DOI: 10.1002/jemt.20408. Contribution of high‐resolution correlative imaging techniques in the study of the liver sieve in three‐dimensions

Contribution of high‐resolution correlative imaging techniques in the study of the liver sieve in three‐dimensions

10.1002/jemt.20408

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

AbstractCorrelative microscopy has become increasingly important for the analysis of the structure, function, and dynamics of cells. This is largely due to the result of recent advances in light‐, probe‐, laser‐ and various electron microscopy techniques that facilitate three‐dimensional studies. Furthermore, the improved understanding in the past decade of imaging cell compartments in the third dimension has resulted largely from the availability of powerful computers, fast high‐resolution CCD cameras, specifically developed imaging analysis software, and various probes designed for labeling living and or fixed cells. In this paper, we review different correlative high‐resolution imaging methodologies and how these microscopy techniques facilitated the accumulation of new insights in the morpho‐functional and structural organization of the hepatic sieve. Various aspects of hepatic endothelial fenestrae regarding their structure, origin, dynamics, and formation will be explored throughout this paper by comparing the results of confocal laser scanning‐, correlative fluorescence and scanning electron‐, atomic force‐, and whole‐mount electron microscopy. Furthermore, the recent advances of vitrifying cells with the vitrobot in combination with the glove box for the preparation of cells for cryo‐electron microscopic investigation will be discussed. Finally, the first transmission electron tomography data of the liver sieve in three‐dimensions are presented. The obtained data unambiguously show the involvement of special domains in the de novo formation and disappearance of hepatic fenestrae, and focuses future research into the (supra)molecular structure of the fenestrae‐forming center, defenestration center and fenestrae‐, and sieve plate cytoskeleton ring by using advanced cryo‐electron tomography. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

Bibliography

Braet, F., Wisse, E., Bomans, P., Frederik, P., Geerts, W., Koster, A., Soon, L., & Ringer, S. (2007). Contribution of highâresolution correlative imaging techniques in the study of the liver sieve in threeâdimensions. Microscopy Research and Technique, 70(3), 230â242. Portico.

Authors 8

Filip Braet (first)
Eddie Wisse (additional)
Paul Bomans (additional)
Peter Frederik (additional)
Willie Geerts (additional)
Abraham Koster (additional)
Lilian Soon (additional)
Simon Ringer (additional)

References 56 Referenced 102

10.1016/j.tice.2003.11.001
10.1016/j.jsb.2004.03.010
10.1091/mbc.E02-05-0308
10.1002/jemt.1060100111
{'key': 'e_1_2_1_6_1', 'first-page': '117', 'article-title': 'Problems associated with preparations of whole mounts of cytoskeletons for high resolution electron microscopy', 'volume': '3', 'author': 'Bell PB', 'year': '1989', 'journal-title': 'Scan Electron Microsc'} / Scan Electron Microsc / Problems associated with preparations of whole mounts of cytoskeletons for high resolution electron microscopy by Bell PB (1989)
10.1111/j.1478-3231.2004.0974.x
10.1111/j.1523-1755.2005.00578_6.x
10.1186/1476-5926-1-1
{'key': 'e_1_2_1_10_1', 'first-page': '201', 'volume-title': 'Atomic force microscopy: Methods and protocols in biomedical applications', 'author': 'Braet F', 'year': '2004'} / Atomic force microscopy: Methods and protocols in biomedical applications by Braet F (2004)
{'key': 'e_1_2_1_11_1', 'first-page': '944', 'article-title': 'Assessment of a method of isolation, purification and cultivation of rat liver sinusoidal endothelial cells', 'volume': '70', 'author': 'Braet F', 'year': '1994', 'journal-title': 'Lab Invest'} / Lab Invest / Assessment of a method of isolation, purification and cultivation of rat liver sinusoidal endothelial cells by Braet F (1994)
10.1002/hep.1840210130
10.1046/j.1365-2818.1996.72348.x
10.1046/j.1365-2818.1997.1940755.x
10.1002/(SICI)1098-1098(1997)8:2<162::AID-IMA3>3.0.CO;2-8
10.1007/s003390051204 / Appl Phys A / Comparison of fixed and living liver endothelial cells by atomic force microscopy by Braet F (1998)
10.1073/pnas.95.23.13635
10.1093/jmicro/50.4.283
{'key': 'e_1_2_1_19_1', 'first-page': '437', 'volume-title': 'The liver: Biology and pathobiology', 'author': 'Braet F', 'year': '2001'} / The liver: Biology and pathobiology by Braet F (2001)
10.1186/1471-2121-3-7
10.1046/j.1365-2818.2003.01229.x
10.1053/jhep.2003.50347
10.1002/jemt.20065
10.1016/S1047-8477(02)00019-9
10.1002/hep.1840210337
10.1016/S0076-6879(05)91024-0
10.1111/j.1365-2818.1991.tb03088.x
{'key': 'e_1_2_1_28_1', 'first-page': 'S43', 'article-title': 'The ultrastructure of cryo‐sections and intact vitrified cells the effects of cryoprotectants and acceleration voltage on beam induced bubbling', 'volume': '5', 'author': 'Frederik PM', 'year': '1991', 'journal-title': 'Scan Microsc'} / Scan Microsc / The ultrastructure of cryo‐sections and intact vitrified cells the effects of cryoprotectants and acceleration voltage on beam induced bubbling by Frederik PM (1991)
{'key': 'e_1_2_1_29_1', 'first-page': '476', 'volume-title': 'Cells of the hepatic sinusoid 6', 'author': 'Frederik PM', 'year': '1997'} / Cells of the hepatic sinusoid 6 by Frederik PM (1997)
{'key': 'e_1_2_1_30_1', 'first-page': 'B383', 'volume-title': 'Proceedings of the 12th European congress on electron microscopy', 'author': 'Frederik P', 'year': '2000'} / Proceedings of the 12th European congress on electron microscopy by Frederik P (2000)
{'key': 'e_1_2_1_31_1', 'first-page': '715', 'volume-title': 'Protein‐protein interactions: A molecular cloning manual', 'author': 'Geerts WJC', 'year': '2006'} / Protein‐protein interactions: A molecular cloning manual by Geerts WJC (2006)
10.1038/nmeth806
10.1016/S0301-4622(02)00307-1
10.1111/j.0022-2720.2005.01428.x
10.1007/BF02990514
{'key': 'e_1_2_1_36_1', 'first-page': 'S6', 'article-title': 'Electron microscopy in cell biology: Integrating structure and function', 'author': 'Koster AJ', 'year': '2003', 'journal-title': 'Nat Rev Mol Cell Biol Sep'} / Nat Rev Mol Cell Biol Sep / Electron microscopy in cell biology: Integrating structure and function by Koster AJ (2003)
10.1016/j.conb.2004.08.004
10.1038/sj.gt.3302326
10.1021/a10000108
10.1002/jemt.1070220203
10.1006/jsbi.1997.3919
10.1016/S0959-4388(00)00255-5
10.1016/S0962-8924(00)01787-6
{'key': 'e_1_2_1_44_1', 'first-page': '237', 'article-title': 'Correlated confocal and intermediate voltage electron microscopy imaging of the same cells using sequential fluorescence labeling, fixation, and critical point dehydration', 'volume': '10', 'author': 'Peachey LD', 'year': '1996', 'journal-title': 'Scan Microsc'} / Scan Microsc / Correlated confocal and intermediate voltage electron microscopy imaging of the same cells using sequential fluorescence labeling, fixation, and critical point dehydration by Peachey LD (1996)
10.1016/0304-3991(95)00078-X
10.1016/S0091-679X(02)68005-7
10.1078/0065-1281-00595
10.1177/002215540104900701
10.1083/jcb.145.5.1009
10.1177/002215540305100602
{'key': 'e_1_2_1_51_1', 'first-page': '351', 'article-title': 'Correlative microscopy of ultrathin cryosections in placental research', 'volume': '121', 'author': 'Takizawa T', 'year': '2006', 'journal-title': 'Methods Mol Med'} / Methods Mol Med / Correlative microscopy of ultrathin cryosections in placental research by Takizawa T (2006)
10.1038/310285a0
10.1038/nbt0797-647
{'key': 'e_1_2_1_54_1', 'first-page': 'S16', 'article-title': "Imagining imaging's future", 'author': 'Tsien RY', 'year': '2003', 'journal-title': 'Nat Rev Mol Cell Biol Sep'} / Nat Rev Mol Cell Biol Sep / Imagining imaging's future by Tsien RY (2003)
10.1016/S0022-5320(70)90150-4
10.1016/0022-5320(72)90089-5
10.1002/hep.1840050427

Dates

Type	When
Created	18 years, 6 months ago (Feb. 5, 2007, 12:26 p.m.)
Deposited	1 year, 9 months ago (Nov. 15, 2023, 11:03 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 2, 2025, 1:23 a.m.)
Issued	18 years, 6 months ago (Feb. 5, 2007)
Published	18 years, 6 months ago (Feb. 5, 2007)
Published Online	18 years, 6 months ago (Feb. 5, 2007)
Published Print	18 years, 5 months ago (March 1, 2007)

Funders 0

None

BibTeX

@article{Braet_2007, title={Contribution of high‐resolution correlative imaging techniques in the study of the liver sieve in three‐dimensions}, volume={70}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.20408}, DOI={10.1002/jemt.20408}, number={3}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Braet, Filip and Wisse, Eddie and Bomans, Paul and Frederik, Peter and Geerts, Willie and Koster, Abraham and Soon, Lilian and Ringer, Simon}, year={2007}, month=feb, pages={230–242} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T05:23:16Z",
    "timestamp": 1754112196799
  },
  "reference-count": 56,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2007,
            2,
            5
          ]
        ],
        "date-time": "2007-02-05T00:00:00Z",
        "timestamp": 1170633600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2007,
        3
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Correlative microscopy has become increasingly important for the analysis of the structure, function, and dynamics of cells. This is largely due to the result of recent advances in light\u2010, probe\u2010, laser\u2010 and various electron microscopy techniques that facilitate three\u2010dimensional studies. Furthermore, the improved understanding in the past decade of imaging cell compartments in the third dimension has resulted largely from the availability of powerful computers, fast high\u2010resolution CCD cameras, specifically developed imaging analysis software, and various probes designed for labeling living and or fixed cells. In this paper, we review different correlative high\u2010resolution imaging methodologies and how these microscopy techniques facilitated the accumulation of new insights in the morpho\u2010functional and structural organization of the hepatic sieve. Various aspects of hepatic endothelial fenestrae regarding their structure, origin, dynamics, and formation will be explored throughout this paper by comparing the results of confocal laser scanning\u2010, correlative fluorescence and scanning electron\u2010, atomic force\u2010, and whole\u2010mount electron microscopy. Furthermore, the recent advances of vitrifying cells with the vitrobot in combination with the glove box for the preparation of cells for cryo\u2010electron microscopic investigation will be discussed. Finally, the first transmission electron tomography data of the liver sieve in three\u2010dimensions are presented. The obtained data unambiguously show the involvement of special domains in the de novo formation and disappearance of hepatic fenestrae, and focuses future research into the (supra)molecular structure of the fenestrae\u2010forming center, defenestration center and fenestrae\u2010, and sieve plate cytoskeleton ring by using advanced cryo\u2010electron tomography. Microsc. Res. Tech., 2007. \u00a9 2007 Wiley\u2010Liss, Inc.</jats:p>",
  "DOI": "10.1002/jemt.20408",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        2,
        5
      ]
    ],
    "date-time": "2007-02-05T17:26:58Z",
    "timestamp": 1170696418000
  },
  "page": "230-242",
  "source": "Crossref",
  "is-referenced-by-count": 102,
  "title": "Contribution of high\u2010resolution correlative imaging techniques in the study of the liver sieve in three\u2010dimensions",
  "prefix": "10.1002",
  "volume": "70",
  "author": [
    {
      "given": "Filip",
      "family": "Braet",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Eddie",
      "family": "Wisse",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Paul",
      "family": "Bomans",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Peter",
      "family": "Frederik",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Willie",
      "family": "Geerts",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Abraham",
      "family": "Koster",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lilian",
      "family": "Soon",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Simon",
      "family": "Ringer",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2007,
        2,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tice.2003.11.001"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2004.03.010"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.E02-05-0308"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060100111"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "117",
      "article-title": "Problems associated with preparations of whole mounts of cytoskeletons for high resolution electron microscopy",
      "volume": "3",
      "author": "Bell PB",
      "year": "1989",
      "journal-title": "Scan Electron Microsc"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1478-3231.2004.0974.x"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1523-1755.2005.00578_6.x"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1476-5926-1-1"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "201",
      "volume-title": "Atomic force microscopy: Methods and protocols in biomedical applications",
      "author": "Braet F",
      "year": "2004"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "944",
      "article-title": "Assessment of a method of isolation, purification and cultivation of rat liver sinusoidal endothelial cells",
      "volume": "70",
      "author": "Braet F",
      "year": "1994",
      "journal-title": "Lab Invest"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hep.1840210130"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.1996.72348.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.1997.1940755.x"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1098-1098(1997)8:2<162::AID-IMA3>3.0.CO;2-8"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "S575",
      "DOI": "10.1007/s003390051204",
      "article-title": "Comparison of fixed and living liver endothelial cells by atomic force microscopy",
      "volume": "66",
      "author": "Braet F",
      "year": "1998",
      "journal-title": "Appl Phys A"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.23.13635"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/jmicro/50.4.283"
    },
    {
      "key": "e_1_2_1_19_1",
      "first-page": "437",
      "volume-title": "The liver: Biology and pathobiology",
      "author": "Braet F",
      "year": "2001"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1471-2121-3-7"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.2003.01229.x"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1053/jhep.2003.50347"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.20065"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1047-8477(02)00019-9"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hep.1840210337"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(05)91024-0"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1991.tb03088.x"
    },
    {
      "key": "e_1_2_1_28_1",
      "first-page": "S43",
      "article-title": "The ultrastructure of cryo\u2010sections and intact vitrified cells the effects of cryoprotectants and acceleration voltage on beam induced bubbling",
      "volume": "5",
      "author": "Frederik PM",
      "year": "1991",
      "journal-title": "Scan Microsc"
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "476",
      "volume-title": "Cells of the hepatic sinusoid 6",
      "author": "Frederik PM",
      "year": "1997"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "B383",
      "volume-title": "Proceedings of the 12th European congress on electron microscopy",
      "author": "Frederik P",
      "year": "2000"
    },
    {
      "key": "e_1_2_1_31_1",
      "first-page": "715",
      "volume-title": "Protein\u2010protein interactions: A molecular cloning manual",
      "author": "Geerts WJC",
      "year": "2006"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth806"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0301-4622(02)00307-1"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.0022-2720.2005.01428.x"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02990514"
    },
    {
      "key": "e_1_2_1_36_1",
      "first-page": "S6",
      "article-title": "Electron microscopy in cell biology: Integrating structure and function",
      "author": "Koster AJ",
      "year": "2003",
      "journal-title": "Nat Rev Mol Cell Biol Sep"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.conb.2004.08.004"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.gt.3302326"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/a10000108"
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1070220203"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1997.3919"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(00)00255-5"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(00)01787-6"
    },
    {
      "key": "e_1_2_1_44_1",
      "first-page": "237",
      "article-title": "Correlated confocal and intermediate voltage electron microscopy imaging of the same cells using sequential fluorescence labeling, fixation, and critical point dehydration",
      "volume": "10",
      "author": "Peachey LD",
      "year": "1996",
      "journal-title": "Scan Microsc"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3991(95)00078-X"
    },
    {
      "key": "e_1_2_1_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(02)68005-7"
    },
    {
      "key": "e_1_2_1_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1078/0065-1281-00595"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/002215540104900701"
    },
    {
      "key": "e_1_2_1_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.145.5.1009"
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/002215540305100602"
    },
    {
      "key": "e_1_2_1_51_1",
      "first-page": "351",
      "article-title": "Correlative microscopy of ultrathin cryosections in placental research",
      "volume": "121",
      "author": "Takizawa T",
      "year": "2006",
      "journal-title": "Methods Mol Med"
    },
    {
      "key": "e_1_2_1_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/310285a0"
    },
    {
      "key": "e_1_2_1_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt0797-647"
    },
    {
      "key": "e_1_2_1_54_1",
      "first-page": "S16",
      "article-title": "Imagining imaging's future",
      "author": "Tsien RY",
      "year": "2003",
      "journal-title": "Nat Rev Mol Cell Biol Sep"
    },
    {
      "key": "e_1_2_1_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(70)90150-4"
    },
    {
      "key": "e_1_2_1_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-5320(72)90089-5"
    },
    {
      "key": "e_1_2_1_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hep.1840050427"
    }
  ],
  "container-title": "Microscopy Research and Technique",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.20408",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/full/10.1002/jemt.20408",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        15
      ]
    ],
    "date-time": "2023-11-15T16:03:47Z",
    "timestamp": 1700064227000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.20408"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2007,
        2,
        5
      ]
    ]
  },
  "references-count": 56,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2007,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jemt.20408"
  ],
  "URL": "http://dx.doi.org/10.1002/jemt.20408",
  "relation": {},
  "ISSN": [
    "1059-910X",
    "1097-0029"
  ],
  "subject": [],
  "container-title-short": "Microscopy Res &amp; Technique",
  "published": {
    "date-parts": [
      [
        2007,
        2,
        5
      ]
    ]
  }
}