DOI: 10.1002/jmv.10245. Long‐term survival and virus production in human primary macrophages infected by human immunodeficiency virus

Long‐term survival and virus production in human primary macrophages infected by human immunodeficiency virus

10.1002/jmv.10245

Crossref journal-article

Wiley

Journal of Medical Virology (311)

Abstract

AbstractThe role of macrophages in the pathogenesis and progression of human immunodeficiency virus (HIV)‐related infection is substantiated by in vitro and in vivo evidence. The unique ability to survive HIV infection and produce viral particles for long periods is postulated. Detailed studies of this phenomenon are lacking. The dynamics of HIV‐1 replication and cumulative virus production was studied in long‐term cultures of macrophages in the presence or in the absence of antiviral drugs. Multiply spliced and unspliced HIV‐RNA production was assessed by quantitative PCR, and the number of infected cells was monitored by FACS analysis. Cumulative HIV‐1 production was determined by a trapezoidal equation, including such parameters as times of collection and experimental values of genomic‐RNA and p24 gag antigen. Unspliced and multiply spliced HIV‐RNA increased linearly after macrophage infection; reached levels of 1.5 × 108 and 2.8 × 105 copies/105 cells, respectively, at day 10; and then remained stable throughout the course of the experiment. Cumulative production of genomic‐RNA and p24 gag antigen was 1010 copies/106 cells and 107 pg/106 cells, respectively, with an average of >200 virus particles produced daily by each macrophage. AZT decreased the cumulative production of both genomic‐RNA and p24 gag antigen down to 2.5 × 109 copies and 1.1 × 106 pg/106 cells (73.8% and 88.9% inhibition, respectively) up to day 50 without virus breakthrough. Ritonavir had a limited, but consistent, efficacy on the release of mature virus proteins (about 40% inhibition), but not on HIV‐RNA production. In conclusion, the long‐term dynamics and the high cumulative virus production that characterize HIV‐1 infection of macrophages underscore the peculiar role of these cells as a persistently infected reservoir of HIV. J. Med. Virol. 68:479–488, 2002. © 2002 Wiley‐Liss, Inc.

Bibliography

Aquaro, S., Bagnarelli, P., Guenci, T., De Luca, A., Clementi, M., Balestra, E., CaliÃ², R., & Perno, C. (2002). Longâterm survival and virus production in human primary macrophages infected by human immunodeficiency virus. Journal of Medical Virology, 68(4), 479â488. Portico.

Authors 8

Stefano Aquaro (first)
Patrizia Bagnarelli (additional)
Tania Guenci (additional)
Andrea De Luca (additional)
Massimo Clementi (additional)
Emanuela Balestra (additional)
Raffaele Caliò (additional)
Carlo‐Federico Perno (additional)

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Dates

Type	When
Created	22 years, 10 months ago (Oct. 11, 2002, 6:29 p.m.)
Deposited	1 year, 9 months ago (Nov. 18, 2023, 3:01 a.m.)
Indexed	1 year ago (Sept. 6, 2024, 7:43 a.m.)
Issued	22 years, 10 months ago (Oct. 10, 2002)
Published	22 years, 10 months ago (Oct. 10, 2002)
Published Online	22 years, 10 months ago (Oct. 10, 2002)
Published Print	22 years, 9 months ago (Dec. 1, 2002)

Funders 5

Italian National Institute of Health (ISS; Project AIDS)
European Community
National Research Council (CNR)
MURST
Ricerca Finalizzata and Corrente of the Ministry of Health

BibTeX

@article{Aquaro_2002, title={Long‐term survival and virus production in human primary macrophages infected by human immunodeficiency virus}, volume={68}, ISSN={1096-9071}, url={http://dx.doi.org/10.1002/jmv.10245}, DOI={10.1002/jmv.10245}, number={4}, journal={Journal of Medical Virology}, publisher={Wiley}, author={Aquaro, Stefano and Bagnarelli, Patrizia and Guenci, Tania and De Luca, Andrea and Clementi, Massimo and Balestra, Emanuela and Caliò, Raffaele and Perno, Carlo‐Federico}, year={2002}, month=oct, pages={479–488} }

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