Antibody affinity measurements
10.1002/jmr.300030304
Crossref journal-article
Wiley
Journal of Molecular Recognition (311)
Abstract

AbstractThe use of antibodies in immunoaffinity separations represents one of the most specific methods for purifying substances of biological interest. Since the binding affinity of antibody greatly influences its behavior in such separations, it is often important to know the value of the antibody affinity expressed as an equilibrium constant K. The present review discussed the equations used in the quantitative analysis of antigen/antibody interactions and describes currently used experimental methods for measuring K values. Advantages and shortcomings of the solution phase and solid phase approaches used for measuring antibody affinity are discussed.

Bibliography

Azimzadeh, A., & Van Regenmortel, M. H. V. (1990). Antibody affinity measurements. Journal of Molecular Recognition, 3(3), 108–116. Portico.

Authors 2
  1. A. Azimzadeh (first)
  2. M. H. V. Van Regenmortel (additional)
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Dates
Type When
Created 20 years, 2 months ago (May 29, 2005, 6:27 a.m.)
Deposited 1 year, 11 months ago (Sept. 27, 2023, 10:02 a.m.)
Indexed 1 year ago (Aug. 4, 2024, 8:57 p.m.)
Issued 35 years, 2 months ago (June 1, 1990)
Published 35 years, 2 months ago (June 1, 1990)
Published Online 21 years, 6 months ago (Feb. 3, 2004)
Published Print 35 years, 2 months ago (June 1, 1990)
Funders 0

None

@article{Azimzadeh_1990, title={Antibody affinity measurements}, volume={3}, ISSN={1099-1352}, url={http://dx.doi.org/10.1002/jmr.300030304}, DOI={10.1002/jmr.300030304}, number={3}, journal={Journal of Molecular Recognition}, publisher={Wiley}, author={Azimzadeh, A. and Van Regenmortel, M. H. V.}, year={1990}, month=jun, pages={108–116} }