DOI: 10.1002/jms.1190300803. Determining calcium‐binding stoichiometry and cooperativity of parvalbumin and calmodulin by mass spectrometry

Determining calcium‐binding stoichiometry and cooperativity of parvalbumin and calmodulin by mass spectrometry

10.1002/jms.1190300803

Crossref journal-article

Wiley

Journal of Mass Spectrometry (311)

Abstract

AbstractThe calcium‐binding properties of parvalbumin and calmodulin were investigated by electrospray ionization mass spectrometry (ESI‐MS). The two calcium sites of parvalbumin were found to be strongly cooperative in binding Ca2+ ion. Up to four calcium ions were found to bind to calmodulin at high calcium concentration levels. Strong cooperativity was detected between the third and fourth Ca2+ binding sites of calmodulin (ordered by loading sequence). Strong interactions were also indicated between the two halves of the calmodulin molecule. Demetallation of the gas‐phase ions can occur during the desolvation process, especially for ESI of aqueous solutions. However, the ESI‐MS methodology has the potential to provide insight into the intricate processes involved in metal site communications for other metalloproteins.

Bibliography

Hu, P., & Loo, J. A. (1995). Determining calciumâbinding stoichiometry and cooperativity of parvalbumin and calmodulin by mass spectrometry. Journal of Mass Spectrometry, 30(8), 1076â1082. Portico.

Authors 2

Peifeng Hu (first)
Joseph A. Loo (additional)

References 39 Referenced 41

{'key': 'e_1_2_1_2_2', 'first-page': '1', 'volume-title': 'Advances in Protein Chemistry', 'author': 'Glusker J. P.', 'year': '1991'} / Advances in Protein Chemistry by Glusker J. P. (1991)
10.1021/bi00483a023
10.1021/bi00409a036
10.1126/science.2675315
10.1016/0003-2697(89)90153-X
10.1126/science.1411504
10.1002/bms.1200220902
10.1002/jms.1190300127
10.1021/ac00058a010
10.1042/bj2960657
10.1016/0014-5793(92)81115-3
10.1021/ac00095a013
10.1002/oms.1210281241
10.1021/ac00093a020
10.1002/mas.1280090103
10.1021/bi00340a005
10.1021/bi00409a037 / Biochemistry by Costelli F. (1988)
{'key': 'e_1_2_1_19_2', 'first-page': '35', 'volume-title': 'Calmodulin, Molecular Aspects of Cellular Regulation', 'author': 'Klee C. B.', 'year': '1988'} / Calmodulin, Molecular Aspects of Cellular Regulation by Klee C. B. (1988)
{'key': 'e_1_2_1_20_2', 'volume-title': 'The History of Calcium‐Binding Proteins', 'author': 'Dieter P.', 'year': '1988'} / The History of Calcium‐Binding Proteins by Dieter P. (1988)
{'key': 'e_1_2_1_21_2', 'first-page': '77', 'volume-title': 'Advances in Protein Chemistry', 'author': 'McPhalen C. A.', 'year': '1991'} / Advances in Protein Chemistry by McPhalen C. A. (1991)
10.1016/S0021-9258(19)42523-4
10.1073/pnas.69.3.581
10.1016/S0300-9084(79)80158-3
10.1021/bi00719a016
10.1111/j.1432-1033.1980.tb06076.x
10.1111/j.1432-1033.1980.tb04796.x
10.1016/S0021-9258(19)36871-1
10.1021/bi00355a057
10.1021/bi00580a010
10.1021/bi00340a006
10.1016/0301-4622(84)87009-X
10.1016/0301-4622(87)80093-5
10.1021/bi00452a028
10.1021/bi00056a015 / Biochemistry by Blancuzzi Y. (1992)
10.1038/newbio240085a0
10.1146/annurev.bi.58.070189.004511
10.1021/bi00304a013
10.1016/0006-291X(85)90434-6
{'key': 'e_1_2_1_40_2', 'first-page': '97', 'volume-title': 'Calcium‐Binding Proteins', 'author': 'Wang C.‐L. A.', 'year': '1988'} / Calcium‐Binding Proteins by Wang C.‐L. A. (1988)

Dates

Type	When
Created	20 years, 2 months ago (May 31, 2005, 7:52 a.m.)
Deposited	1 year, 9 months ago (Oct. 25, 2023, 2:56 p.m.)
Indexed	3 months, 1 week ago (May 13, 2025, 12:07 a.m.)
Issued	30 years ago (Aug. 1, 1995)
Published	30 years ago (Aug. 1, 1995)
Published Online	20 years, 4 months ago (April 14, 2005)
Published Print	30 years ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Hu_1995, title={Determining calcium‐binding stoichiometry and cooperativity of parvalbumin and calmodulin by mass spectrometry}, volume={30}, ISSN={1096-9888}, url={http://dx.doi.org/10.1002/jms.1190300803}, DOI={10.1002/jms.1190300803}, number={8}, journal={Journal of Mass Spectrometry}, publisher={Wiley}, author={Hu, Peifeng and Loo, Joseph A.}, year={1995}, month=aug, pages={1076–1082} }

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