DOI: 10.1002/jez.1402470108. Treatment of brachial nerves with colchicine inhibits limb regeneration in the newt <i>Notophthalmus viridescens</i>

Treatment of brachial nerves with colchicine inhibits limb regeneration in the newt Notophthalmus viridescens

10.1002/jez.1402470108

Crossref journal-article

Wiley

Journal of Experimental Zoology (311)

Abstract

AbstractIn urodele amphibians, limb regeneration is dependent on innervation and is blocked by the administration of colchicine. The objective of this experiment was to determine if colchicine blocks limb regeneration by a direct action on the blastema cells or by an indirect action on the nerves, specifically, if colchicine treatment of the brachial nerves would inhibit limb regeneration in the newt Notophthalmus viridescens. Colchicine was applied to the nerves by implanting a colchicine‐loaded silastin block adjacent to the brachial nerves of an amputated newt limb. With appropriate dose levels of colchicine, limb regeneration was completely inhibited. Contralateral control limbs, carrying unloaded silastin blocks, and control limbs with colchicine‐loaded blocks implanted equidistant from the blastema, but not adjacent to the brachial nerves, regenerated normally. Thus, the results indicate that the colchicine inhibition of limb regeneration is mediated by colchicine effects on the nerves. The possible mechanism of colchicine action on nerves may involve either wallerian degeneration, or inhibition of axoplasmic transport, or both.

Bibliography

Scadding, S. R. (1988). Treatment of brachial nerves with colchicine inhibits limb regeneration in the newt Notophthalmus viridescens. Journal of Experimental Zoology, 247(1), 56â61. Portico.

Authors 1

Steven R. Scadding (first)

References 33 Referenced 4

10.1242/jeb.132.1.79 / J. Exp. Biol. / The nerve dependence of amphibian limb regeneration by Brockes J. P. (1987)
10.1139/z83-074
10.1007/978-3-642-96436-7
10.1002/ar.1092040206
10.1093/icb/18.4.855
{'key': 'e_1_2_1_6_1', 'first-page': '106', 'volume-title': 'Regulation of Vertebrate Limb Regeneration', 'author': 'Globus M.', 'year': '1985'} / Regulation of Vertebrate Limb Regeneration by Globus M. (1985)
{'key': 'e_1_2_1_7_1', 'first-page': '373', 'article-title': 'Mitosis inhibitors and axonal transport', 'volume': '7', 'author': 'Hanson M.', 'year': '1978', 'journal-title': 'Int. Rev. Cytol. [Suppl.]'} / Int. Rev. Cytol. [Suppl.] / Mitosis inhibitors and axonal transport by Hanson M. (1978)
{'key': 'e_1_2_1_8_1', 'volume-title': 'Animal Tissue Techniques', 'author': 'Humason G. L.', 'year': '1979'} / Animal Tissue Techniques by Humason G. L. (1979)
10.1007/BF00575834
10.1083/jcb.52.3.719
{'key': 'e_1_2_1_11_1', 'first-page': '1', 'article-title': 'Effects of delayed amputation on denervated forelimbs of adult newt', 'volume': '75', 'author': 'Liversage R. A.', 'year': '1983', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Effects of delayed amputation on denervated forelimbs of adult newt by Liversage R. A. (1983)
10.1002/jez.1401700402
10.1007/BF00848251
10.1016/0006-8993(84)91105-3
{'key': 'e_1_2_1_15_1', 'first-page': '183', 'article-title': 'Neurotrophic control of events in injured forelimbs of larval urodeles', 'volume': '69', 'author': 'Mescher A. L.', 'year': '1982', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Neurotrophic control of events in injured forelimbs of larval urodeles by Mescher A. L. (1982)
10.1002/jez.1402290210
{'key': 'e_1_2_1_17_1', 'first-page': '275', 'article-title': 'The role of size in the rate of limb regeneration in the adult newt', 'volume': '36', 'author': 'Pritchett W. H.', 'year': '1972', 'journal-title': 'Growth'} / Growth / The role of size in the rate of limb regeneration in the adult newt by Pritchett W. H. (1972)
10.1139/z83-070
10.1002/jez.1402150208
10.1139/z81-007
10.1002/jez.1402290116
10.1007/BF01920655
{'key': 'e_1_2_1_23_1', 'first-page': '55', 'article-title': 'The effects of local application of retinoic acid on limb development and regeneration in tadpoles of Xenopus laevis', 'volume': '91', 'author': 'Scadding S. R.', 'year': '1986', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / The effects of local application of retinoic acid on limb development and regeneration in tadpoles of Xenopus laevis by Scadding S. R. (1986)
10.1016/0303-2647(83)90026-6
10.1111/j.1749-6632.1974.tb20520.x
10.1093/icb/18.4.829
10.1002/jez.1401310306
10.1002/aja.1001330105
{'key': 'e_1_2_1_29_1', 'first-page': '81', 'volume-title': 'Regulation of Vertebrate Limb Regeneration', 'author': 'Tassava R. A.', 'year': '1985'} / Regulation of Vertebrate Limb Regeneration by Tassava R. A. (1985)
{'key': 'e_1_2_1_30_1', 'first-page': '537', 'volume-title': 'Limb Development and Regeneration, Part A', 'author': 'Tassava R. A.', 'year': '1983'} / Limb Development and Regeneration, Part A by Tassava R. A. (1983)
10.1002/jez.1400920304
10.1007/BF02618075
{'key': 'e_1_2_1_33_1', 'volume-title': 'Vertebrate Limb Regeneration', 'author': 'Wallace H.', 'year': '1981'} / Vertebrate Limb Regeneration by Wallace H. (1981)

Dates

Type	When
Created	20 years, 2 months ago (June 11, 2005, 8:56 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 1:42 a.m.)
Indexed	1 year, 9 months ago (Oct. 27, 2023, 4:42 p.m.)
Issued	37 years, 1 month ago (July 1, 1988)
Published	37 years, 1 month ago (July 1, 1988)
Published Online	20 years, 4 months ago (April 25, 2005)
Published Print	37 years, 1 month ago (July 1, 1988)

Funders 0

None

BibTeX

@article{Scadding_1988, title={Treatment of brachial nerves with colchicine inhibits limb regeneration in the newt Notophthalmus viridescens}, volume={247}, ISSN={1097-010X}, url={http://dx.doi.org/10.1002/jez.1402470108}, DOI={10.1002/jez.1402470108}, number={1}, journal={Journal of Experimental Zoology}, publisher={Wiley}, author={Scadding, Steven R.}, year={1988}, month=jul, pages={56–61} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        27
      ]
    ],
    "date-time": "2023-10-27T20:42:18Z",
    "timestamp": 1698439338543
  },
  "reference-count": 33,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            4,
            25
          ]
        ],
        "date-time": "2005-04-25T00:00:00Z",
        "timestamp": 1114387200000
      },
      "content-version": "vor",
      "delay-in-days": 6142,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1988,
        7
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>In urodele amphibians, limb regeneration is dependent on innervation and is blocked by the administration of colchicine. The objective of this experiment was to determine if colchicine blocks limb regeneration by a direct action on the blastema cells or by an indirect action on the nerves, specifically, if colchicine treatment of the brachial nerves would inhibit limb regeneration in the newt <jats:italic>Notophthalmus viridescens</jats:italic>. Colchicine was applied to the nerves by implanting a colchicine\u2010loaded silastin block adjacent to the brachial nerves of an amputated newt limb. With appropriate dose levels of colchicine, limb regeneration was completely inhibited. Contralateral control limbs, carrying unloaded silastin blocks, and control limbs with colchicine\u2010loaded blocks implanted equidistant from the blastema, but not adjacent to the brachial nerves, regenerated normally. Thus, the results indicate that the colchicine inhibition of limb regeneration is mediated by colchicine effects on the nerves. The possible mechanism of colchicine action on nerves may involve either wallerian degeneration, or inhibition of axoplasmic transport, or both.</jats:p>",
  "DOI": "10.1002/jez.1402470108",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        6,
        11
      ]
    ],
    "date-time": "2005-06-11T12:56:08Z",
    "timestamp": 1118494568000
  },
  "page": "56-61",
  "source": "Crossref",
  "is-referenced-by-count": 4,
  "title": "Treatment of brachial nerves with colchicine inhibits limb regeneration in the newt <i>Notophthalmus viridescens</i>",
  "prefix": "10.1002",
  "volume": "247",
  "author": [
    {
      "given": "Steven R.",
      "family": "Scadding",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        4,
        25
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1242/jeb.132.1.79",
      "article-title": "The nerve dependence of amphibian limb regeneration",
      "volume": "132",
      "author": "Brockes J. P.",
      "year": "1987",
      "journal-title": "J. Exp. Biol."
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1139/z83-074"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-642-96436-7"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ar.1092040206"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/icb/18.4.855"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "106",
      "volume-title": "Regulation of Vertebrate Limb Regeneration",
      "author": "Globus M.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "373",
      "article-title": "Mitosis inhibitors and axonal transport",
      "volume": "7",
      "author": "Hanson M.",
      "year": "1978",
      "journal-title": "Int. Rev. Cytol. [Suppl.]"
    },
    {
      "key": "e_1_2_1_8_1",
      "volume-title": "Animal Tissue Techniques",
      "author": "Humason G. L.",
      "year": "1979"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00575834"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.52.3.719"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "1",
      "article-title": "Effects of delayed amputation on denervated forelimbs of adult newt",
      "volume": "75",
      "author": "Liversage R. A.",
      "year": "1983",
      "journal-title": "J. Embryol. Exp. Morphol."
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1401700402"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00848251"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(84)91105-3"
    },
    {
      "key": "e_1_2_1_15_1",
      "first-page": "183",
      "article-title": "Neurotrophic control of events in injured forelimbs of larval urodeles",
      "volume": "69",
      "author": "Mescher A. L.",
      "year": "1982",
      "journal-title": "J. Embryol. Exp. Morphol."
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1402290210"
    },
    {
      "key": "e_1_2_1_17_1",
      "first-page": "275",
      "article-title": "The role of size in the rate of limb regeneration in the adult newt",
      "volume": "36",
      "author": "Pritchett W. H.",
      "year": "1972",
      "journal-title": "Growth"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1139/z83-070"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1402150208"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1139/z81-007"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1402290116"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01920655"
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "55",
      "article-title": "The effects of local application of retinoic acid on limb development and regeneration in tadpoles of Xenopus laevis",
      "volume": "91",
      "author": "Scadding S. R.",
      "year": "1986",
      "journal-title": "J. Embryol. Exp. Morphol."
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0303-2647(83)90026-6"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1749-6632.1974.tb20520.x"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/icb/18.4.829"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1401310306"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/aja.1001330105"
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "81",
      "volume-title": "Regulation of Vertebrate Limb Regeneration",
      "author": "Tassava R. A.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "537",
      "volume-title": "Limb Development and Regeneration, Part A",
      "author": "Tassava R. A.",
      "year": "1983"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jez.1400920304"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02618075"
    },
    {
      "key": "e_1_2_1_33_1",
      "volume-title": "Vertebrate Limb Regeneration",
      "author": "Wallace H.",
      "year": "1981"
    }
  ],
  "container-title": "Journal of Experimental Zoology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402470108",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402470108",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T05:42:20Z",
    "timestamp": 1697953340000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jez.1402470108"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1988,
        7
      ]
    ]
  },
  "references-count": 33,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1988,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jez.1402470108"
  ],
  "URL": "http://dx.doi.org/10.1002/jez.1402470108",
  "relation": {},
  "ISSN": [
    "0022-104X",
    "1097-010X"
  ],
  "subject": [],
  "container-title-short": "J. Exp. Zool.",
  "published": {
    "date-parts": [
      [
        1988,
        7
      ]
    ]
  }
}