DOI: 10.1002/jez.1402060102. Effects of continual mechanical agitation prior to cleavage in echinoderm eggs

Effects of continual mechanical agitation prior to cleavage in echinoderm eggs

10.1002/jez.1402060102

Crossref journal-article

Wiley

Journal of Experimental Zoology (311)

Abstract

AbstractThe force necessary to accomplish animal cell division is exerted by a ring of specialized cytoplasm which is rapidly established at the equatorial surface by the mitotic apparatus. Although the structure of the cleavage mechanism is not demonstrable before it is functional, the geometrical relation between the mitotic apparatus and the equatorial surface is established early in the mitotic cycle and remains unchanged. In order to learn more about the process by which the mitotic apparatus establishes the division mechanism, regions of cleaving echinoderm eggs (Echinarachnius parma, Lytechinus pictus) were continually agitated or stretched while establishment took place. Normal cleavage occurred despite stroking and vibration of the equatorial surface at different frequencies. Cells continually stretched at right angles to the future cleavage plane produced temporally and morphologically normal furrows. Subsurface cytoplasm was agitated by moving the mitotic apparatus back and forth parallel to its axis and by a vibrating needle inserted between the mitotic apparatus and the equatorial margin of flattened cells. Neither of these operations blocked cleavage. Cells held and shaped by the nozzle of a pipette were pierced by a needle inserted through the polar surface and into the zone between the mitotic apparatus and the equatorial surface. The needle was then swept around the entire mitotic apparatus. Furrows developed in these cells; they appeared later than in controls and did not appear completely normal. Furrow establishment does not seem to depend upon a precise, long‐standing, stable arrangement of sub‐surface cytoplasmic components.

Bibliography

Rappaport, R. (1978). Effects of continual mechanical agitation prior to cleavage in echinoderm eggs. Journal of Experimental Zoology, 206(1), 1â11. Portico.

Authors 1

R. Rappaport (first)

References 45 Referenced 19

{'key': 'e_1_2_1_2_1', 'first-page': '408', 'article-title': 'Formation of the first cleavage furrow in a telolethical egg', 'volume': '135', 'author': 'Arnold J. M.', 'year': '1968', 'journal-title': 'Biol. Bull.'} / Biol. Bull. / Formation of the first cleavage furrow in a telolethical egg by Arnold J. M. (1968)
10.1007/BF01666538
10.1002/jez.1400230302
{'key': 'e_1_2_1_5_1', 'first-page': '297', 'article-title': 'Behavior of the cell surface during cleavage. V. Perforation experiment', 'volume': '6', 'author': 'Dan K.', 'year': '1943', 'journal-title': 'J. Fac. Sci. Tokyo Imp. Univ.'} / J. Fac. Sci. Tokyo Imp. Univ. / Behavior of the cell surface during cleavage. V. Perforation experiment by Dan K. (1943)
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{'key': 'e_1_2_1_10_1', 'first-page': '105', 'article-title': 'Aster formation in eggs of Xenopus laevis', 'volume': '67', 'author': 'Heidemann S. R.', 'year': '1975', 'journal-title': 'Induction by isolated basal bodies. J. Cell Biol.'} / Induction by isolated basal bodies. J. Cell Biol. / Aster formation in eggs of Xenopus laevis by Heidemann S. R. (1975)
{'key': 'e_1_2_1_11_1', 'first-page': '103', 'article-title': 'Recherches sur les oeufs di‐et‐trispermiques de grenouille', 'volume': '26', 'author': 'Herlant M.', 'year': '1911', 'journal-title': 'Archives de Biologie'} / Archives de Biologie / Recherches sur les oeufs di‐et‐trispermiques de grenouille by Herlant M. (1911)
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{'key': 'e_1_2_1_41_1', 'first-page': '163', 'article-title': 'The mechanics and mechanism of cleavage', 'volume': '10', 'author': 'Wolpert L.', 'year': '1960', 'journal-title': 'Internat. Rev. Cytol.'} / Internat. Rev. Cytol. / The mechanics and mechanism of cleavage by Wolpert L. (1960)
10.1126/science.20.521.889-b
{'issue': '3', 'key': 'e_1_2_1_43_1', 'first-page': '1', 'article-title': 'Observations and experiments on the ctenophore egg', 'volume': '32', 'author': 'Yatsu N.', 'year': '1912', 'journal-title': 'J. Coll. Sci. Tokyo'} / J. Coll. Sci. Tokyo / Observations and experiments on the ctenophore egg by Yatsu N. (1912)
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{'key': 'e_1_2_1_45_1', 'first-page': '33', 'article-title': 'Experimentelle Studien über die Zelltheilung. III Die Furchungszellen von Beroë ovata', 'volume': '7', 'author': 'Ziegler H. E.', 'year': '1998', 'journal-title': 'Arch. f. Entwicklungsmech.'} / Arch. f. Entwicklungsmech. / Experimentelle Studien über die Zelltheilung. III Die Furchungszellen von Beroë ovata by Ziegler H. E. (1998)
10.1007/BF02152938

Dates

Type	When
Created	20 years, 2 months ago (June 11, 2005, 12:19 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 11:16 p.m.)
Indexed	2 months, 3 weeks ago (June 7, 2025, 5:28 a.m.)
Issued	46 years, 10 months ago (Oct. 1, 1978)
Published	46 years, 10 months ago (Oct. 1, 1978)
Published Online	20 years, 3 months ago (May 16, 2005)
Published Print	46 years, 10 months ago (Oct. 1, 1978)

Funders 0

None

BibTeX

@article{Rappaport_1978, title={Effects of continual mechanical agitation prior to cleavage in echinoderm eggs}, volume={206}, ISSN={1097-010X}, url={http://dx.doi.org/10.1002/jez.1402060102}, DOI={10.1002/jez.1402060102}, number={1}, journal={Journal of Experimental Zoology}, publisher={Wiley}, author={Rappaport, R.}, year={1978}, month=oct, pages={1–11} }

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