum and in the lesion. The hypointense signal persisted for more than 50 days. The presence of GFP‐positive or BrdU‐positive and nanoparticle‐labeled cells was confirmed by histological staining. Our study demonstrates that both grafted MSCs and eGFP ESCs labeled with a contrast agent based on iron oxide nanoparticles migrate into the injured CNS. Iron oxide nanoparticles can therefore be used as a marker for the long‐term noninvasive MR tracking of implanted stem cells. © 2004 Wiley‐Liss, Inc.

Bibliography

JendelovÃ¡, P., Herynek, V., UrdzÃkovÃ¡, L., GlogarovÃ¡, K., KroupovÃ¡, J., Andersson, B., Bryja, V., Burian, M., HÃ¡jek, M., & SykovÃ¡, E. (2004). Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. Journal of Neuroscience Research, 76(2), 232â243. Portico.

Authors 10

Pavla Jendelová (first)
Vít Herynek (additional)
Lucia Urdzíková (additional)
Kateřina Glogarová (additional)
Jana Kroupová (additional)
Benita Andersson (additional)
Vítězslav Bryja (additional)
Martin Burian (additional)
Milan Hájek (additional)
Eva Syková (additional)

References 40 Referenced 245

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Dates

Type	When
Created	21 years, 5 months ago (March 24, 2004, 3:04 a.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 3 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:53 a.m.)
Issued	21 years, 5 months ago (March 8, 2004)
Published	21 years, 5 months ago (March 8, 2004)
Published Online	21 years, 5 months ago (March 8, 2004)
Published Print	21 years, 4 months ago (April 15, 2004)

Funders 3

Ministry of Education, Sports and Youth of the Czech Republic
Awards2
1. J13/98: 11120004
2. LN00A065
Academy of Sciences of the Czech Republic 10.13039/501100004240 Akademie Věd České Republiky
Region: Europe
gov (National government)
Labels9
1. Akademie věd ČR
2. Academy of Sciences of the Czechia
3. CzechAcademy
4. Czechoslovak Academy of Sciences
5. Academy of Sciences of the Czech Republic
6. Akademie věd ČR
7. Czech Academy of Sciences
8. AV ČR
9. CAS
Awards1
1. AVOZ5039906-1024
Grant Agency of the Czech Republic 10.13039/501100001824 Grantová Agentura České Republiky
Region: Europe
pri (Trusts, charities, foundations (both public and private))
Labels9
1. Grant Agency of the Czech Republic
2. Czech Science Foundation
3. Grantová agentura ČR
4. Grantové agentury
5. GrantovaAgentura
6. GAČR
7. GACR
8. GA ČR
9. GA CR
Awards1
1. 304/03/1189

BibTeX

@article{Jendelov__2004, title={Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord}, volume={76}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.20041}, DOI={10.1002/jnr.20041}, number={2}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Jendelová, Pavla and Herynek, Vít and Urdzíková, Lucia and Glogarová, Kateřina and Kroupová, Jana and Andersson, Benita and Bryja, Vítězslav and Burian, Martin and Hájek, Milan and Syková, Eva}, year={2004}, month=mar, pages={232–243} }

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