DOI: 10.1002/jemt.22236. Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy

Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy

10.1002/jemt.22236

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

ABSTRACTThe plethora of available scientific cameras of different types challenges the biologically oriented experimenter when picking the appropriate camera for his experiment. In this study, we chose to investigate camera performances in a typical nonsingle molecule situation in life sciences, that is, quantitative measurements of fluorescence intensity changes from video data with typically skewed intensity distributions. Here, intensity profile dynamics of pH‐sensors upon triggered changes of pH‐environments in living cells served as a model system. The following camera types were tested: sCMOS, CCD (scientific and nonscientific) and EM‐CCD (back‐ and front‐illuminated). We found that although the EM‐CCD cameras achieved the best absolute spatial SNR (signal‐to‐noise ratio) values, the sCMOS was at least of equal performance when the spatial SNR was related to the effective dynamic range, and it was superior in terms of temporal SNR. In the measurements of triggered intensity changes, the sCMOS camera had the advantage that it used the smallest fraction of its dynamic range when depicting intensity changes, and thus featured the best SNR at full usage of its dynamic range. Microsc. Res. Tech. 76:835–843, 2013. © 2013 Wiley Periodicals, Inc.

Bibliography

Jung, J., Weisenburger, S., Albert, S., Gilbert, D. F., Friedrich, O., Eulenburg, V., Kornhuber, J., & Groemer, T. W. (2013). Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy. Microscopy Research and Technique, 76(8), 835â843. Portico.

Authors 8

Jasmin Jung (first)
Siegfried Weisenburger (additional)
Sahradha Albert (additional)
Daniel F. Gilbert (additional)
Oliver Friedrich (additional)
Volker Eulenburg (additional)
Johannes Kornhuber (additional)
Teja W. Groemer (additional)

References 35 Referenced 7

10.2144/02335dd10
10.1523/JNEUROSCI.12-02-00363.1992
10.1038/346818a0
10.1523/JNEUROSCI.3398-07.2008
10.1038/359859a0
{'key': 'e_1_2_6_7_1', 'volume-title': 'Live Cell Imaging: A Laboratory Manual', 'author': 'Goldman RD', 'year': '2005'} / Live Cell Imaging: A Laboratory Manual by Goldman RD (2005)
10.1063/1.3148224
10.1038/373663b0
10.1364/OE.19.019156
10.1016/j.toxlet.2012.08.027
10.1039/b807408a
10.1364/OE.20.017741
10.1186/1756-6606-3-11
10.1038/28190
10.1007/978-0-387-45524-2
10.1016/j.bbrc.2009.09.054
10.1523/JNEUROSCI.19-15-06427.1999
10.1117/1.3505017
10.1038/nmeth.1991
10.1038/35008615
10.1364/OE.20.007338
10.1016/j.jsb.2005.06.002
10.1038/nmeth.1202
10.1038/nature09642
10.1002/jemt.20858
10.1016/S0896-6273(00)80376-1
10.1038/nmeth.2062
10.1074/jbc.M109.043711
10.1002/jemt.20943
10.1111/j.1471-4159.2006.03821.x
10.1034/j.1600-0854.2002.31108.x
10.1371/journal.pone.0013514
10.1016/j.bpj.2010.12.3706
10.1007/s00249-011-0722-3
10.1038/nn1739

Dates

Type	When
Created	12 years, 3 months ago (June 3, 2013, 8 a.m.)
Deposited	1 year, 11 months ago (Oct. 3, 2023, 3:48 p.m.)
Indexed	1 year, 11 months ago (Oct. 4, 2023, 1:40 a.m.)
Issued	12 years, 3 months ago (June 3, 2013)
Published	12 years, 3 months ago (June 3, 2013)
Published Online	12 years, 3 months ago (June 3, 2013)
Published Print	12 years, 1 month ago (Aug. 1, 2013)

Funders 0

None

BibTeX

@article{Jung_2013, title={Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy}, volume={76}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.22236}, DOI={10.1002/jemt.22236}, number={8}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Jung, Jasmin and Weisenburger, Siegfried and Albert, Sahradha and Gilbert, Daniel F. and Friedrich, Oliver and Eulenburg, Volker and Kornhuber, Johannes and Groemer, Teja W.}, year={2013}, month=jun, pages={835–843} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        4
      ]
    ],
    "date-time": "2023-10-04T05:40:19Z",
    "timestamp": 1696398019768
  },
  "reference-count": 35,
  "publisher": "Wiley",
  "issue": "8",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2013,
            6,
            3
          ]
        ],
        "date-time": "2013-06-03T00:00:00Z",
        "timestamp": 1370217600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2013,
        8
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title><jats:p>The plethora of available scientific cameras of different types challenges the biologically oriented experimenter when picking the appropriate camera for his experiment. In this study, we chose to investigate camera performances in a typical nonsingle molecule situation in life sciences, that is, quantitative measurements of fluorescence intensity changes from video data with typically skewed intensity distributions. Here, intensity profile dynamics of pH\u2010sensors upon triggered changes of pH\u2010environments in living cells served as a model system. The following camera types were tested: sCMOS, CCD (scientific and nonscientific) and EM\u2010CCD (back\u2010 and front\u2010illuminated). We found that although the EM\u2010CCD cameras achieved the best absolute spatial SNR (signal\u2010to\u2010noise ratio) values, the sCMOS was at least of equal performance when the spatial SNR was related to the effective dynamic range, and it was superior in terms of temporal SNR. In the measurements of triggered intensity changes, the sCMOS camera had the advantage that it used the smallest fraction of its dynamic range when depicting intensity changes, and thus featured the best SNR at full usage of its dynamic range. <jats:italic>Microsc. Res. Tech. 76:835\u2013843, 2013</jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.</jats:p>",
  "DOI": "10.1002/jemt.22236",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        6,
        3
      ]
    ],
    "date-time": "2013-06-03T12:00:12Z",
    "timestamp": 1370260812000
  },
  "page": "835-843",
  "source": "Crossref",
  "is-referenced-by-count": 7,
  "title": "Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy",
  "prefix": "10.1002",
  "volume": "76",
  "author": [
    {
      "given": "Jasmin",
      "family": "Jung",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Psychiatry and Psychotherapy Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91054 Germany"
        }
      ]
    },
    {
      "given": "Siegfried",
      "family": "Weisenburger",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Nano\u2010Optics Division, Max Planck Institute for the Science of Light Erlangen 91058 Germany"
        }
      ]
    },
    {
      "given": "Sahradha",
      "family": "Albert",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Psychiatry and Psychotherapy Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91054 Germany"
        }
      ]
    },
    {
      "given": "Daniel F.",
      "family": "Gilbert",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Medical Biotechnology Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91052 Germany"
        }
      ]
    },
    {
      "given": "Oliver",
      "family": "Friedrich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Medical Biotechnology Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91052 Germany"
        }
      ]
    },
    {
      "given": "Volker",
      "family": "Eulenburg",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Molecular Medicine Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91054 Germany"
        }
      ]
    },
    {
      "given": "Johannes",
      "family": "Kornhuber",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Psychiatry and Psychotherapy Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91054 Germany"
        }
      ]
    },
    {
      "given": "Teja W.",
      "family": "Groemer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Psychiatry and Psychotherapy Friedrich\u2010Alexander\u2010University of Erlangen\u2010Nuremberg Erlangen 91054 Germany"
        }
      ]
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2013,
        6,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.2144/02335dd10"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.12-02-00363.1992"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/346818a0"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.3398-07.2008"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/359859a0"
    },
    {
      "key": "e_1_2_6_7_1",
      "volume-title": "Live Cell Imaging: A Laboratory Manual",
      "author": "Goldman RD",
      "year": "2005"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.3148224"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/373663b0"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.19.019156"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.toxlet.2012.08.027"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1039/b807408a"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.20.017741"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1756-6606-3-11"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/28190"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-0-387-45524-2"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbrc.2009.09.054"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.19-15-06427.1999"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1117/1.3505017"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1991"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35008615"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.20.007338"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2005.06.002"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1202"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature09642"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.20858"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80376-1"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.2062"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M109.043711"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.20943"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.2006.03821.x"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1034/j.1600-0854.2002.31108.x"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pone.0013514"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bpj.2010.12.3706"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00249-011-0722-3"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nn1739"
    }
  ],
  "container-title": "Microscopy Research and Technique",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.22236",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://onlinelibrary.wiley.com/wol1/doi/10.1002/jemt.22236/fullpdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        3
      ]
    ],
    "date-time": "2023-10-03T19:48:26Z",
    "timestamp": 1696362506000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.22236"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2013,
        6,
        3
      ]
    ]
  },
  "references-count": 35,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          2013,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jemt.22236"
  ],
  "URL": "http://dx.doi.org/10.1002/jemt.22236",
  "relation": {},
  "ISSN": [
    "1059-910X",
    "1097-0029"
  ],
  "subject": [],
  "container-title-short": "Microscopy Res &amp; Technique",
  "published": {
    "date-parts": [
      [
        2013,
        6,
        3
      ]
    ]
  }
}