DOI: 10.1002/jemt.1079. Elemental maps from EFTEM images using two different background subtraction models

Elemental maps from EFTEM images using two different background subtraction models

10.1002/jemt.1079

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

AbstractAcquisition of a great number of energy‐filtered images in a TEM (EFTEM) around the characteristic signal with a low energy‐selecting slit allows display of the electron energy loss (EEL)‐spectrum of regions of interest (ROIs) of a sample. These EEL‐spectra can be submitted to the different treatments already in use for electron energy loss spectroscopy (EELS). In particular, it is possible to fit the experimental background with different mathematical models, using images acquired below and above a characteristic ionization edge. After this fitting, elemental maps can be computed by subtraction of the extrapolated/interpolated background from the characteristic images. In this work, we compared two mathematical models for background fitting—the Egerton power law and the log‐polynomial law. We studied the low‐energy region (40–150 eV) and a higher‐energy region (350–600 eV) with the aid of software for interactive processing of EFTEM image series that we developed. The analyzed elements were the constitutive elements: iron, phosphorus, nitrogen, and oxygen in several biological materials. Two analytical TEMs, one equipped with a post‐column and the other with an in‐column spectrometer, were used. Our experimental results confirm that the power law is very sensitive to the value of the energy loss of the pre‐edge images when the background is computed by extrapolation. The log‐polynomial model is less sensitive than the power law model to the value of the energy loss of the pre‐edge images in the low energy region. For the oxygen K edge at 535 eV, it gives the best fit when it is combined with the interpolation method. The use of programs that facilitate the handling of EFTEM image series, and the controlled calculation of the background under the characteristic images, represent a step forward in the generation of elemental maps. Microsc. Res. Tech. 53:147–156, 2001. © 2001 Wiley‐Liss, Inc.

Bibliography

Quintana, C., Lechaire, J. P., Bonnet, N., Risco, C., & Carrascosa, J. L. (2001). Elemental maps from EFTEM images using two different background subtraction models. Microscopy Research and Technique, 53(2), 147â156. Portico.

Authors 5

C. Quintana (first)
J.P. Lechaire (additional)
N. Bonnet (additional)
C. Risco (additional)
J.L. Carrascosa (additional)

References 22 Referenced 7

{'key': 'e_1_2_1_2_1', 'first-page': '175', 'article-title': 'Optimum imaging parameters for elemental mapping in an energy filtering transmission electron microscope', 'volume': '92', 'author': 'Berger A', 'year': '1993', 'journal-title': 'Optik'} / Optik / Optimum imaging parameters for elemental mapping in an energy filtering transmission electron microscope by Berger A (1993)
10.1002/(SICI)1097-0320(19980301)31:3<217::AID-CYTO9>3.0.CO;2-H
{'key': 'e_1_2_1_4_1', 'first-page': '351', 'article-title': 'Developments in processing image sequences for elemental mapping', 'volume': '2', 'author': 'Bonnet N', 'year': '1988', 'journal-title': 'Scan Microsc'} / Scan Microsc / Developments in processing image sequences for elemental mapping by Bonnet N (1988)
{'key': 'e_1_2_1_5_1', 'volume-title': 'Electron energy‐loss spectroscopy in the electron microscope', 'author': 'Egerton RF', 'year': '1986'} / Electron energy‐loss spectroscopy in the electron microscope by Egerton RF (1986)
10.1016/S0304-3991(78)80030-8
10.1111/j.1365-2818.1994.tb03463.x
10.1051/mmm:0199200306051700
10.1016/S0304-3991(96)00071-X
10.1016/S0968-4328(97)00037-1
10.1111/j.1749-6632.1986.tb34541.x
{'key': 'e_1_2_1_12_1', 'first-page': '343', 'article-title': 'Spectral processing for parallel recording of elemental maps', 'volume': '2', 'author': 'Ottensmeyer FP', 'year': '1988', 'journal-title': 'Scan Microsc'} / Scan Microsc / Spectral processing for parallel recording of elemental maps by Ottensmeyer FP (1988)
{'key': 'e_1_2_1_13_1', 'first-page': '1531', 'article-title': 'Ferritin is associated to the aberrant tau filaments present in progressive supranuclear palsy', 'volume': '152', 'author': 'Perez M', 'year': '1998', 'journal-title': 'Am J Pathol'} / Am J Pathol / Ferritin is associated to the aberrant tau filaments present in progressive supranuclear palsy by Perez M (1998)
10.1016/S0304-3991(99)00021-2
10.1016/S0040-6090(98)00361-7
{'key': 'e_1_2_1_16_1', 'first-page': '563', 'article-title': 'Multivariate statistical analysis applied to X‐ray spectra and X‐ray mapping of liver cell nuclei', 'volume': '8', 'author': 'Quintana C', 'year': '1994', 'journal-title': 'Scan Microsc'} / Scan Microsc / Multivariate statistical analysis applied to X‐ray spectra and X‐ray mapping of liver cell nuclei by Quintana C (1994)
{'key': 'e_1_2_1_17_1', 'first-page': '175', 'article-title': 'Evaluation of the analytical and imaging performances of a non‐dedicated cryo‐TEM equipped with a parallel electron energy loss spectrometer (PEELS) and image filter (IF)', 'volume': '15', 'author': 'Quintana C', 'year': '1997', 'journal-title': 'J Trace Microprobe Tech'} / J Trace Microprobe Tech / Evaluation of the analytical and imaging performances of a non‐dedicated cryo‐TEM equipped with a parallel electron energy loss spectrometer (PEELS) and image filter (IF) by Quintana C (1997)
10.1016/S0968-4328(98)00011-0
{'key': 'e_1_2_1_19_1', 'first-page': '711', 'article-title': 'Optimization of phosphorus maps from EFTEM images by using different background models and multivariate statistical analysis (MSA) in chromatin', 'author': 'Quintana C', 'year': '1998', 'journal-title': 'Electron Microsc'} / Electron Microsc / Optimization of phosphorus maps from EFTEM images by using different background models and multivariate statistical analysis (MSA) in chromatin by Quintana C (1998)
10.1128/JVI.72.5.4022-4031.1998 / J Virol / Two types of viral‐related particles are found during TGEV morphogenesis by Risco C (1998)
10.1039/a908116j
10.1016/S0014-5793(98)00570-5
10.1016/0304-3991(90)90070-3

Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 4:21 p.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 2:57 a.m.)
Indexed	1 year, 9 months ago (Nov. 17, 2023, 9:47 a.m.)
Issued	24 years, 4 months ago (April 6, 2001)
Published	24 years, 4 months ago (April 6, 2001)
Published Online	24 years, 4 months ago (April 6, 2001)
Published Print	24 years, 4 months ago (April 15, 2001)

Funders 0

None

BibTeX

@article{Quintana_2001, title={Elemental maps from EFTEM images using two different background subtraction models}, volume={53}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.1079}, DOI={10.1002/jemt.1079}, number={2}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Quintana, C. and Lechaire, J.P. and Bonnet, N. and Risco, C. and Carrascosa, J.L.}, year={2001}, month=apr, pages={147–156} }

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