DOI: 10.1002/jnr.1123. Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells

Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells

10.1002/jnr.1123

Crossref journal-article

Wiley

Journal of Neuroscience Research (311)

Abstract

AbstractThe aim of our study was to investigate whether a human neural cell line could be used as a reliable screening tool to examine the functional conservation, in humans, of transcription factors involved in neuronal or glial specification in other species. Gain‐of‐function experiments were performed on DEV cells, a cell line derived from a human medulloblastoma. Genes encoding nine different transcription factors were tested for their influence on the process of specification of human DEV cells towards a neuronal or glial fate. In a first series of experiments, DEV cells were transfected with murine genes encoding transcription factors known to be involved in the neuronal differentiation cascade. Neurogenins‐1, ‐2, and ‐3; Mash‐1; and NeuroD increased the differentiation of DEV cells towards a neuronal phenotype by a factor of 2–3.5. In a second series of experiments, we tested transcription factors involved in invertebrate glial specification. In the embryonic Drosophila CNS, the development of most glial cells depends on the master regulatory gene glial cell missing (gcm). Expression of gcm in DEV cells induced a twofold increase of astrocytic and a sixfold increase of oligodendroglial cell types. Interestingly, expression of tramtrack69, which is required in all Drosophila glial cells, resulted in a fourfold increase of only the oligodendrocyte phenotype. Expression of the related tramtrack88 protein, which is not expressed in the fly glia, or the C. elegans lin26 protein showed no effect. These results show that the Drosophila transcription factor genes tested can conserve their function upon transfection into the human DEV cells, qualifying this cell line as a screening tool to analyze the mechanisms of neuronal and glial specification. J. Neurosci. Res. 65:17–23, 2001. © 2001 Wiley‐Liss, Inc.

Bibliography

Buzanska, L., Spassky, N., Belin, M. F., Giangrande, A., Guillemot, F., KlÃ¤mbt, C., Labouesse, M., Thomas, J. L., DomanskaâJanik, K., & Zalc, B. (2001). Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells. Journal of Neuroscience Research, 65(1), 17â23. Portico.

Authors 10

L. Buzanska (first)
N. Spassky (additional)
M.F. Belin (additional)
A. Giangrande (additional)
F. Guillemot (additional)
C. Klämbt (additional)
M. Labouesse (additional)
J.L. Thomas (additional)
K. Domanska‐Janik (additional)
B. Zalc (additional)

References 40 Referenced 13

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Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 5:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 18, 2023, 2:50 p.m.)
Indexed	6 months, 1 week ago (Feb. 20, 2025, 9:04 p.m.)
Issued	24 years, 2 months ago (June 26, 2001)
Published	24 years, 2 months ago (June 26, 2001)
Published Online	24 years, 2 months ago (June 26, 2001)
Published Print	24 years, 1 month ago (July 1, 2001)

Funders 4

INSERM 10.13039/501100001677 Institut National de la Santé et de la Recherche Médicale
Region: Europe
gov (Research institutes and centers)
Labels4
1. French National Institute for Health and Medical Research
2. French National Institute of Health & Medical Research
3. National Institute of Health and Medical Research
4. Inserm
European Leucodystrophy Association
Association de Recherche sur la Sclérose En Plaques
European Commission 10.13039/501100000780
Region: Europe
gov (National government)
Labels26
1. European Union
2. Comisión Europea
3. Europäische Kommission
4. EU-Kommissionen
5. Euroopa Komisjoni
6. Ευρωπαϊκής Επιτροπής
7. Европейската комисия
8. Evropské komise
9. Commission européenne
10. Choimisiúin Eorpaigh
11. Europskoj komisiji
12. Commissione europea
13. La Commissione europea
14. Eiropas Komisiju
15. Europos Komisijos
16. Európai Bizottságról
17. Europese Commissie
18. Komisja Europejska
19. Comissão Europeia
20. Comisia Europeană
21. Európskej komisii
22. Evropski komisiji
23. Euroopan komission
24. Europeiska kommissionen
25. EC
26. EU
Awards2
1. BMH4-CT96-0249
2. QLRT-1999-31556

BibTeX

@article{Buzanska_2001, title={Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells}, volume={65}, ISSN={1097-4547}, url={http://dx.doi.org/10.1002/jnr.1123}, DOI={10.1002/jnr.1123}, number={1}, journal={Journal of Neuroscience Research}, publisher={Wiley}, author={Buzanska, L. and Spassky, N. and Belin, M.F. and Giangrande, A. and Guillemot, F. and Klämbt, C. and Labouesse, M. and Thomas, J.L. and Domanska‐Janik, K. and Zalc, B.}, year={2001}, month=jun, pages={17–23} }

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