DOI: 10.1002/jez.1402260220. Increase in mitotic activity of regenerating axolotl limbs by growth factor‐impregnated implants

Increase in mitotic activity of regenerating axolotl limbs by growth factor‐impregnated implants

10.1002/jez.1402260220

Crossref journal-article

Wiley

Journal of Experimental Zoology (311)

Abstract

AbstractThis study was designed to investigate the effects of pituitary fibroblast growth factor (FGF), endothelial cell growth supplement (ECGS), and human serum albumin (HSA) on the mitotic indices of dedifferentiating stump cells in regenerating limbs deprived of a wound epidermis. These cells would normally contribute to blastema cell formation but have been prevented from doing so by the placement of a full thickness skin flap over the amputation site. FGF, ECGS, and HSA were released from copolymer implants inserted between full thickness skin flaps and tibia‐fibula amputation sites in axolotls, Ambystoma mexicanum. The implants were plain or contained either FGF or ECGS or HSA. After 2 wk the mitotic indices of the area of dedifferentiation within these experimental limbs were determined and compared to the mitotic indices of normal regeneration blastemas. Histological analysis revealed that the technique of suturing the full thickness skin flaps into position prevented the formation of dermis‐free areas that can permit, regeneration. Full thickness skin flaps alone, or with a plain implant, or with an HSA‐impregnated implant completely inhibited blastema formation in axolotl hindlimbs. The mitotic indices of these animals showed significant decreases compared to mitotic indices of normal regeneration blastemas. The mitotic indices of animals treated with full thickness skin flaps and implants impregnated with either FGF or ECGS showed significant increases compared to those of normal regeneration blastemas. Precocious cartilage development was found in limbs which contained FGF implants.

Bibliography

Chew, K. E., & Cameron, J. A. (1983). Increase in mitotic activity of regenerating axolotl limbs by growth factorâimpregnated implants. Journal of Experimental Zoology, 226(2), 325â329. Portico.

Authors 2

Keith E. Chew (first)
Jo Ann Cameron (additional)

References 15 Referenced 26

{'key': 'e_1_2_1_2_1', 'first-page': '37', 'volume-title': 'Levels of Genetic Control in Development: 39th Symposium of the Soc. for Devel. Biol.', 'author': 'Caplan A. I.', 'year': '1981'} / Levels of Genetic Control in Development: 39th Symposium of the Soc. for Devel. Biol. by Caplan A. I. (1981)
10.1016/B978-0-12-027308-9.50026-3
10.1038/263797a0
10.1073/pnas.76.11.5674
10.1002/jez.1401950111
10.1002/jez.1402070318
{'key': 'e_1_2_1_8_1', 'first-page': '277', 'volume-title': 'Growth in Living Systems', 'author': 'Singer M.', 'year': '1961'} / Growth in Living Systems by Singer M. (1961)
{'key': 'e_1_2_1_9_1', 'first-page': '187', 'article-title': 'Growth factors and pattern formation', 'volume': '65', 'author': 'Smith J. C.', 'year': '1981', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Growth factors and pattern formation by Smith J. C. (1981)
10.1002/jez.1402120210
10.1002/jmor.1051500106
10.1002/jez.1402080111
{'key': 'e_1_2_1_13_1', 'first-page': '49', 'article-title': 'Injury requirement for initiation of regeneration of newt limbs which have whole skin grafts', 'volume': '28', 'author': 'Tassava R. A.', 'year': '1979', 'journal-title': 'Nature'} / Nature / Injury requirement for initiation of regeneration of newt limbs which have whole skin grafts by Tassava R. A. (1979)
10.1111/j.1432-0436.1975.tb01439.x
10.1016/B978-1-4831-9954-2.50010-0
10.1242/jcs.20.3.539 / J. Cell. Sci. / The cell cycle during amphibian limb regeneration by Wallace H. (1976)

Dates

Type	When
Created	20 years, 2 months ago (June 11, 2005, 1:20 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 5:29 a.m.)
Indexed	1 year, 7 months ago (Jan. 2, 2024, 5:23 a.m.)
Issued	42 years, 4 months ago (May 1, 1983)
Published	42 years, 4 months ago (May 1, 1983)
Published Online	20 years, 3 months ago (June 1, 2005)
Published Print	42 years, 4 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Chew_1983, title={Increase in mitotic activity of regenerating axolotl limbs by growth factor‐impregnated implants}, volume={226}, ISSN={1097-010X}, url={http://dx.doi.org/10.1002/jez.1402260220}, DOI={10.1002/jez.1402260220}, number={2}, journal={Journal of Experimental Zoology}, publisher={Wiley}, author={Chew, Keith E. and Cameron, Jo Ann}, year={1983}, month=may, pages={325–329} }

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